American Journal of Infectious Diseases and Microbiology
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American Journal of Infectious Diseases and Microbiology. 2014, 2(6), 138-144
DOI: 10.12691/ajidm-2-6-1
Open AccessArticle

Antibiotics Resistance of Bacteria Associated with Pneumonia in HIV/AIDS Patients in Nigeria

Ojo- Bola. O1, and Oluyege A.O2

1Medical Microbiology Departments, Federal Medical Centre, Ido-Ekiti, Ekiti State, Nigeria

2Department of Microbiology, Ekiti State University, Ado –Ekiti, Ekiti State, Nigeria

Pub. Date: November 20, 2014

Cite this paper:
Ojo- Bola. O and Oluyege A.O. Antibiotics Resistance of Bacteria Associated with Pneumonia in HIV/AIDS Patients in Nigeria. American Journal of Infectious Diseases and Microbiology. 2014; 2(6):138-144. doi: 10.12691/ajidm-2-6-1


This study was conducted to determine the antimicrobial resistance of bacteria associated with pneumonia in HIV/AIDS and HIV negative patients attending Federal Medical Centre, Ido-Ekiti, Ekiti State. A total of 300 sputum samples were collected (180 from HIV/AIDS patients and 120 samples from HIV negative patients diagnosed for pneumonia) and were selected by random sampling. The sputum samples were collected and examined for bacteria using microscopic, cultural and biochemical characteristics. Antibiogram was carried out by disc diffusion method. Results showed that male subjects with HIV/AIDS were more susceptible to infection by bacteria associated with pneumonia than females. The age group of 31-40 years and 71-80 years had the highest occurrence of bacterial pneumonia in HIV/AIDS and HIV negative populations respectively. The prevalence of pneumonia in HIV/AIDS and HIV negative patients was 55.6% and 43.3% respectively. A variety of bacteria was isolated in both populations with Escherichia coli (40%) predominating in HIV/AIDS patients followed by Pseudomonas aeruginosa (35%), S. aureus (20%) and the least was Klebsiella pneumoniae (5%). K. pneumoniae (44.2%) was the predominant bacterium in HIV-negative patients, followed by Streptococcus pneumoniae (30.8%) and S. aureus (25.0%) These bacterial isolates were tested for resistance to twenty antibiotics prescribed in hospitals. However, resistance to antibiotics ranged between 52.5% to 100% in HIV/AIDS patients and 18.8% to 84.6% in HIV negative patients. Multiple antibiotic resistance to nine classes of these twenty antibiotics was observed in 53.0% and 19.2% of all organisms isolated from HIV/AIDS group and HIV negative patients respectively. However, the variation in the profile of bacterial organism isolated in both populations was statistically significant; suggesting that immune status of HIV/AIDS patients predisposed them to infection by some of the bacteria.

