American Journal of Medical and Biological Research
ISSN (Print): 2328-4080 ISSN (Online): 2328-4099 Website: http://www.sciepub.com/journal/ajmbr Editor-in-chief: Apply for this position
Open Access
Journal Browser
Go
American Journal of Medical and Biological Research. 2014, 2(1), 18-25
DOI: 10.12691/ajmbr-2-1-4
Open AccessArticle

Preliminary Results of Influence of Nonionizing Electromagnetic Radiation on Tumor and Healthy DNA and Role of Water

Vitali Kalantaryan1, , Radik Martirosyan1, Yuri Babayan2, Lusine Nersesyan3 and Hrachya Stepanyan3

1Microwave Radiophysics and Telecommunication, Yerevan State University, Yerevan, Armenia

2Medical Physics, Yerevan State Medical University, Yerevan, Armenia

3Fine Organic Chemistry Institute of National Academy Sciences of Armenia, Yerevan, Armenia

Pub. Date: February 25, 2014

Cite this paper:
Vitali Kalantaryan, Radik Martirosyan, Yuri Babayan, Lusine Nersesyan and Hrachya Stepanyan. Preliminary Results of Influence of Nonionizing Electromagnetic Radiation on Tumor and Healthy DNA and Role of Water. American Journal of Medical and Biological Research. 2014; 2(1):18-25. doi: 10.12691/ajmbr-2-1-4

Abstract

Unlike now widely used traditional methods of treatment of tumors by means of ionizing radiation and the chemotherapy, the method of the use of low intensity electromagnetic fields (EMF) is non-ionizing and non-invasive and hence is completely deprived of any harmful side effects. The present study was undertaken to investigate whether low-intensity EMFs can suppress tumoral cells growth in vivo without cytostatic agents. The course of influence of EMFs started 3 days before transplantation in order to raise activity of the animals’ immune system. On the fourth day animals were injected by sarcoma-37 and daily exposure was continued during 15 days. For study of the effect of irradiation on the secondary structure of DNA, in the experiments DNA isolated from the liver of healthy mice (hDNA) as well as from the tumor sarcoma 37 (tDNA) was used. After 15 sessions of exposure without cytostatic drugs, at animals of the irradiated 0,5 hour was observed an inhibition of tumor growth by 33.5% compared with a control group and a sharp suppression of the level of DNA-methylation in 2.1 times. The tDNA has the high level of methylation (4.7 mol%), which after 0.5 hour daily exposure becomes (2.2 mol%) close to the corresponding value for hDNA (1.9 mol%). Differential melting curves (DMC) of tDNA are shifted relatively DMC of the hDNA to lower temperatures, and in the DMC of tDNA the additional peaks in the 52-60ºC range are appeared, which are absent for DMC of liver hDNA. The obtained results are correlated with the spectrophotometric data. Under the influence of EMFs the values of temperature and interval of melting of tDNA are changed and approach to the corresponding values of hDNA. Presented preliminary results have demonstrated the potential clinical application of low power EMFs for clinical oncology in the treatment of malignancies. The changes of physical-chemical properties of tumoral and healthy DNA under amplitude modulated radiation at 64.5 GHz and possible mechanisms of these changes have been investigated and discussed.

Keywords:
weak electromagnetic fields antitumor effect in vivo demethylation DNA cytostatic drugs sarcoma

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Rojavin, M.A. and Ziskin, M.C., “Medical applications of millimeter waves,” QJ Med, 91, 57-66, 1998.
 
[2]  Pakhomov, A.G. and Murphy, M., “Low intensity millimeter waves as a novel therapeutic modality,” IEEE Trans Plasma Sci, 28, 34-40, 2000.
 
[3]  Pletnev, S.D., “The use of millimeter band electromagnetic waves in clinical oncology,” Crit Rev Biomed Eng, 28, 573-87, 2000.
 
[4]  Gapeyev, A.B. and Chemeris, N.K., “Model approach to the analyses of effects of modulated electromagnetic radiation on animal cells,” Biophysics, 45, 299-312, 2000.
 
[5]  Tadevosyan, H.H., Kalantaryan, V.P. and Trchounian, A.H., “Extremely high frequency electromagnetic radiation enforces bacterial effects of inhibitors and antiobiotics,” Cell Biochemestry & Biophysics, 51(2-3), 97-103, Jul.2008.
 
