American Journal of Infectious Diseases and Microbiology
ISSN (Print): 2328-4056 ISSN (Online): 2328-4064 Website: http://www.sciepub.com/journal/ajidm Editor-in-chief: Maysaa El Sayed Zaki
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American Journal of Infectious Diseases and Microbiology. 2016, 4(2), 44-51
DOI: 10.12691/ajidm-4-2-5
Open AccessArticle

Molecular Characterization of Polyomaviruses (BKV, JCV) in a Symptomatic Kidney Transplant Recipients in Sudan

Ibrahim A Helibi1, Hisham N Altayeb2, , Yousif F Hamedelni2, Khalid A Enan3 and Isam M Elkhidir4

1Department of Microbiology, Faculty of Medical laboratory, Omdurman Al ahlia University, Sudan

2Department of Microbiology, Faculty of Medical laboratory, Sudan University of Science and Technology, Sudan

3Department of Virology, Central Laboratory-The Ministry of Higher Education and Scientific Research, Sudan

4Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan

Pub. Date: May 03, 2016

Cite this paper:
Ibrahim A Helibi, Hisham N Altayeb, Yousif F Hamedelni, Khalid A Enan and Isam M Elkhidir. Molecular Characterization of Polyomaviruses (BKV, JCV) in a Symptomatic Kidney Transplant Recipients in Sudan. American Journal of Infectious Diseases and Microbiology. 2016; 4(2):44-51. doi: 10.12691/ajidm-4-2-5

Abstract

Background: The present study was to investigate the polyomaviruses (BKV, JCV) in asymptomatic kidney transplant recipients and to characterize the polyomaviruses circulating in Sudan. Methodology: A total of 100 plasma and 100urine samples were collected randomly from renal transplant recipients, attending Sudanese Renal Transplantation Society, at the period between September 2012 to March 2013, and subjected to polymerase chain reaction assay to detect viral DNA, urine cytology also done to identify viral inclusion (decoy cell), DNA sequencing was done for some gene to confirm the result, phylogenetic analysis of BKV, JCV based on T large gene were done MEGA 6 software. Results: Among the 100 renal transplant patients the polyomaviruses were detected in 3 urine specimens 3/100 (3%) by using urine cytology technique, that showed nuclear inclusions (decoy cells). And also detected in 32 urine specimens 32/100 (32%) while only 6/100 (6%) of virus nucleic acid had been present in their patients plasma, by using conventional PCR method. Successful sequences of BK, JCV virus on T large gene were done for 6 specimens from the virus isolate PCR products. Conclusions: This is the first report on molecular characterization that describes the circulation of polyomaviruses (BKV, JCV) in Sudan, Human polyomavirus is related to different clinical manifestations among renal transplant patients. The routine use of urine cytology and PCR on urine and plasma is a useful tool for the rapid and sensitive detection of reactivated BKV in asymptomatic recipients.

Keywords:
BK polyomavirus kidney transplant PCR Sudan

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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