American Journal of Medical and Biological Research
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American Journal of Medical and Biological Research. 2016, 4(2), 20-25
DOI: 10.12691/ajmbr-4-2-2
Open AccessArticle

Plasma Cytokine Profiles as Predictive Biomarkers of HIV and Aids Progression among HIV Patients Attending Nakuru Provincial General Hospital, Kenya

Jane Nyambura Mugwe1, , Michael M. Gicheru2 and Joseph Mwatha3

1Department of Biological and Biomedical Sciences, Laikipia, University, Kenya

2Department of Zoological Sciences, Kenyatta University, Kenya

3Department of Biotechnology, Kenya Medical Research Institute, Kenya

Pub. Date: May 26, 2016

Cite this paper:
Jane Nyambura Mugwe, Michael M. Gicheru and Joseph Mwatha. Plasma Cytokine Profiles as Predictive Biomarkers of HIV and Aids Progression among HIV Patients Attending Nakuru Provincial General Hospital, Kenya. American Journal of Medical and Biological Research. 2016; 4(2):20-25. doi: 10.12691/ajmbr-4-2-2

Abstract

Introduction: Cytokines are produced by many cell types, mostly cells of the immune system, and act on diverse targets, often the white blood cells. They play a central role in the pathogenesis of many diseases including Acquired Immunodeficiency Disease Syndrome (AIDS). They reflect the local or systemic inflammatory setting, and could serve as predictive biomarkers in Human Immunodeficiency Virus (HIV) disease progression. Objective: The aim of this study was to identify cytokines measureable in blood plasma in recently diagnosed HIV individuals before the commencement of antiretroviral therapy. Study Population: Eighty individuals, both males and females, were recruited for this study that comprised of forty newly diagnosed with HIV-1; twenty HIV negative individuals; and twenty HIV positive individuals currently on highly active antiretroviral therapy (HAART). Method: Cytokines were measured using multiplex cytokine immunoassay. Five types of cytokines were detected. Data analyses were performed using Graph Pad Prism 6. Independent sample T tests were used to compare the cytokine means while Spearman Rank tests were used to test for correlations. Statistical analysis were done using SPSS version 17. Results: The study showed significantly (p=<0.001) higher levels of IL-12p70, TNF, IL-10, IL-6 and IL-1β among the newly diagnosed HIV patients compared to those on highly antiretroviral therapy and HIV negative patients. Conclusion: Identification of plasma cytokines could be useful predictive biomarkers of HIV disease progression.

Keywords:
plasma cytokines human immunodeficiency virus acute HIV infection predictive biomarkers HIV disease progression

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References:

[1]  Brennan, F.M. and Feldmann, M, (1992). Cytokines in autoimmunity. Current Opinion Immunology, 4:754-9.
 
[2]  Dayer, J.M.and Fenner, H, (1992). The role of cytokines and cytokine inhibitors in arthritis. In: Emery, P.ed. Bailliere’s clinical rheumatology, Bailliere Tindall, London. , 6:485-91.
 
[3]  Dinarello, C.A. (2000). "Proinflammatory cytokines", Chest, 118 (2): 5038.
 
[4]  Duprez, D.A., Neuhaus, J., Kuller, L.H., Tracy, R., Belloso, W.and De Wit, S, (2012). Inflammation,coagulation and cardiovascular disease in HIV-infected individuals. PLoS One, 7:e44454.
 
[5]  Folks, T.M., Justement, J. and Kinter, A, (1987). Cytokine-induced expression of HIV-1 in a chronically infected promonocyte cell line, Science, 238:800-2.
 
[6]  Fuster, D., Cheng, D.M., Quinn, E.K., Armah, K.A., Saitz, R.and Freiberg, M.S, (2014). Inflammatory cytokines and mortality in a cohort of HIV-infected adults with alcohol problems, AIDS, 28: 1059-1064.
 
[7]  Granowitz, E.V., Santos, A.A. and Poutsiaka, D.D, (1991). Production of interleukin-1 receptor antagonist during experimental endotoxaemia. Lancet, 338:1423-4.
 
[8]  Haissman Judith, Vestergaard, Lasse S., Sembuche Samuel, Erikstrup Christian, Lemnge Martha, Gerstoft Jan and Ullum Henrik, (2009). Plasma cytokine levels in Tanzanian HIV-infected Adults and the Effect, Journal of Acquired Immune Deficiency Syndrome, 52: (4) 493-497.
 