[6]  Kalantaryan, V.P., Vardevanyan, P.O., Babayan, Y.S., Gevorgyan, E.S., Hakobyan, S.N. and Antonyan, A.P., “Influence of low intensity coherent electromagnetic millimeter radiation (EMR) on aqua solution of DNA”, Progress In Electromagnetics Research Letters, 13, 1-9, 2010.
 
[7]  Babayan, Y.S., Tadevosyan, A.A. and Kalantaryan, V.P., “Influence of millimeter range coherent electromagnetic radiation on some properties of solutions of DNA”, Biomedical Radioelectronics, No2, 52-57, Febr.2009.
 
[8]  Shenberg, A.S., Uzbekov, M.G., Shihov, S.N., Bazyan, A.S., and Chernyakov, G.M., “Some neyrotrop effects of low intensity electromagnetic waves on the rats with different typological pecularities of the highest neural activity”, Journal of the Highest Neural Activity, 50, 867-77, 2000.
 
[9]  Minasyan, S.M., Grigoryan, G.Y., Saakyan, S.G., Akhumyan, A.A., and Kalantaryan, V.P., “Effects of the action of microwave-frequency electromagnetic radiation on the spike activity of neurons in the supraoptic nucleus of the Hypothalamus in rats”, Neuroscience and Behavioral Physiology, 37(2), 175-80, 2007.
 
[10]  Sitko SP, Mkrtchyan LN. Introduction to Quantum Medicine. Kiyev: Pattern;1994.
 
[11]  Logani, M.K., Szabo, I., Makar, V.R., Bhanushali, A., Alekseev, S.I., and Ziskin, M.C., „Effect of Millimeter wave irradiation on tumor metastasis”, Bioelectromagnetics, 27, 258-64, 2007.
 
[12]  Kalantaryan, V.P., Babayan, Y.S., and Tadevosyan, A.A., “Investigation of the binding of antitumor compouds of Mitoxantrone and Amentantrone with the DNA-irradiated millimeter electromagnetic waves”, in UICC World Cancer Congress, Geneva; p.82 POS- A288..2008a.
 
[13]  Kalantaryan, V.P., Babayan, Y.S., and Gharibyan, J.V., “Cross influence of Doxorubicin antitumour drug and millimeter electromagnetic waves on structure of tumour DNA”, in UICC World Cancer Congress, Geneva; PUB-306. 2008b.
 
[14]  Barbault, A., Costa, F., Bottger, B., Munden, R., Bomholt, F., Kuster, N., et al. “Amplitude-modulated electromagnetic fields for the treatment of cancer: discovery of tumor-specific frequencies and assessment of a novel therapeutic approach”, J Exp Clin Cancer Res, 28(1), 51-60, Apr.2009.
 
[15]  Costa, F.P., de Oliveira, A.C., Meirelles, R., Machado, M.C., Zanesco, T., Surjan, R., et al. “Treatment of advanced hepatocellular carcinoma with very low levels of amplitude-modulated electromagnetic fields”, Br J Cancer, 105(5), 640-48, Aug. 2011.
 
[16]  Zimmerman JW, Pennison MJ, Brezovich I, Yi N, Yang CT, Ramaker R, et al. “Cancer cell proliferation is inhibited by specific modulation frequencies”, Br J Cancer, 106(2), 307-13, Jan. 2012.
 
[17]  Kuzmenko, A.P., Solovyev, E. and Bundyuk, L.S., “Features of course of tumoral process at influence low power microwave radiations on acupuncture points in experiment”, Experimental oncology, 14(1), 72-6, 1992.
 
[18]  Chidichimo, G., Beneduci, A., Nicoleta, M., Critelli, M., De Rose, R., Tkatchenco, Y., et al. “Selective inhibition of tumor cells growth by low power millimeter waves”, Anticancer Research 22, 1681-8, 2002.
 
[19]  Burnham, C.M., “Components of Listeria and Escherichia coli have been used to prime the immune system in a novel approach to fighting cancer”, Drug Discover Today, 2(8), 54-8, 1992.
 
[20]  Partha, M.D. and Rakesh, S., “DNA methylation and cancer”, J.Clinical Oncology, 22(22), 4632-42, 2004.
 
[21]  Chernov, V.A., Methods of experimental chemotherapy. Moscow: Medicine press; 1971.
 
[22]  Makar, V.R., Logani, M.K., Bhanushali, A., Alekseev, S.I., and Ziskin, M.C., “Effect of Cyclophosphamide and 61.22GHz Millimeter Waves on T-Cell, B-Cell, and Macrophage Functions”, Bioelectromagnetics, 27, 458-66, 2006.
 
