American Journal of Infectious Diseases and Microbiology
ISSN (Print): 2328-4056 ISSN (Online): 2328-4064 Website: http://www.sciepub.com/journal/ajidm Editor-in-chief: Maysaa El Sayed Zaki
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American Journal of Infectious Diseases and Microbiology. 2015, 3(1), 32-37
DOI: 10.12691/ajidm-3-1-5
Open AccessArticle

Epidemiologicl and Genetic Studies of Enterotoxigenic Staphylococcus aureus Isolated from Goat and Human

Nashwa O. Khalifa1, , Fatma I. Elhofy2, Hanan A. Fahmy3 and A. M. A. Barakat4

1Zoonoses Department, Faculty of Veterinary Medicine, Benha University, Toukh, 13736, Egypt

2Bacteriology, Immunologyand Mycology Department, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt

3Biotechnology Department, Animal Health Research Institute (AHRI), Giza 11331, Egypt

4Zoonoses Department, National Research Center, Dokki, 12622, Giza, Egypt

Pub. Date: February 13, 2015

Cite this paper:
Nashwa O. Khalifa, Fatma I. Elhofy, Hanan A. Fahmy and A. M. A. Barakat. Epidemiologicl and Genetic Studies of Enterotoxigenic Staphylococcus aureus Isolated from Goat and Human. American Journal of Infectious Diseases and Microbiology. 2015; 3(1):32-37. doi: 10.12691/ajidm-3-1-5

Abstract

The objective of the present study was to investigate the epidemiological and genetic relationships of classical enterotoxins of S. aureus in goat’s raw milk, meat and food handlers in Toukh city in Qaluobia governorate, Egypt. A total of 100 goat, s raw milk and meat samples (50 of each) were collected from randomly distributed herds in streets for buying milk and in public markets for peddler meat. Hand and nasal swabs were collected from milkers and butchers (30 of ech). All samples were subjected for bacteriological examination for isolation and identification of S. aureus. Isolates were underwent reversed passive latex agglutination technique for detection of enterotoxigenic S. aureus. A multiplex PCR assay could successfully amplify the diagnostic DNA bands of 270bp, 165bp, 69bp and 306bp of genes for staphylococcal enterotoxins A, B, C, and D respectively. PCR was applied on the serologically identified 16 (20.25%) isolates out of 79 S. aureus which isolated from the examined goat’s food samples and human handlers by using one universal forward and reverse primers, specific for each individual toxin gene. None of the samples was positive for SEE indicating the zoonotic and genetic relationships.

Keywords:
S. aureus latex agglutination multiplex PCR enterotoxigenic gene

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References:

[1]  Valle, J. Gomez-Lucia, E., Piriz, S. Goyache, J., Orden, J. A. and Vadillo, S. Enterotoxin production by staphylococci isolated from healthy goats. Applied and Environmental Microbiology, 1990, 56 (5):1323-1326.
 
[2]  Akineden, O., Hassan, A. A., Schneider, E., Usleber, E. Enterotoxigenic properties of Staphylococcus aureus isolated from goat's milk cheese. Int. J. Food Microbiol. 2008, 124: 211-216.
 
[3]  Bayles, K. W. and Landolos. J. J. Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D. J. Bacteriol., 1989, 171: 4799-4806.
 
[4]  Sharma, N. K., Rees, C. E. D. and Dodd, C. E. Development of a Single-Reaction Multiplex PCR Toxin Typing Assay for Staphylococcus aureus Strains. Applied and Environmental Mirobiology, 2002, 66 (4): 1347-1353.
 
[5]  Jaradat, Z. W., Tarazi, Y. H., and Ababneh, Q. O. Molecular characterization of Staphylococcus aureus isolated from meat and their antibiotic resistance profiles. Pak. Vet. J., 2014, 34 (1): 58-62.
 
[6]  Morandi, S., Brasca, M., Andrighetto, C., Lombardi, A. and Lodi, R. Phenotypic and genotypic characterization of Staphylococcus aureus strains from Italian dairy Pproducts. International Journal of Microbiology, 2009,: 1-7.
 
[7]  Wei, H. L. and Chiou, C. S. Molecular subtyping of Staphylococcus aureus from an outbreak associated with a food handler. Epidemiol. Infect., 2002, 128: 15-20.
 
[8]  El-Jakee, J. K., Aref, N. E., Gomaa, A., El-Hariri, M. D., Galal, H. M., Sherif A., Omar, S. A. and Samir, A. Emerging of coagulase negative staphylococci as a cause of mastitis in dairy animals: An environmental hazard. International Journal of Veterinary Science and Medicine, 2013, 1:74-78.
 