antibiotics bacteria HIV/AIDS pneumonia Nigeria

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[1]  Vray, M., Hecht, F.M. and Picker, L.J. (2008). Clinical features and etiology of pneumonia in acid-fast bacillus sputum smear-negative HIV-n infected patients hospitalized in Asia and Africa. AIDS 22: 1323-1332
[2]  Jaspan, H.B., Huang, L.C., Cotton, M.F., Whitelaw, A. and Myer, L. (2008). Bacterial Disease and Antimicrobial Susceptibility Patterns in HIV-Infected, Hospitalized Children: A Retrospective Cohort Study. PLoS ONE 3(9): 3260.
[3]  Bekele, A. and Bethany, G. (2009). Bacterial Pneumonia in Hospitalized Patients With HIV Infection. The Pulmonary Complications, ICU Support, and Prognostic Factors of Hospitalized Patients With HIV (PIP) Study.American Rev Respiratory Disease 148:1523-1529.
[4]  Adeleye, A., Uju, L., Idika, N. and Sobande, O. (2008). Cotrimoxazole resistance in Streptococcus pneumoniae isolated from sputum of HIV-positive patients. West Indian medical Journal 57 no.5.
[5]  Rano, A., Agusti, C., Sibila, O., Torres, A.(2005). Pulmonary infections in non-HIV-immunocompromised patients. Curr Opin Pulmonary Medical 11 (3): 213-217. WHO (2008). "Causes of death in neonates and children under five in the world 2004".
[6]  Cordero, E. Pachón, J., Rivero, A., Girón-González, J., Gómez-Mateos, M., Merino,M. Torres-Tortosa, M. González-Serrano, L. and Collado, A. (2002). Usefulness of sputum culture for diagnosis of bacterial pneumonia in HIV-infected patients. European Journal of Clinical Microbiology and Infectious Diseases. Volume 21, number 5, 362-367.
[7]  Ochei, J. and Kolkhtar, A. (2000). Bacteriology: Medical Laboratory Science, theory and practice. In:Bulakh P.M. and Deshmukh S. (eds). Tata McGraw-Hill publishing Company limited New Delhi. Pp. 525-752.
[8]  Cheesbrough, M. (2000). Microbiological test: District Laboratory Practice in Tropical Countries. In: Cremer, A. and Evan, G. (eds). Cambridge University Press, UK. Pp: 1-226.
[9]  Clinical and Laboratory Standards institute (CLSI) (2010). Methods for the dilution, antimicrobial susceptibility test for bacteria. Approved standards. Ninth Editions (M09).
[10]  Kabra, S.K., Lodha, R. and Pandey, R.M. (2010). "Antibiotics for community-acquired pneumonia in children". Cochrane Database System Reviews 3: Pp 1080-1090.
[11]  Obi, C.L., Ramalivhana, J., Momba, M.N.B., Onabolu, B., Igumbor, J.O., Lukoto, M., Mulaudzi, T.B., Bessong, P.O., Jasen van Rensburg, E.L., Green, E. and Ndou, S. (2007). Antibiotics resistance profiles and relatedness of enteric bacterial pathogens isolated from HIV/AIDS Patients with or without diarrhoea and their household drinking water in rural communities in Limpopo Province South Africa. Africa Journal of Biotechnology. 6 (8):1035-1047.
[12]  Anevlavis, S. and Bouros, D. (2010). "Community acquired bacterial pneumonia". Expert Opin Pharmacotherapy 11 (3): 361-74.
[13]  Gadkowski, L.B. and Stout, E. J. (2008). Cavitary Pulmonary Disease. Clinical Microbiology Reviews. 21, No. 2: 305-333.
[14]  Idris, M and Nasidi, A (2009). The Pathophysiology and clinical manifestations of HIV/AIDS. Aids in Nigeria. 56: 245-356.
[15]  Adeleye, A.I. (2010). Bacterial Bloodstream Infections in HIV-infected Adults Attending Lagos Teaching Hospital. Journal of Health, Population and Nutrition. 45 (13): 34-87.
[16]  Falcó, V., Fernández de Sevilla, T., Alegre, J., Barbé, J., Ferrer, A., Ocaña, I., Ribera, E., Martínez-Vázquez, J.M. (1994). Bacterial pneumonia in HIV-infected patients: a prospective study of 68 episodes. J. European Respiratory, 7, 235-239.
[17]  Franzetti, F., Cernuschi, M., Esposito, R., Moroni, M. (1992). Pseudomonas infection in patients with AIDS and AIDS related complex. Journal of Internal Medicine; 231: 437-443.
[18]  Franzetti, F., Grassini, A., Piazza, M., Degl’Innocenti, M., Bandera, A., Gazzola, L.,Marchetti, G. and Gor, A. (2007). Nosocomial Bacterial Pneumonia in HIV-Infected Patients: Risk Factors for Adverse Outcome and Implications for Rational Empiric Antibiotic Therapy Journal of Infection. Volume 34, Number 1, 9-16.
[19]  Okesola, A.O. and Oni, A.A. (2009). Antimicrobial resistance among common bacteria pathogens in South West, Nigeria. American Journal Agricultural environmental Science. 5(3): 327-330.
[20]  Wang, F., Zhu, D.M., Hu,P.P. and Zhang, Y.Y.(2001). Surveilance of bacterial resistance among isolates in Shanghai in 1999. Journal of infection chemotherapy. 7(2): 117-120.
[21]  El kholy, A.Basseem, H., Hall, G.S, Procop, G.W. and Lorngworth, D.L. (2003). Antimicrobial resistance in Cairo, Egypt, 1999-2000: a survey of five hospitals. Journal antimicrobial chemotherapy 51(3): 625-630.
[22]  Turner, D. and Dagan, R. (2001). The sensitivity of common bacteria to antibiotics in children in South Isreal, Harefuah. Amerian Journal of Epidemiology. 140(10): 923-929. eMedicine Dictionary, 2008. pneumonia. Pp. 2205-2230.
[23]  Oluyege, A.O., Dada, A.C., Ojo, A.M. and Oluwadare, E. (2009). Antibiotics resistance profile of bacterial isolates from food sold on University campuses in South West, Nigeria. Journal of Biotechnology. 8 (21) 5883-5887.
[24]  Ashby, B. and Turkington, C. (2007). The encyclopedia of infectious diseases (3 Ed.). New York: Facts on File. p. 242.
[25]  Jawertz, E, Melnick, J. and Adelberg, E.A. (2007). Hepatitis Viruses. In: Medical Microbiology. Brooks G.F, Butel J.S., Morse S.A. 22nd ed(s) Lange medical books/Mc Graw-Hill Medical publishing Division, New York. Pp 403-417.
[26]  Chang, C.C., Cheng, A.C. and Chang, A.B. (2007). "Over-the-counter (OTC) medications to reduce cough as an adjunct to antibiotics for acute pneumonia in children and adults". Cochrane Database System Rev (4): CD006088.
[27]  Isenberger, K. Hill, D.W., Jenkins, L.E. and Magee J.T. (2002). Clinical significance of the emergence of bacterial resistance in the hospital environment. Journal of Applied Microbiolog. 92: 908-978.
[28]  Lipps, G. (2008). Plasmid. Current Research and future trends. Caister Academic Press.
[29]  Kroll, J. Klinter, S., Schneider, C. and Steinbuchel, A. (2010). Plasmid addition systems: Perspective and application in biotechnology microbiology. Journal of Biotechnology. 3(6): 634-657.
[30]  Krause, D.C. and Balish, M.F. (2004). "Cellular engineering in a minimal microbe: structure and assembly of the terminal organelle of Mycoplasma pneumoniae". Molecular Microbiology 51 (4): 917-24.
[31]  Pratts, G., Mirelis, B. And Llovelt, T. (2000). Antibiotics resistance trends in enteropathogenic bacteria isolated in 1985-1987 and 1995-1998 in Barcelona. Antimicrobial agents chemotherapy. 44 : 1140-1145.