American Journal of Medical and Biological Research
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American Journal of Medical and Biological Research. 2015, 3(4), 117-123
DOI: 10.12691/ajmbr-3-4-7
Open AccessArticle

Osteometric Assessment of the Mastoids for Gender Determination in Jordanians by Discriminant Function Analysis

Amin W1, , Saleh M-W2, Othman D3, Salhab D3 and Thunaibat H.3

1Department of Prosthodontics, Faculty of Dentistry, University of Jordan, West Amman Province 11942, Amman, Jordan.

2Postgraduate Section, Department of Prosthodontics, Faculty of Dentistry, University of Jordan, Wes Amman Province 11942, Amman, Jordan

3Intern Dental Practitioner, Jordan University Hospital, West Amman Province 11942, Amman, Jordan

Pub. Date: June 26, 2015

Cite this paper:
Amin W, Saleh M-W, Othman D, Salhab D and Thunaibat H.. Osteometric Assessment of the Mastoids for Gender Determination in Jordanians by Discriminant Function Analysis. American Journal of Medical and Biological Research. 2015; 3(4):117-123. doi: 10.12691/ajmbr-3-4-7


The purpose of this study was, firstly, to characterize the mastoid process in terms of its size, its surface area, its flare and its medial convergence angle on a sample of Jordanian subjects; and secondly, to assess the validity of the mastoid process in determining the gender of the study subjects. The sample of the study comprised 192 3D skull images with known sex, 96 each male and female, those were obtained by rendering DICOM images from cone-beam computerized tomography of the subjects. Radiographic measurements, using customized software, accurately characterized the subjects’ mastoids. Discriminant function analysis revealed that mastoid process correctly classified the sex in 90.6 percent of the subjects and the intermastoidale distance was found to be the best determinant for sex. A discriminant function equation specific for young and middle age Jordanians has been derived from the mastoid variables.

mastoid process discriminant function mastoid flare mastoid medial convergence surface rendering cone beam CT 3D skull image Solid Planner software

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[1]  Balci Y, Yavuz M F, Cagdir S. Predictive accuracy of sexing the mandible by ramus flexure. Homo, 2005; 55: 229-37.
[2]  Krogman W M, Iscan M Y. The human skeleton in forensic medicine. C.C. Thomas Publications: Springfield, IL. 1986.
[3]  Novotony V, Iscan M Y, Loth S R. Morphologic and osteometric assessment of age, sex and race from the skull. In Iscan M Y and Helmer (eds.): Forensic analysis of the skull. New York; Willy Liss, 1993; pp. 71-88.
[4]  Biggerstaff RH.. Craniofacial characteristics as determination of age sex & race in forensic dentistry. Dent Clin North Am. 1977; 21(1): 85-97.
[5]  Krogman WM. The human skeleton in forensic medicine. Springfield. Illinois, USA. Charles C. Thomas Pub Ltd, 1962; 114-22.
[6]  Scheuer L. Application of osteology to forensic medicine. Clin Anat 2002; 15(4): 297-312.
[7]  Iscan MY. Forensic anthropology of sex and body size. Forensic Science International 2005; 147: 107-12.
[8]  Mall G. Sex determination and estimation of stature from the long bones of the arm. Forensic Science International 2001; 117: 23-30.
[9]  Kalmey JK, Rathbun TA.. Sex determination by Discriminant function analysis of the petrous portion of temporal bone. J Forensic Sci. 1996; 41(5): 865-7.
[10]  Graw M, Czarnetzki A, Haffner HT. The form of the supraorbital margin as a criterion in identification of sex from the skull: investigations based on modern human skulls. Am J Phy Anthropol 1999; 108(1): 91-6.
[11]  Graw M. Significance of the classical morphological criteria for identifying gender using recent skulls. Forensic Sci Communications 2001; 3(1): 1-8.
[12]  Williams BA, Rogers T. Evaluating the accuracy and precision of cranial morphological traits for sex determination. J Forensic Sci. 2006; 51(4): 729-35.
[13]  Kranioti EF, Iscan MY, Michalodimitrakis M.. Craniometric analysis of the modern Cretan population. Forensic Science International 2008; 180: 110. e1-e5.
[14]  Pavia LAS and Segre M. Sexing the human skull through the mastoid process. Rev Hop Clin Fac Med Sao Paulo. 2003; 58(1): 15-20.
[15]  Patil KR, Mody RN. Determination of sex by discriminant function analysis and stature by regression analysis: a lateral cephalometric study. Forensic Sci Int.; 2005; 147(2-3): 175-80.
[16]  World Medical Association “WMA”. Declaration of Helsinki–Ethical Principles for Medical Research Involving Human Subjects. Proceedings of the 18th General Assembly, Helsinki, Finland, Report; 1-8, June 1964.
[17]  Amin W, Saleh M-W. Accuracy of a customized volumetric rendering program in linear measurement of cone beam and multi-slice computed tomography derived three-dimensional images. J Oral and Maxillofacial Radiology 2015; 3 (2):33-38.
[18]  Kemkes A, Gobel T. Metric assessment of “mastoid triangle” for sex determination: A validation study. J Forensic Sci. 2006; 51(5): 985-989.
[19]  Nagaoka T, Shizushima A, Sawada J, Tomo S, Hoshino K, Sato H, Hirata K. Sex determination using mastoid process measurements: standard for Japanese human skeletons of the medieval and early modern periods. Anthropological Science (Japanese series).2008; 116(2):105-113.
[20]  Bernard K, Moore-Jansen P. Quantifying male and female shape variation in the mastoid region of the temporal bone. Proceedings of the 5th Annual GRASP symposium, Wichita State University. 2009; 80-81.
[21]  Sumati, Patnaik VVG, Ajay P. Determination of sex from mastoid process by discriminant function analysis. J Anat Soc India. 2010; 59(2): 222-8.
[22]  Suranjittham S, Vichairat K, Prasitwattanaseree S, Mahakkanukrauh P.. Thai human skeleton sex identification by mastoid process measurement. Chiang Mai Med J 2011; 50(2): 43-50.
[23]  Gangrade P, Yadav R, Jain LK. Sexing the human skull using mastoid process measurements. Research and Review J Med H Sci. 2013; 2(1): 35-9.
[24]  Gupta A, Banerjee A, Kumar A, Rao S, Jose J. Discriminant function analysis of mastoid measurements in sex determination. J life Sci. 2012; 4(1): 1-5.
[25]  Keen JA. Sex differences in skulls.Am J Phy Anthropol. 1950;8: 65-79.
[26]  Giles E. and Elliot O. Sex determination by discriminant function analysis of crania. Am J Phy Anthropol 1963; 21: 53-8.
[27]  Kajanoja P. Sex determination of Finnish crania by discriminant function analysis. Am J Phy Anthropol 1966; 24: 29.
[28]  Rogers TL. Determining the sex of human remains through cranial morphology. J Forensic Sci. 2005; 50(3): 493-500.