[9]  Schmitt, M., Schuler-Schmid, U. and Scmidt-Lorenz, W. Temperature limits of growth, TNase, and enterotoxin production of Staphylococcus aureus strains isolated from foods. Int. J. Food Microbiol., 1990, 11: 1-19.
 
[10]  Rahimi, E. Detection of classical enterotoxins of Staphylococcus aureus in raw sheep, goat, camel, and water buffalo milk by ELISA method. , 2013, 22 (2): 181-184.
 
[11]  Normanno, G., La Salandra, G., Dambrosio, A., Quaglia, N. C., Corrente, M., Parisi, A., Santagada, G. Firinu, A., Crisetti, E. and Celano, G. V. Occurrence, characterization and antimicrobial resistance of enterotoxigenic Staphylococcus aureus isolated from meat and dairy products. International Journal of Food Microbiology, 2007, 115 (3): 290-296.
 
[12]  Presscott, L. M., Harley, J. P. and Klein, D. A. Text book of Microbiology. Brown Publishers. 5th ed., 2002,: 441-442.
 
[13]  Dorgham, S. M., Hamza, D. A. Khairy, E. A. and Hedia, R. H. Methicillin-resistant staphylococci in mastitic animals in Egypt. Global Veterinaria, 2013, 11 (6): 714-720.
 
[14]  El-Shenawy, M., El-Hosseiny L., Tawfeek, M., El-Shenawy, M., Baghdadi, H., Ola Saleh, Mañes, J. and Soriano, J. M. Nasal carriage of enterotoxigenic Staphylococcus aureus and risk factors among food handlers-Egypt. Food and Public Health, 2013, 3 (6): 284-288.
 
[15]  El-Shenawy, M., Tawfeek, M., El-Hosseiny, L., El-Shenawy, M., Farag, N., Baghdadi, H., O Saleh, O., Mañes, J. and Soriano, J. M. Cross sectional study of skin carriage and enterotoxigenicity of Staphylococcus aureus among food handlers. Open Journal of Medical Microbiology, 2014, 4: 16-22.
 
[16]  Scott, E. Food safety and foodborne diseases in 21st century homes. Can. J. Infect. Dis., 2003, 14: 277-280.
 
[17]  Argudin, M. A., Mendoza, M. C., and Rodicio, M. R. Food poisoning and Staphylococcus aureus enterotoxins. Toxins, 2010, 2: 1751-1773.
 
[18]  Balaban, N. and Rasooly, A. Staphylococcal enterotoxins. Int. J. Food Microbiol., 2000, 61: 1-10.
 
[19]  Forbes, B. A., Sahm, D. F.and Weissfeld A. S.. Baily and Scott’s Diagnostic Microbiology. 2007, 12th ed. St. Louis, Missouri: Mosby, Elsevier.
 
[20]  Cruickshank, R. J. P., Duguid, B. P. Marmion and Swain, R. H.. The Practice of Medical Microbioogy. 1975, 12th ed. vol. 11, Edinburgh, London and New York.
 
[21]  Thaker, H. C., Brahmbhatt, M. N. and Nayak, J. B. Isolation and identification of Staphylococcus aureus from milk and milk products and their drug resistance patterns in Anand, Gujarat. Vet. World, 2013, 6 (1): 10-13.
 
[22]  Zahoor S. and Bhatia A. Bacteria: Silent Killers in Food Science Reporter, 2007,: 33-34.
 
[23]  Rea, M., O'connor, F., Daly, C. and O'regan, W. Biochemical characteristics and enterotoxigenicity of S. aureus isolates from bovine mastitis. Irish J. Food Science and Technology, 1980, 4 (1): 45-55.
 
[24]  Saeed, H. A. and Hamid, H. H. Bacteriological and parasitological assessment of food handlers in the Omdurman Area of Sudan. J.Microbiol. Immunol. Infect., 2010, 43 (1): 70-73.
 
[25]  Andargie, G., Kassu, A., Moges, F., Tiruneh, M. and Huruy, K. Prevalence of bacteria and intestinal parasites among food-handlers in Gondar Town, Northwest Ethiopia. Journal of Health, Population and Nutrition, 2008, 26: 451-455.
 
[26]  Loeto, D., Matsheka, M. I. and Gashe, B. A. Enterotoxigenic and antibiotic resistance determination of Staphylococcus aureus strains isolated from food handlers in Gaborone, Botswana. Journal of Food Protection, 2007, 70: 2764-2768.
 
