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American Journal of Medical and Biological Research. 2016, 4(3), 53-65
DOI: 10.12691/ajmbr-4-3-3
Open AccessArticle

Histomorphometrical Characterization of Skin of Native Cattle (Bos indicus) in Bangladesh

Md. Ershad Hossain1, Mohi Uddin2, S. Kumar Shil2, M. H. B. kabir3, , M. Showkat Mahmud4 and Kh. Nurul Islam2

1Department of Quality Assurance, Incepta Vaccine Limited, Dhaka, Bangladesh

2Department of Anatomy and Histology, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh

3Department of Microbiology and Parasitology, Sher-e-Bangla Agricultural University, Dhaka

4Animal Health research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh

Pub. Date: September 01, 2016

Cite this paper:
Md. Ershad Hossain, Mohi Uddin, S. Kumar Shil, M. H. B. kabir, M. Showkat Mahmud and Kh. Nurul Islam. Histomorphometrical Characterization of Skin of Native Cattle (Bos indicus) in Bangladesh. American Journal of Medical and Biological Research. 2016; 4(3):53-65. doi: 10.12691/ajmbr-4-3-3


Fresh skin samples of the native cattle (Bos indicus) of 6 male and 6 female were collected from ten regions of the body immediately after slaughtering aimed to know the regional and sex variation of skin components. The skin samples were fixed, processed and stained according to the standard histological procedure. The samples of vertical sections were stained by both Hematoxylin & Eosin stain and Van Geison & Verhoff’s stain. Transparent sheet and ocular micrometer was used for histomorphometric study. Histomorphometrical study of the skin revealed that the mean thicknesses of epidermis, papillary layer, reticular layer and total skin were 45.37 ± 0.59 µm, 0.55 ± 0.01 mm, 3.03 ± 0.09 mm and 3.57 ± 0.09 mm respectively. These thicknesses were significantly (P<0.05) varied among the body regions. The highest thicknesses for epidermis and papillary layer were found in back and ventral abdomen respectively and the lowest thicknesses in thigh and neck & shoulder respectively whereas the highest thicknesses for reticular layer and total skin were found in head and the lowest thicknesses in shoulder. The mean thicknesses of the epidermis, reticular layer and total skin were significantly (P<0.05)) higher in male than the female. The mean papillary layer thickness was insignificantly (P<0.05) higher in female than the male. The mean density of collagen fibre bundle, elastic fibre bundle, sebaceous gland, sweat gland and hair follicle per mm² were 302.93 ± 9.07, 4.96 ± 0.17, 4.22 ± 0.13, 4.57 ± 0.14 and 37.25 ± 1.77 respectively. The density of collagen and elastic fibre bundle were significantly (P<0.05) varied among the body regions. The highest density of collagen bundle was found in ventral abdomen and lowest density in thigh and the highest elastic bundle density in thigh and lowest in back & ventral abdomen. The density of collagen fibre, hair follicle and sebaceous gland were significantly higher (P<0.05) in male than the female. The density of sebaceous gland was significantly (P<0.05) highest in head and lowest in lateral abdomen. The density of sweat gland was insignificantly (P>0.05) highest in neck & shoulder and lowest in loin. The density of hair follicle was significantly (P<0.05) highest in back and lowest in ventral abdomen. In conclusion, male skin may be better than the female skin for the quality leather production. Collagen bundle is more compact in ventral abdomen that regions may be better for quality leather production, but the percentage of collagen did not revealed in this study.

