American Journal of Infectious Diseases and Microbiology
ISSN (Print): 2328-4056 ISSN (Online): 2328-4064 Website: http://www.sciepub.com/journal/ajidm Editor-in-chief: Maysaa El Sayed Zaki
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American Journal of Infectious Diseases and Microbiology. 2016, 4(6), 118-122
DOI: 10.12691/ajidm-4-6-2
Open AccessArticle

Identification of Virulence of Bacillus anthracis from Soil by Multiplex PCR Technique in the South Sulawesi Province of Indonesia

Zainal Abidin Kholilullah1, , Muh. Nasrum Massi2, Lucia Muslimin1, Rizalinda Sjahril2, Firdaus Hamid2 and Isra Wahid3

1Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

2Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

3Department of Parasitology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

Pub. Date: December 28, 2016

Cite this paper:
Zainal Abidin Kholilullah, Muh. Nasrum Massi, Lucia Muslimin, Rizalinda Sjahril, Firdaus Hamid and Isra Wahid. Identification of Virulence of Bacillus anthracis from Soil by Multiplex PCR Technique in the South Sulawesi Province of Indonesia. American Journal of Infectious Diseases and Microbiology. 2016; 4(6):118-122. doi: 10.12691/ajidm-4-6-2

Abstract

Anthrax is a disease caused by bacteria that attack herbivores and acute. The disease is caused by Bacillus anthracis is a gram-positive bacteria can form endospores and capable dormants for decades in the soil. This study aims to detect the presence of germs that cause anthrax in the soil at several locations ranch scene the disease in six districts / cities in South Sulawesi Province, Republic of Indonesia. Then determine the virulence of Bacillus anthracis were found. The method in this research is by taking 32 samples of soil in the farm ever anthrax disease. The land taken from test using multiplex polymerase chain reaction (m-PCR), which uses three pairs of primer, namely (Bac R, Cap and Lef) to detect the presence of Bacillus anthracis same time know the status of the virulence of the bacteria. The results of this study found two positive samples containing Bacillus anthracis and is known that both these types of agents were found to be virulent.

Keywords:
multiplex polymerase chain reaction virulence Bacillus anthracis

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