[27]  Soriano, J. M., Font, G., Molto, J. C. and Males, J. Enterotoxigenic staphylococci and their toxins in restaurant foods. Trends in Food Science and Technology, 2002, 13: 60-67.
 
[28]  Bhatia A. and Zahoor S. Staphylococcus aureus Enterotoxins: A Review. Journal of Clinical and Diagnostic Research, 2007, 3:188-197.
 
[29]  Pinchuk, I. V., Beswick, E. J. and Reyes, V. E. Staphylococcal enterotoxins. Toxins, 2010, 2: 2177-2197.
 
[30]  Udo, E. E., Al-Bustan, M. A., Jacob, L. E. and Chugh, T. D. Enterotoxin production by coagulase-negative staphylococci in restaurant workers from Kuwait City may be a potential cause of food poisoning. J. Med. Microbiol., 1999, 48: 819-823.
 
[31]  Gutiérrez, D., Delgado, S., Vázquez-Sánchez, D., Martínez, B., López Cabo, M., Rodríguez, A., Herrera, J. J. and Garcíaa, P. Incidence of Staphylococcus aureus and analysis of associated bacterial communities on food industry surfaces. Applied and Environmental Microbiology, 2012, 78 (24): 8547-8554.
 
[32]  Le Loir, Y., Baron, F. and Gautier M. Staphylococcus aureus and food poisoning. Genet. Mol. Res., 2003, 2: 63-76.
 
[33]  Humphreys, H., Keane, C. T., Hone, R., Pomeroy, H., Russell, R. J., Arbuthnott, J. P. and Coleman, D. C. Enterotoxin production by Staphylococcus aureus isolates from cases of septicemia and from healthy carriers. J. Med. Microbiol., 1989, 28: 163-172.
 
[34]  Collery, M. M., Smyth, D. S., Twohig, J. M., Shore, A. C., Coleman, D. C. and Smyth, C. J. Molecular typing of nasal carriage isolates of Staphylococcus aureus from an Irish university student population based on toxin gene PCR, agr locus types and multiple locus, variable number tandem repeat analysis. Journal of Medical Microbiology, 2008, 57:348-358.
 
[35]  Becker, K., Friedrich, A. W., Peters, G. and Von Eiff, C. Systematic survey on the prevalence of genes coding for staphylococcal enterotoxins SElM, SElO, and SElN. Mol. Nutr. Food Res., 2004, 48:488-495.
 
[36]  Omoe, K., Hu, D.-L., Takahashi-Omoe, H., Nakane, A. and Shinagawa, K. Comprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates. FEMS. Microbiol. Lett., 2005, 246: 191-198.
 
[37]  Boerema, J. A., Clemens, R. and Brightwell, G. Evaluation of molecular methods to determine enterotoxigenic status and molecular genotype of bovine, ovine, human and food isolates of Staphylococcus aureus. Int. J. Food Microbiol.,2006, 107, 192-201.
 
[38]  Bania, J., Dabrowska, A., Korzekwa, K., Zarczynska, A., Bystron, J., Chrzanowska, J. and Molenda, J. The profiles of enterotoxin genes in Staphylococcus aureus from nasal carriers. Lett. Appl. Microbiol., 2006, 42, 315-320.
 
[39]  Nashev, D., Toshkova, K., Bizeva, L., Akineden, O¨., La¨ mmler, C. and Zscho¨ ck, M. Distribution of enterotoxin genes among carriage-and infection-associated isolates of Staphylococcus aureus. Lett. Appl. Microbiol., 2007, 45: 681-685.
 
[40]  Haeghebaert, S., Le Querrec, F., Gallay, A., Bouvet, P., Gomez, M. and Vaillant V. Les toxi-infections alimentaires collectives en France, en 1999 et 2000. Bull. Epidemiol. Hebdo, 2002, 23: 105-109.
 
[41]  Betley, M., and J. J. Mekalanos. Nucleotide sequence of type A staphylococcal enterotoxin gene. J. Bacteriol., 1988, 170: 34-41.
 
[42]  Jones, C. L. and Khan, S. A. Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus. J. Bacteriol., 1986, 166:29-33.
 
[43]  Bohach, G. A., and Schlievert.P. M. Nucleotide sequence of the staphylococcal enterotoxin C1 gene and relatedness to other pyrogenic toxins. Mol. Gen. Genet.,1987, 209:15-20.
 
[44]  Couch, J. L., Soltis, M. T. and Betley,. M. J. Cloning and nucleotide sequence of the type E staphylococcal enterotoxin gene. J. Bacteriol., 1988, 170: 2954-2960.