American Journal of Infectious Diseases and Microbiology
ISSN (Print): 2328-4056 ISSN (Online): 2328-4064 Website: http://www.sciepub.com/journal/ajidm Editor-in-chief: Maysaa El Sayed Zaki
Open Access
Journal Browser
Go
American Journal of Infectious Diseases and Microbiology. 2013, 1(4), 70-74
DOI: 10.12691/ajidm-1-4-3
Open AccessArticle

Microbiological Analysis of Soil from Onu-Ebonyi Contaminated with Inorganic Fertilizer

Alo M.N.1, , Egbule U.C.C.2, Orji J.O.2 and Aneke C.J3

1Department of Biological Sciences, Faculty of Science and Technology, Federal University Ndufu Alike-Ikwo, Ebonyi, Nigeria

2Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, Abakaliki, Ebonyi, Nigeria

3Department of Biochemistry, Faculty of Applied Natural Sciences, Enugu State University of Science and Technology, Enugu, Nigeria

Pub. Date: July 04, 2013

Cite this paper:
Alo M.N., Egbule U.C.C., Orji J.O. and Aneke C.J. Microbiological Analysis of Soil from Onu-Ebonyi Contaminated with Inorganic Fertilizer. American Journal of Infectious Diseases and Microbiology. 2013; 1(4):70-74. doi: 10.12691/ajidm-1-4-3

Abstract

Microbiological analysis of soil from Onu-Ebonyi Izzi local government area of Ebonyi State, Nigeria contaminated with inorganic fertilizer was carried out with a view to determine the effect of the fertilizer on the microbial flora of the soil. Soil samples were analyzed for microbial burden. The result showed that the following organisms were isolated with their frequency of occurrence as follow: Pseudomonas species (33.3%) and Aspergillus species (54.4%) had the highest frequency of occurrence in the whole sample batches, while Streptococcus species had 6.0% and Geotrichum species (5.3%) had the least. Other predominant microorganism isolated include: Bacillus species, Staphylococcus species and Vibrio species, Escherichia species, Rhizopus species, Mucor species and Fusarium species. From the results, it could be concluded that the soil was contaminated and this could affect adversely the fertility of the soil.

Keywords:
soil bacteria fungi inorganic fertilizer Onu-Ebonyi

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Figures

Figure of 2

References:

[1]  Stewart, W.M., Dibb, D.W., Johnston, A.E., Smyth, T.J. The contribution of commercial fertilizer nutrients to food production. Agronomy Journal, 97: 1-6, 2005.
 
[2]  Abram, A., Forster, D.L. A primer on ammonia, nitrogen fertilizers, and natural gas markets. Department of Agricultural, Environmental, and Development Economics, Ohio State University. P. 38, 2005.
 
[3]  Lah, R. Soil carbon sequestration impact on global climate change and food security. Science Journal, 304(5677): 623-627, 2004.
 
[4]  Schinner, F., Sonnletner, R. Bodenokologie: Mikrobiologic und Bodenenzymatik. Springer Verlag, New York. P. 46, 1996.
 
[5]  Hyman, M.R., Kim, C.Y., Arp, D.J. Inhibition of ammonia monooxygenase in Nitromonas europaea by carbon disulfide. J. Bacteriol., 172: 4775-4782, 1990.
 
[6]  Khonje, D.J., Varsa, E.C., Klubek, B. The acidulation effects of nitrogenous fertilizers on selected chemical and microbiological properties of soil. Commun. Soil Sci. Plant Anal., 20: 1377-1395, 1989.
 
[7]  Godson, R.E.E., Mynepahs, K.S.C. The soil quality of new chemical fertilizer industry, Port Harcourt. Division of environmental health, University of Ibadan. PP. 7-37, 2004.
 
[8]  Parrett, J.F., Papendick, R.I., Horinck, S.B., Meyer, R.E. Soil quality attribute and relationship to alternative and sustainable agriculture. American Journal of Alternative Agriculture, 7: 5-11, 1992.
 
[9]  Reganold, J.P., Papendick, R.I., Parr, J.F. Sustainable agriculture. Scientific American, 262 (6): 112-120, 1990.
 
