American Journal of Medical and Biological Research
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American Journal of Medical and Biological Research. 2015, 3(4), 113-116
DOI: 10.12691/ajmbr-3-4-6
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Analysis of expression of mRNA Matrix Metalloproteinase (MMP)-8 gene and Tissue Inhibitors of Metalloproteinase (MMP)-1 in Intraperitoneal Adhesion after Usage of Hyaluronate acid-Carboxymethylcellulose (HA-CMC) or Virgin Coconut Oil (VCO)

Jimmy Panelewen1, Aryono Djuned Pusponegoro2, Ibrahim Labeda3, Andi Asadul Islam3 and Mochammad Hatta4,

1Department of Surgery, Faculty of Medicine, Sam Ratulagi University, Manado, Indonesia

2Department of Surgery, Faculty of Medicine, Indonesia University, Jakarta, Indonesia

3Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

4Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

Pub. Date: June 22, 2015

Cite this paper:
Jimmy Panelewen, Aryono Djuned Pusponegoro, Ibrahim Labeda, Andi Asadul Islam and Mochammad Hatta. Analysis of expression of mRNA Matrix Metalloproteinase (MMP)-8 gene and Tissue Inhibitors of Metalloproteinase (MMP)-1 in Intraperitoneal Adhesion after Usage of Hyaluronate acid-Carboxymethylcellulose (HA-CMC) or Virgin Coconut Oil (VCO). American Journal of Medical and Biological Research. 2015; 3(4):113-116. doi: 10.12691/ajmbr-3-4-6

Abstract

Background: Purpose : To understand the expression of matrix metalloproteinase (MMP)-8 mRNA gene and tissue inhibitors of metalloproteinase (TIMP)-1 in normal peritoneum tissue and intraperitoneal adhesion tissue after the usage of virgin coconut oil (VCO) and hyaluronate Acid-carboxymethylcellulose (HA-CMC). Methods: The research is using an experimental lab rat “rattus norvegicus strain wistar” which is divided into 3 groups. Laparotomy (pre) is done to gain parietal peritoneum tissue at the lower left quadrant of the abdomen, in order to obtain the expression of mRNA gene MMP-8 and TIMP-1. First group was control group, second group was treated with VCO, while the third group was treated with HA-CMC. After two weeks period, another relaparotomy (post) was held to obtain the adhesion tissue and to run another examination on the MMP-8 mRNA gene expression and TIMP-1. Results: There is a meaningful decrease for the MMP-8 mRNA gene expression in the peritoneum tissue for the control group at the pre and post laparotomy (p<0,001). There is a meaningful over expression of TIMP-1 mRNA gene expression at pre dan post laparatomy of control group (p<0,001). While on the other hand, at the VCO nor HA-CMC group, there is an insignificant increase of MMP-8 mRNA gene expression (p=0,075 and p=0,791) and insignificant decrease of TIMP-1 mRNA gene expression (p=0,015 and p=0,368). Conclusion: The decrease of MMP-8 mRNA gene expression and the increasing of TIMP-1 mRNA gene expression of control group shows a high risk of adhesion incidence. Although it is not statistically meaningful, the result of MMP-8 mRNA and TIMP-1 mRNA gene expression of VCO and HA-CMC indicates that there is a decrease of intraperitoneal adhesion risks, and the nature of VCO anti adhesive compared to HA-CMC.

Keywords:
intraperitoneal adhesion MMP-8 mRNA gene TIMP-1 mRNA gene VCO HA-CMC

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