Bangladeshi native cattle skin histology histomorphometry

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[1]  Mobini B, Faradonbeh KS (2012) A morphometric study on differentregions of the skin in lori-Bakhtiari sheep at different ages. International journal of plant, animal and environmental science. 2(4): 180-185.
[2]  Salehi M, Lavvaf A, Farahvash T (2013a) Skin Quality and Physical Properties of Leather Based on Sex, Age and Body Parts of Goats Reared on Sub‐Humid Hill Country. Iranian Journal of Applied Animal Science. 3(4): 853-857.
[3]  Adel R, Elboushi Y (1994) Poultry Feed from Waste. Hide and Tanning by Products. 154-156.
[4]  Widelitz RB, Tin-Xin J, Noveena A, Sheree A, Tin-Berreth EY, Hang-Sung J, Cheng-Ming C (1997) Molecular Histology in Skin Appendage Morphogenesis. Microscopy Research and Technique. 38: 452-465.
[5]  Genkovski D, Gerchev G (2007) Study of the skin histological structure in ewes from staroplaninska and thorough bred Tsigai. Biotechnology in Animal Husbandry. 23(5-6): 191–197.
[6]  Dellmann HD, Brown EM (1987) Text book of Veterinary Histology; 3rd editionn. Lea and Febiger. Philadelphia. 382-415.
[7]  Mannan AM (1973) Micro-anatomical and histochemical investigation of the normal and pathological skin of the hump of cattle. M. Sc (Vet. Science) thesis. Bangladesh Agricultural University, Mymensingh.
[8]  Copenhaver WM, Kelly DE, Wood RL (1978) Bailye’s Textbook of Histology. 17th Edition. Asian Edition. 423-445.
[9]  Ozfiliz N, Balikcier M, Erdost H, Zik B (2002) Histological and morphometric features of the skin of native and hybrid (f2) sheep. Turkish Journal of Veterinary and Animal Sciences. 26: 429-438.
[10]  Muralidharan MR, Ramesh V (2005). Histological and biochemical studies of the skin of cattle and buffalo. Indian Journal of Animal Research. 39: 41-44.
[11]  Razvi R, Suri S, Sarma K, Sharma R (2014) Histomorphological and histochemical studies on the different layers of skin of Bakerwali goat. Journal of Applied Animal Research. 1-6.
[12]  Sharma DN, Bharadwaj RL (1993) Regional variations in the thickness of the skin of adult yak. The Indian Veterinary Journal. 70: 437-438.
[13]  GoJdsbery S, Calhoun ML (1959). The comparative histology of the skin of Hereford and Aberdeen Angus cattle. American Journal of veterinary Research. 20: 61-68.
[14]  Kurtdede N, Asti RN (1999) The investigation on the skin structure of German Black Head, HampshireDown, Lincoln Longwool, White Karaman, Awassi and Konya Merino. Veterinary Journal of Ankara University. 46: 219-230.
[15]  Hafez ESE, Badreldin AL, Shafei MM (1955b) Skin structure of Egyptian buffaloes and cattle with particular reference to sweat glands. The Journal of Agricultural Science. 46(1): 19-30.
[16]  Hole MB, Bhosle NS Kapadnis PJ (2008) Histological Study of Skin Epidermis in Red Kandhari Cows. Indian Journal Animal Research. 42(1): 69-70.
[17]  Sultan GA (2012) Comparative histological and topographical study for the skin of head of male and female native black goat. AL-Qadisiya Journal of Veterinary Medicine Science. 11: 21-33.
[18]  Kobayashi A, Takehana K, Eerdunchaolu, Iwasa K, Abe M, Yamaguchi M (1999) Morphometric analysis of collagen; a comparative study in cow and pig skins. Anatomia, histologia, embryologia. 28: 235-238.
[19]  Mobini B (2013a) A quantitative evaluation of different regions of skin in adult Iranian native sheep. Veterinarni Medicina. 58(5): 260-263.
[20]  Bilgen G, Oktay G, Tokgoz Z, Guner G, Yalcin S (1999) Collagen content and electrophoretic analysis of type I collagen in breast skin of heterozygous naked neck and normally feathered commercial broilers. Turkish Journal of Veterinary and Animal Sciences. 23(5): 483-488.
[21]  Mitra SK (1963) Indian Hides and Skins-Histological Characteristics. Part - I.
[22]  Warren GH, James PJ, Neville AM (1983) A morphometric analysis of the changes with age in the skinsurface wax and the sebaceous gland area of Merino sheep. Australian Veterinary Journal. 60: 238-240.
[23]  Mobini B (2013g) Studies on the density of various dermal structures in adult rams and ewes. Bulgarian Journal of Veterinary Medicine. 16(1): 1-6.
[24]  PanYS (1963) Quantitative and morphological variation of sweat glands, skin thickness, and skin shrinkage over various body regions of Sahiwal Zebu and Jersey cattle. Australian Journal of Agricultural Research. 14(3): 424-437.
[25]  Hafez ESE, Badreldin AL, Shafei MM (1955a) The Hair Coat in Bovinae. Empire Journal of Experimental Agriculture. 23: 34-39.
[26]  Shafie MM (1954) Skin histology of Egyptian buffaloes and cattle. M. Sc. Thesis, Faculty of Agriculture. Cairo University. U.A.R.
[27]  Renani, HRA, Salehi M, Ebadi Z, Moradi S, Baghershah HR, Renani MYA, Ameli SH (2011b) Determination of hair follicle characteristics, density and activity of Iranian cashmere goat breeds. Small Ruminant Research. 95: 128-132.
[28]  Renani HRA, Salehi M, Ebadi Z, Moradi S (2010) Identification of hair follicle characteristics and activity of one and two humped camels. Small Ruminant Research. 90: 64-70.
[29]  Abbasi M, Gharzi A, Karimi H, Khosravinia H (2008) Effects of sex on histological characteristics of various areas of skin in an Iranian native breed of sheep. Journal of Animal and Veterinary Advances. 7: 1503-1505.
[30]  Andrews RN, Beattie AE, Dodds KG, Wuliji T, Montgomery GW (1998) Wool follicle traits of 1/2 Merino ½ Romney F1, and backcross 3/4 Merino 1/4 Romney gene mapping flocks. In: Proceedings of the New Zealand Society of Animal Production, New Zealand. 262-265.
[31]  Butler LG, Orazio RD, Ahlen K (1993) Some objective skin and fleece traits relating to pelt quality of Swedishpelt sheep. Small Ruminant Research. 12: 69-78.
[32]  Turner HG (1962) Effect of clipping the coat on performance of calves in the field.Australian Journal of Agricultural Research. 13: 180-192.
[33]  Renani HRA, Moradi S, Baghershah HR, Ebadi Z, Salehi M, Momen SMS, Renani MYA (2011a) Determination of wool follicle characteristics of Iranian sheep breeds. Asian-Australian Journal of Animal Sciences. 24: 1173-1177.
[34]  Kocamis H, Aslan S (2004) Histological and histometrical study on the structure of Tuj breed sheep skin. Kafkas Universitesi Veteriner Fakultesi Dergisi. 10(1): 91-98.