American Journal of Infectious Diseases and Microbiology
ISSN (Print): 2328-4056 ISSN (Online): 2328-4064 Website: Editor-in-chief: Maysaa El Sayed Zaki
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American Journal of Infectious Diseases and Microbiology. 2015, 3(3), 91-94
DOI: 10.12691/ajidm-3-3-1
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Topoisomerases II Mutations in Ciprofloxacin-resistant Clinical Isolates of Pseudomonas aeruginosa in Makassar, Indonesia

Irene E.Rieuwpassa1, Ressy Dwiyanti2, 3, Yadi Yasir4, 3, Muhammad Sabir2, 3 and Mochammad Hatta3,

1Department of Oral Biology, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia

2Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia

3Department of Microbiology, Molecular Biology and Immunology Laboratory, Facculty of Mediciene, Hasanuddin University, Makassar, Indonesia

4Department Microbiology and Immunology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia

Pub. Date: May 07, 2015

Cite this paper:
Irene E.Rieuwpassa, Ressy Dwiyanti, Yadi Yasir, Muhammad Sabir and Mochammad Hatta. Topoisomerases II Mutations in Ciprofloxacin-resistant Clinical Isolates of Pseudomonas aeruginosa in Makassar, Indonesia. American Journal of Infectious Diseases and Microbiology. 2015; 3(3):91-94. doi: 10.12691/ajidm-3-3-1


Background: Ciprofloxacin constitute a clinically successful and widely used class of broad-spectrum antibiotics; however, the emergence and spread of resistance increasingly limits the use of this fluoroquinolone in the treatment and management of microbial disease. Ciprofloxacin is the most frequently used member of the fluoroquinolones during initial eradication therapy of Pseudomonas aeruginosa. The emergence of ciprofloxacin-resistant Pseudomonas aeruginosa (P. aeruginosa) has been reported. In this study, we examined mutations in the quinolone resistance-determining regions of the topoisomerase II (GyrA) genes of 11 clinical isolates of P. aeruginosa that resistance to ciprofloxacin. Methods: In this study we examined the relationship between gene mutations of topoisomerase II (gyrA) with ciprofloxacin resistance of P. aeruginosa. The examination performed by disc diffusion test followed by RFLP-PCR. Results: The results of disc diffusion test showed that 13 samples were sensitive to ciprofloxacin, 11 samples were resistant to ciprofloxacin. In this study we found mutation in GyrA in 6 (54.5%) of ciprofloxacin resistant samples. There was no mutation found in ciprofloxacin sensitive samples. Conclusion: Mutation in topoisomerases genes are the main mechanism of fluoroquinolone resistance in P. aeruginosa.

Topoisomerase II (GyrA) Pseudomonas aeruginosa mutation PCR-RFLP

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