[10]  Glasson, J., Thervel, R., Chadrock, A. Introduction to environmental impact assessment, 2nd edition Mc-Graw hill, USA. PP. 3-6, 1999.
 
[11]  Ofomata, G.E.K. Nigeria in Maps (Eastern States). Ofomata Edition Ethiopia Publisher, Benin City. PP. 45-46, 1975.
 
[12]  Nduka, O. Environemental microbiology of aquatic and waste systems, 2nd edition. Springer Dordredd Heildelberg, London New York. PP. 157-168, 2011.
 
[13]  Onyeagba, R.A. Laboratory guide for microbiology. 1st edition. Crystal Publishers. No. 46 Owerri Road Okigwe, Imo State Nigeria. PP. 95-117, 2004.
 
[14]  Cheesbrough, M. District laboratory practice in tropical countries, part 2. Cambridge University Press, Cambridge, UK. PP. 137-150, 2006.
 
[15]  Ezeonu, I.M., Okafor, J.I., Ogbonna, J.C. Laboratory exercises in microbiology. A practical manual for students of tertiary institution. 1st edition. Ephrata Printing and Publishing Company, N0. 12 Orba Road, Nsukka, Nigeria. PP. 67-69, 2011.
 
[16]  Hashem, A.R. Influence of crude oil contamination on the chemical and microbiological aspects of Saudi Arabian soil. J. King Saud. Univ., 8(1): 11-18, 1996.
 
[17]  Barkay, T., Pritchard, H. Adaptation of aquatic microbial communities to pollutant stress. Microbiol. Sci., 5: 165-669, 1988.
 
[18]  Kivisaar, M. Stationary mutagenesis: Mechanisms that accelerate adaptation of microbial population under environmental stress. Environ. Microbial., 5 (10): 814-827, 2003.
 
[19]  Obahiagbon, K.O., Erhabor, I. Biodegradation of refinery wastewater using Pseudomonas aeruginosa and Fusarium. National Journal of Information Sharing and initiative, 1: 77-111, 2002.
 
[20]  Lovely, D.R., Philip, E.J.P., Gorby, Y.A., Linda, E.R. Microbial reduction of uranium. Nature, 350: 413-416, 1991.
 
[21]  Al-Yemeni, M.N., Hashem, A.R. Heavy Metals and Microbial Analysis of Soil Samples Collected from Aramco Gulf Operations Company Al-khafji, (AGOC) Saudi Arabia. Saudi Journal of Biological Sciences, 13(2): 129-133, 2006.
 
[22]  Okey, C. Ecology and genomics of Bacillus subtilis. Trends in Microbiology, 16 (6): 268-274, 2003.
 
[23]  Johnson, M.S., Eaton, J.W. Environmental contamination through residual trace metals dispersed from dielectric lead-zinc mine. Journal of Envron. Qual., 9 (2): 175-179, 1980
 
[24]  Dobler, R., Burri, P., Gruiz, K., Brandl, H., Bachofen, R. Variability in microbial population in soil highly polluted with heavy metals in the basis of substrate utilization pattern analysis. J. soils and Sed., 1 (3): 151-158, 2001.
 
[25]  Giller, K.E., Witter, E., McGrath, S.P. Toxicity of heavy metals to microorganism and microbial processes in agricultural soil: A review. Journal of Soil Biology and Biochemistry, 30: 1389-1414, 1998.
 
[26]  Huang, C., Khan, R.S. Effect of cadmium, lead and their interaction on the size of microbial biomass in a red soil. Journal of Soil Environment, 1: 227-236, 1998.
 
[27]  Harris, J.A., Benthan, H., Birch, P. Soil microbial community provide index to progress direction of restoration. Journal of Restoration Management Notes, 9: 133-135, 1991.
 
[28]  Depue, I. New Jersey zinc/mobile chemical site. Sample Result Fact Sheet, 1: 38-41, 1992.
 
[29]  Pockock, S.J., Smith, M., Baghurst, P. Environmental lead and children’s intelligence: A systematic review of the epidemiological evidence. Brit. Med. J., 309: 1189-1197, 1994.