Isolation and Characterization of Microbial Contamination from Computer Accessories used in Different Department of Hazara University and Diagnostic Laboratories of District Mansehra, Pakistan

Zeba Gul Burki¹, Mukhtiar Hassan², Faiza Naseer^3, , Sohail Ahmad⁴, Samiullah Burki⁵, Mohammad Saleem⁶, Attiya Nazish⁷ and Shafiq-ur- Rehman⁷

¹Department of Microbiology, Hazara University, Mansehra

²Department of Microbiology, Hazara University, Mansehra, Pakistan

³College of Pharmacy, Govt College, University, Faisalabad

⁴Department of Biochemistry, Hazara University, Mansehra

⁵Department of pharmacology, faculty pharmacy, Federal Urdu University of Arts, Science and Technology, Pakistan

⁶College of Pharmacy, Punjab University, Lahore, Pakistan

⁷College of Pharmacy, Govt College, University, Faisalabad, Pakistan

Cite this paper:
Zeba Gul Burki, Mukhtiar Hassan, Faiza Naseer, Sohail Ahmad, Samiullah Burki, Mohammad Saleem, Attiya Nazish and Shafiq-ur- Rehman. Isolation and Characterization of Microbial Contamination from Computer Accessories used in Different Department of Hazara University and Diagnostic Laboratories of District Mansehra, Pakistan. American Journal of Infectious Diseases and Microbiology. 2015; 3(3):112-124. doi: 10.12691/ajidm-3-3-4

Abstract

150 samples were collected from computer accessories used in Hazara University and different diagnostic laboratories of Mansehra, examined for the total bacterial count and maximum growths were observed. Samples were analyzed for further identification of micro-organisms such as E.coli, Klebsiella, Staph. aureus and Staph. epidermidis. These organisms were detected in the percentage of 46.66 of E. coli, 20% of Klebsiella, 16.66% of S. aureus & 16.66% of S. Epidermidis and identified on selective media, i.e. EMB agar and Mannitol salt agar. Furthermore, biochemical tests, including IMVIC Test, Catalase Test and Coagulase Tests were performed to confirm the presence of micro-organisms and their susceptibility also checked against different standard antibiotics and their zone of inhibitions were measured and noticed. E.coli showed maximum resistance of 97.36% against Erythromycin, Klebsiella showed against Amoxil + Clavolunic acid about 83.83%, Staph aureus showed against Erythromycin about 64.64% and Staph. epidermidis resistance was 90.9% against Erythromycin and Gentamycin. E.coli and Klebsiella showed maximum sensitivity for Meropenem 67.22% and 72.72% respectively while Staph. aureus and Staph. epidermidis maximum sensitivity for Vancomycin about 82.82% and 72.72% respectively. These results indicate that the computer accessories might act as environmental vehicles for the transmission of potentially pathogenic bacteria in our surroundings and also indicate the need for increasing awareness among computer users on cleaning of such surfaces or disinfection and adequate hand-washing hygiene.

Keywords:
E.coli Klebsiella Staph. aureus Staph. epidermidis Computer accessories EMB agar Mannitol salt agar Antibiotic resistance

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References:

[1]	Ali WS, Alkhezali KA, Taha BM. Bacterial Contamination of Computer Keyboards and Mice in a University Setting. Journal of Biology, Agriculture and Healthcare. 2013; 3(18): 11-15.
[2]	Anastasiades P, Pratt TL, Rousseau LH, Steinberg WH, Joubert G. Staphylococcus aureus on computer mice and keyboards in intensive care units of the university’s academic hospital, Bloemfontein and ICU staff’s knowledge of its hazards and cleaning practices. South African Journal of Epidemiology and Infection. 2009; 24(20): 22-26.
[3]	Bhalla A, Aron D, Donskey C. Staphylococcus aureus intestinal colonization is associated with increased frequency of S. aureus on skin of hospitalized patients. BMC Infectious Diseases. 2007; 7: 105-110.
[4]	Bures S, Fishbain JT, Uyehara CF, Parker JM, Berg BW. Computer keyboards and faucet handles as reservoirs of nosocomial pathogens in the intensive care unit. American Journal of Infection and Control. 2000; 28(6): 465-471.
[5]	Cogen AL, Nizet V, Gallo RL. Skin microbiota: a source of disease or defence. British Journal of Dermatology. 2008; 158(3): 442-455.
[6]	David MZ, Daum RS. Community-Associated Methicillin-Resistant Staphylococcus aureus: Epidemiology and Clinical Consequences of an Emerging Epidemic. Clinical Microbiology Review. 2010; 23(3): 616-687.
[7]	Ejaz H, Haq I, Zafar A, Mahmood S, Javed MM. Urinary tract infections caused by extended spectrum β-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumonia. African Journal of Biotechnology. 2011; 10(73): 16661-16666.
[8]	Enemuor SC, Apeh TA, Oguntibeju O. Microorganisms associated with computer keyboards and mice in a university environment. African Journal of Microbiology Research. 2012; 6(20): 4424-4426.
[9]	Forbes BA, Sahm DF, Weissfeld AS. Bailey and Scott’s Diagnostic Microbiology.12th edition. Mosby Elsevier, China. 2007.
[10]	Grice EA, Kong HH, Conlan S, Deming CB, Davis J, Young AC. Topographical and Temporal Diversity of the Human Skin microbiome. Science. 2009; 324(5931): 1190-1192.
[11]	Itah AY, Ben AE. Incidence of enteric bacteria and Staphylococcus aureus in day care centers in Akwa-Ibom State, Nigeria. Southeast Asian Journal of Tropical Medicine and Public Health. 2004; 35(1): 202-209.
[12]	Kacmaz B, Sultan N. In vitro susceptibilities of Escherichia coli and Klebsiella spp. To ampicillin-sulbactam and amoxicillin-clavulanic acid. Japanese Journal of infectious diseases. 2007; 60(4): 227-229.
[13]	Koneman EW. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 2006; 6: 38-46.
[14]	Krausse R, Piening K, Ullmann U. Inhibitory effects of various micro-organisms on the growth of Helicobacter pylori. Letter in Applied Microbiology. 2005; 40(1): 81-86.
[15]	Mehdinejad M, Sheikh AF, Jolodar A. Study of methicillin resistance in staphylococcus aureus and species of coagulase negative staphylococci isolated from various clinical specimens. Pakistan Journal of Medical Sciences. 2008; (24)5: 719-724.
[16]	Oluduro AO, Ubani EK, Ofoezie, IE. Bacterial assessment of electronic hardware user interfaces in Ile-Ife, Nigeria. Journal of Basic Applied Pharmaceutical Sciences. 2011; 32(3), 323-334.
[17]	Qian Q, Eichelberger K, Kirby JE. Rapid Identification of Staphylococcus aureus in Blood Cultures by Use of the Direct Tube Coagulase Test. Journal of Clinical Microbiolgy. 2007; 45(7): 2267-2269.
[18]	Rutala WA, White MS, Gergen MF, Weber DJ. Bacterial Contamination of Keyboards: Efficacy and Functional Impact of Disinfectants. Infection Control and Hospital Epidemiology. 2006; 27(4): 372-377.
[19]	Tagoe D, Kumi-Ansah F. Computer keyboard and mice: Potential sources of disease transmission and infection. International Journal of Public Health. 2011; 1(2): 5611-5619.
[20]	Tenover FC. Mechanisms of antimicrobial resistance in bacteria. American Journal of Infection and Control. 2006; 34(5): 64-73.
[21]	Winn W, Allen S, Janda W, Koneman E, Procop G, Schreckenberger P, Woods G. Color atlas and textbook of diagnostic microbiology. Lippincott Williams & Wilkins, Philadelphia, PA. 2006; 6th edition.
[22]	Zahera M, Rastogi C, Singh P, Iram S, Khalid S, Kushwaha A. Isolation, Identification and Characterization of Escherichia Coli from Urine Samples and their Antibiotic Sensitivity Pattern. European Journal of Experimental Biology. 2011; 1(2): 118-124.