American Journal of Infectious Diseases and Microbiology
ISSN (Print): 2328-4056 ISSN (Online): 2328-4064 Website: http://www.sciepub.com/journal/ajidm Editor-in-chief: Maysaa El Sayed Zaki
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American Journal of Infectious Diseases and Microbiology. 2017, 5(4), 132-136
DOI: 10.12691/ajidm-5-4-2
Open AccessArticle

Staphylococcus pseudintermedius Isolated from Two Dog Cases with Ophthalmic Lesions

Maha I. Hamed1, , Terri L. McCalla2, Wendy M. Townsend3 and Mohamed N. Seleem4

1Animal Medicine Department, Faculty of Veterinary Medicine- Assiut University, Assiut, Egypt

2Animal Eye Care LLC, Bellingham WA, USA

3Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA

4Department of Comparative Pathobiology, Collage of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA

Pub. Date: November 02, 2017

Cite this paper:
Maha I. Hamed, Terri L. McCalla, Wendy M. Townsend and Mohamed N. Seleem. Staphylococcus pseudintermedius Isolated from Two Dog Cases with Ophthalmic Lesions. American Journal of Infectious Diseases and Microbiology. 2017; 5(4):132-136. doi: 10.12691/ajidm-5-4-2

Abstract

Two dog cases with history of recurrent bacterial conjunctivitis were submitted to Purdue University for bacterial culture and sensitivity testing. The bacterial cultures were also tested for their ability to form a biofilm. Biofilm forming Staphylococcus pseudintermedius was isolated from the two cases and identified by Vitek2 and PCR analysis. The bacterial antibiotic sensitivity testing revealed that Isolate one was susceptible to ticarcillin, ceftiofur, cefazolin, moxifloxacin, amikacin and rifampicin with MICs ranging 0.03125-4 μg/ml. Isolate two was susceptible to ceftiofur, gatifloxacin, cefazolin, moxifloxacin, tetracycline, doxycycline and rifampicin with MICs ranging <0.0625–4 μg/ml. Meanwhile, the biofilm antibiotic sensitivity testing revealed that Isolate one biofilm was resistant to all antibiotics except for ceftiofur and moxifloxacin with MICs <0.25 and 8 μg/ml respectively. Isolate two biofilm was resistant to all antibiotics except for tetracycline, amikacin, doxycycline, chloramphenicol and rifampicin with MICs ranged from <0.125 to 8 μg/ml. Case one was resolved with topical cefazolin therapy. Meanwhile case two showed complete recovery after treated with topical levofloxacin plus oral doxycycline.

Keywords:
Staph. pseudintermedius biofilm dogs ophthalmic lesions

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