American Journal of Infectious Diseases and Microbiology
ISSN (Print): 2328-4056 ISSN (Online): 2328-4064 Website: http://www.sciepub.com/journal/ajidm Editor-in-chief: Maysaa El Sayed Zaki
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American Journal of Infectious Diseases and Microbiology. 2015, 3(3), 112-124
DOI: 10.12691/ajidm-3-3-4
Open AccessArticle

Isolation and Characterization of Microbial Contamination from Computer Accessories used in Different Department of Hazara University and Diagnostic Laboratories of District Mansehra, Pakistan

Zeba Gul Burki1, Mukhtiar Hassan2, Faiza Naseer3, , Sohail Ahmad4, Samiullah Burki5, Mohammad Saleem6, Attiya Nazish7 and Shafiq-ur- Rehman7

1Department of Microbiology, Hazara University, Mansehra

2Department of Microbiology, Hazara University, Mansehra, Pakistan

3College of Pharmacy, Govt College, University, Faisalabad

4Department of Biochemistry, Hazara University, Mansehra

5Department of pharmacology, faculty pharmacy, Federal Urdu University of Arts, Science and Technology, Pakistan

6College of Pharmacy, Punjab University, Lahore, Pakistan

7College of Pharmacy, Govt College, University, Faisalabad, Pakistan

Pub. Date: May 22, 2015

Cite this paper:
Zeba Gul Burki, Mukhtiar Hassan, Faiza Naseer, Sohail Ahmad, Samiullah Burki, Mohammad Saleem, Attiya Nazish and Shafiq-ur- Rehman. Isolation and Characterization of Microbial Contamination from Computer Accessories used in Different Department of Hazara University and Diagnostic Laboratories of District Mansehra, Pakistan. American Journal of Infectious Diseases and Microbiology. 2015; 3(3):112-124. doi: 10.12691/ajidm-3-3-4

Abstract

150 samples were collected from computer accessories used in Hazara University and different diagnostic laboratories of Mansehra, examined for the total bacterial count and maximum growths were observed. Samples were analyzed for further identification of micro-organisms such as E.coli, Klebsiella, Staph. aureus and Staph. epidermidis. These organisms were detected in the percentage of 46.66 of E. coli, 20% of Klebsiella, 16.66% of S. aureus & 16.66% of S. Epidermidis and identified on selective media, i.e. EMB agar and Mannitol salt agar. Furthermore, biochemical tests, including IMVIC Test, Catalase Test and Coagulase Tests were performed to confirm the presence of micro-organisms and their susceptibility also checked against different standard antibiotics and their zone of inhibitions were measured and noticed. E.coli showed maximum resistance of 97.36% against Erythromycin, Klebsiella showed against Amoxil + Clavolunic acid about 83.83%, Staph aureus showed against Erythromycin about 64.64% and Staph. epidermidis resistance was 90.9% against Erythromycin and Gentamycin. E.coli and Klebsiella showed maximum sensitivity for Meropenem 67.22% and 72.72% respectively while Staph. aureus and Staph. epidermidis maximum sensitivity for Vancomycin about 82.82% and 72.72% respectively. These results indicate that the computer accessories might act as environmental vehicles for the transmission of potentially pathogenic bacteria in our surroundings and also indicate the need for increasing awareness among computer users on cleaning of such surfaces or disinfection and adequate hand-washing hygiene.

Keywords:
E.coli Klebsiella Staph. aureus Staph. epidermidis Computer accessories EMB agar Mannitol salt agar Antibiotic resistance

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