American Journal of Medical and Biological Research
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American Journal of Medical and Biological Research. 2013, 1(4), 95-98
DOI: 10.12691/ajmbr-1-4-2
Open AccessArticle

Xanthine Dehydrogenase Inhibition Stimulates Growth and Development of Human Brain Derived Cells

K. E. Danielyan1, and S. G. Chailyan1

1H. Buniatian Institute of Biochemistry, NAS RA, Paruyr Sevak Str, Yerevan, Armenia

Pub. Date: September 01, 2013

Cite this paper:
K. E. Danielyan and S. G. Chailyan. Xanthine Dehydrogenase Inhibition Stimulates Growth and Development of Human Brain Derived Cells. American Journal of Medical and Biological Research. 2013; 1(4):95-98. doi: 10.12691/ajmbr-1-4-2

Abstract

Background: Reactive Oxygen Species (ROS) play a critical role in development of number pathologies. Xanthine Oxidase (XO) as well as the Xanthine Dehydrogenase (XDH) are two enzymes responsible for the last steps of purine catabolism, hydroxylation of a wide variety pyrimidines, and the initiation of ROS synthesis. In our current experiments we have analyzed whether exogenously added allopurinol, not only the inhibitor of XO but also XDH, is capable for in vitro human brain derived cells growth, development and proliferation. Methods: We have used described by Mark Mattson’s neuronal cell culturing technique to seed and keep cells in vitro over 12 days. The death of the cells was visualized by the staining with Trypan Blue. Pixcavator and Image Tool programs served for the calculation of the cells’ number and size. Results: In comparison with the control group exogenously added xanthine as well as NAD+ or addition of NAD+ along with xanthine didn’t play any critical role. Only the group, treated with NAD+, xanthine and allopurinol promoted elevation of the cells’ number in the statistically significant way on the day 12th. Number of the death cells in comparison with the control groups in the cells groups treated with the xanthine, NAD+, as well as allopurinol was less, whereas in the NAD+ and xanthine treated group this number was higher than in the control group. Conclusion: We have concluded, that treatment with the low concentration of allopurinol, will guarantee the survival of the cells, decrease the number of the death cells and promote the proliferative processes. These conclusions are similar for allopurinol treatment in condition of exogenously stimulated activity of XO/XDH as well as endogenously stimulated activity of these enzymes.

Keywords:
Xanthine Oxidase Xanthine Dehydrogenase human brain derived cells cell culture allopurinol

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References:

[1]  Al-Gonaiah, M., Smith, R.A. and Stone, T.W., Xanthine oxidase-induced neuronal death via the oxidation of NADH: prevention by micromolar EDTA, Brain Res, 1280 (2009) 33-42.
 
[2]  Ben-Schachar, D., Mitochondrial dysfunction in schizophrenia: a possible linkage to dopamine, J Neurochem, 83 (2002) 1241-1251.
 
[3]  Berk, M., Copolov, D.L. and Dean, O., N-acetylcysteine for depressive symptoms in bipolar disorder-a double blind randomized placebo-controlled trial, Biol Psychiatry, 64 (2008) 468-475.
 
[4]  Danielyan, K.E. and Kevorkian, G.A., Xanthine oxidase activity regulates human embryonic brain cells growth, Biopolym. Cell, 27(5) 350-353.
 
[5]  Fatokun, A.A., Stone, T.W. and Smith, R.A., Hydrogen peroxide mediates damage by xanthine and xanthine oxidase in cerebellar granule neuronal cultures, Neurosci Lett, 416 (2007) 34-38.
 
[6]  Harris, C.M. and Massey, V., The oxidative half-reaction of xanthine dehydrogenase with NAD; reaction kinetics and steady-state mechanism, J Biol Chem, 272(45) (1997) 28335-41.
 
[7]  Ichikawa, M., Nishino, T., Nishino, T. and Ichikawa, A., Subcellular localization of xanthine oxidase in rat hepatocytes: high-resolution immunoelectron microscopic study combined with biochemical analysis, J Histochem Cytochem, 40(8) (1992) 1097-103.
 
[8]  Mattson, M.P. and Ruchlik, B., Cell culture of cryopreserved human fetal cerebral cortical and hippocampal neurons: neuronal development and resposes to trophic factors, Brain Research, 552 (1990) 2004-2212.
 
[9]  Nierenberg, A.A., Ostacher, M.J., Calabrese, J.R., Ketter, T.A., Marangell, L.B., Miklowitz, D.J., Miyahara, S., Bauer, M.S., Thase, M.E., Wisniewski, S.R. and Sachs, G.S., Treatment-resistant bipolar depression: a STEPBD equipoise randomized effectiveness trial of antidepressant augmentation with lamotrigine, inositol, or risperidone, Am J Psychiatry, 163 (2006) 210-216.
 
[10]  Rahman, A., Katzive, L., Stanley, K. and Henshaw, S.K., A Global Review of Laws on Induced Abortion, International Family Planning Perspectives1985-1997, 24 (1998).
 
[11]  Tennant, J., Evaluation of the Trypan Blue technique for determination of cell viability, Transplantation, 2 (1964) 685-694.