[9]  Joint United Nations Programme on HIV/AIDS (UNAIDS). GAP Report; 2013.
 
[10]  Joint United Nations Programme on HIV/AIDS (UNAIDS) (2014). Global Statistics, Fact sheet 2014 page 1-2.
 
[11]  Katsikis P.D., Mueller, Y.M. and Villinger, F. (2011). The Cytokine Network of Acute HIV Infection: A promising Target for Vaccines and Therapy to reduce viral set-point? PloS Pathogens 7(8): e1002055.
 
[12]  Kenya HIV Estimates (2014). Ministry of Health: National AIDS and STI Control Programme. 4-5.
 
[13]  Korn, T., Bettelli, E, Oukka, M. and Kuchroo,V.K. (2009).IL-17 and Th17 cells. Annual Review of Immunology, 27:485-517.
 
[14]  Kuller,L.H., Tracy, R., Belloso, W., De Wit, S., Drummond, F. and Lane, H.C. (2008).Inflammatory and coagulation biomarkers and mortality in patients with HIV infection. PLoS Med, 5:e203.
 
[15]  Lindi, Roberts; Jo-Ann S. Passmore, Carolyn Williamson, Francesca Little, Lisa M. Bebell, Koleka P. Mlisana, Wendy A. Burgers, Francois van Loggerenberg, Gerhard Walzl, Joel F. Djoba Siawaya, Quarraisha Abdool Karim; and Salim Abdool Karim. (2010). Plasma cytokine levels during acute HIV-1 infection predict HIV disease progression. AIDS, 24: 1251
 
[16]  Louie, S., Cai, J. and Law, R. (1995).Effects of interleukin-1 and interleukin-1 receptor antagonist in AIDS-Kaposi’s sarcoma. Journal of AIDS Human Retrovirus, 8:455-60.
 
[17]  Martyn A. French, Alessandro Cozzi-Lepri, Roberto C. Arduino, Margaret Johnson, Amit C. Achira and Alan Landay. (2015). Plasma levels of cytokines and chemokines and the risk of mortality in HIV-infected individuals: a casecontrol analysis nested in a large clinical trial. AIDS, 29:847-851.
 
[18]  Maury, C.P.J. and Labdevirta, J. (1990). Correlation of serum cytokine levels with hematological abnormalities in human immunodeficiency virus infection. Journal Internals of Medicine, 227:253-7.
 
[19]  Mosmann, T.R.and Fong, T.A. (1989). Specific assays for cytokine production by T cells. Journal of Immunological Methods, 116:151-158.
 
[20]  Ohlsson, K., Bjork, P. and Bergenfeldt, M. (1990). Interleukine-1 receptor antagonist reduces mortality from endotoxin shock. Nature, 348:550-2.
 
[21]  Okusawa, S., Gelfiand, J.A. and Ikejima, T. (1988). Interleukin-1 induces a shock-like state in rabbits. Synergism with tumor necrosis factor and the effect of cyclooxygenase inhibition. Journal of Clinical Investigations, 81:1162-7.
 
[22]  Stylianou, E., Aukrust, P, and Kvale, D. (1999). IL-10 in HIV infection: increasing serum IL-10 levels with disease progression-down-regulatory effect of potent anti-retroviral therapy. Clinical Immunology, 116:
 
[23]  Sudman, S. (1976). Applied Sampling. New York. Academic Press.
 
[24]  Tudela,E.V., Singh, M.K., Lagman, M., Ly, J., Venkeraraman, V. et al., (2014). Cytokines Levels in plasma samples of individuals with HIV infection. Austin Journal of Clinical Immunolygy, 1(1):1003.
 
[25]  Weiss, J.M. and Sundar, S.K. (1992). Interlleukin-1: effects in the central nervous system and relevance to AIDS. Clinical Neoropharmacol, 15:661A-2A
 
[26]  World Health Organization (WHO). Global HIV/AIDS response. 2013.
 
[27]  59th WMA General Assembly, Seoul. (2008).World Medical Association Declaration of Helsinki: ethical Principles for Medical Research involving Human subjects. (2008).
 
[28]  Yamane, T. (1967). Statistics: An Introductory Analysis.2nd ED., New York, Harper and Rao. 886.