American Journal of Infectious Diseases and Microbiology
ISSN (Print): 2328-4056 ISSN (Online): 2328-4064 Website: Editor-in-chief: Maysaa El Sayed Zaki
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American Journal of Infectious Diseases and Microbiology. 2015, 3(5), 141-143
DOI: 10.12691/ajidm-3-5-1
Open AccessArticle

Occurrence of a Cholera Outbreak in Central India

Meena Mishra1, Arvind Kurhade2, Yagnesh Thakar3, Geeta Kurhade4, Angel Justiz Vaillant5, , Sudhir Lakhdive6, Tejaswini H Joshi7 and Sehlule Vuma2

1Department of Microbiology, Government Medical College and Hospital, Nagpur, India

2Pathology and Microbiology Unit, Department of Para,Clinical sciences, University of West Indies, St. Augustine, Trinidad and Tobago

3Consultant Microbiologist, Nagpur, India

4Physiology Unit, Department of Preclinical Sciences, University of West Indies, St. Augustine, Trinidad and Tobago

5Pathology and Microbiology Unit, Department of Para Clinical sciences, UWI, St. Augustine, Trinidad and Tobago

6Professor of Surgery, Great Eastern Medical School, Srikakulum, India

7Post Graduate Diploma Program, Defense Services Staff College, Madras University, Wellington, India

Pub. Date: November 17, 2015

Cite this paper:
Meena Mishra, Arvind Kurhade, Yagnesh Thakar, Geeta Kurhade, Angel Justiz Vaillant, Sudhir Lakhdive, Tejaswini H Joshi and Sehlule Vuma. Occurrence of a Cholera Outbreak in Central India. American Journal of Infectious Diseases and Microbiology. 2015; 3(5):141-143. doi: 10.12691/ajidm-3-5-1


The aim of this research was to carry out a bacteriological study of Vibrio cholerae in Central India. Cholera is an important public health problem in India and it continues to be a major concern as it is an important cause of morbidity mortality. A total of 44 strains of V. cholerae were isolated from 150 stool samples received from patients with acute diarrhea. All samples were plated onto different bacteriological media. Biotyping was performed as per the standard procedures. Confirmation of the strains was done by seroagglutination using Polyvalent O1, monospecific Ogawa and Inaba antisera. Antibiotic susceptibility testing was performed by Kirby Bauer's disk diffusion method. The majority of the isolates belonged to type 27 (70.45%, i.e. 31 isolates). All Isolates were susceptible to tetracycline, norfloxacin and were relatively susceptible to gentamicin (95%) and chloramphenicol (95%). Continued monitoring, surveillance of all outbreaks and notification to relevant authorities are of utmost importance in the fight against cholera. In addition, the molecular subtyping was essential to improve the tracing of the sources of the outbreak.

Vibrio cholerae bacteria bacteriological media Antibiotic susceptibility India

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