[23]  Gapeyev, A.B., Sokolov, P.A. and Chemeris, N.K., “A study of absorption of energy of the extremely high frequency electromagnetic radiation in the rat skin by various dosimetric methods and approaches”, Biophysics, 47, 759-768, 2002.
 
[24]  Gapeyev, A.B., Mikhailik, E.N. and Chemeris, N.K., “Anti-inflammatory effects of low-intensity extremely high-frequency electromagnetic radiation: Frequency and power dependence”, Bioelectromagnetics, 29, 197-206, 2008.
 
[25]  Vanyushin, B.F., Masin, A.H., Vasiliev, V.R. and Belozersky, A.N., “The content of 5-methylcytosine in animal DNA: the species ant tissue specificity”, Biochem. Et Biophys. Acta, 299, 397-403, 1973.
 
[26]  Babayan, YS, Khudaverdyan NV, Sngryan HE, Gharibyan JV. “Influence of Imidazen in a combination with RNA on structure of DNA sarcoma 45”, Biophysica, 42(1), 125-8, 1997.
 
[27]  Babayan, Y.S., Vardevanyan, P.O., and Gharibyan, J.V., “Application of a method of melting in revealing of distinctions between DNA from normal and tumoral tissues”, Biophysica, 29(2), 313-4, 1984.
 
[28]  Chih-Lin Hsieh, “The de novo methylation activity of Dnmt3a is distinctly different than that of Dnmt1”, BMC Biochemistry, 6, 6, 2005.
 
[29]  Li, E. and Bird, A., DNA methylation in mammals Epigenetics-Cold. Spring Harbor (NY): 2007.
 
[30]  Barret, J.M., Salle, B., Provot, C. and Hill, B.T., “Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescense microplate assay”, Carcinogenesis, 18, 2441-5, 2005.
 
[31]  Teppone, M.V. and Avagyan, R.S., “Extremely high frequency (EHF) therapy in oncology”, Millimeter waves in biology and medicine, 29(1), 3-19, 2003.
 
[32]  Bhattacharya, S., Ramchandani, S., Cervoni, N. and Szyf, M., “A mammalian protein with specific demethylase activity for mCpG DNA”, Nature, 397, 579-83, 1999.
 
[33]  V., Martirosyan, R., Nersesyan, L., Aharonyan, A., Danielyan, I., Stepanyan, H. at al, “Effect on tumoral cells of low intensity electromagnetic waves”, Progress in Electromagnetics Research Letters, 20, 97-105, 2011.
 
[34]  Babayan, Y.S. and Gharibyan, J.V., “Structural features of DNA of sarcoma 45 tumor”, Biophysica, 35(4), 592-6, 1990.
 
[35]  Belyaev, I.Ya., Alipov, Y.D., Shcheglov, V.S. and Lystsov, V.N., “Resonance effect of microwaves on the genome conformational state of E. coli cells”, Z Naturforsch С, 47(7-8), 621-7, 1992.
 
[36]  Khabarova, O., The influence of cosmic weather on the Earth.// International School of Space Science. Book of Proceedings of the 10th course on "Sun-Earth Connection and Space Weather" (L'Aquila 2000), Society Italiana di Fisica, 56-62, 2001.
 
[37]  Weinsburg, S., “DNA Helix found to oscillate in resonance with microwaves”, Science News, 125(16), 248-53, 1984.
 
[38]  Petrosyan, V.I., Sinitsin, N.I., Elkin. V.A., Devyatkov, N.D., Gulyayev, Y.V., Betskii, O.V., et al.”Role of resonance molecular-wave processes in the nature and their use for the control and corrections of a condition of ecological systems”, Biomeditsinskaya Radioelectronica, No.5-6, 62-105, 2001.
 
[39]  Adams, P.L. and Eason, R., “Increased GC content of DNA stabilizes methyl CpG dinucleotides”, Nucleic Asids Res, 12(14), 5869-77, 1984.
 
[40]  Behe, M. and Felsenfeld, G., “Effects of methylation on a synthetic polynucleotide: The B Z transition in poly(dG-m5C)”, Proc. Nat. Acad. Sci. USA, 78(3), 1619-23, 1981.
 
[41]  Rodionov, B.N., “Energo-informational effect of low-energetic electromagnetic radiations on biological objects”, New Medical Technologies Report, 6(3-4), 24-9, 199.