American Journal of Infectious Diseases and Microbiology
ISSN (Print): 2328-4056 ISSN (Online): 2328-4064 Website: http://www.sciepub.com/journal/ajidm Editor-in-chief: Maysaa El Sayed Zaki
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American Journal of Infectious Diseases and Microbiology. 2014, 2(1), 17-21
DOI: 10.12691/ajidm-2-1-4
Open AccessArticle

Evaluation of Microscopy, Culture and PCR Methods in the Laboratory Diagnosis of Genito-urinary Tuberculosis

Pranali Pingle1, , Pradeep Apte2 and Rakesh Trivedi3

1Microbiologist, Medicare Hospital and Research Centre, Indore

2Biochemist and Lab incharge, Medicare Hospital and Research Centre, Indore

3Principal, P.M.B.Gujarati Science College, Indore

Pub. Date: January 09, 2014

Cite this paper:
Pranali Pingle, Pradeep Apte and Rakesh Trivedi. Evaluation of Microscopy, Culture and PCR Methods in the Laboratory Diagnosis of Genito-urinary Tuberculosis. American Journal of Infectious Diseases and Microbiology. 2014; 2(1):17-21. doi: 10.12691/ajidm-2-1-4

Abstract

Genitourinary tuberculosis (GUTB) is the second most common form of extrapulmonary tuberculosis, with more than 90% of cases occurring in developing countries. In GUTB, the kidneys are the most common sites of infection and are infected through hematogenous spread of the bacilli, which then spread through the renal and genital tract. Diagnosis of TB is often delayed owing to the nonspecific nature of its presentation; therefore, a high degree of suspicion should be exercised and a systematic approach should be taken during investigation. The aim of this study was to apply bleach concentration method for detection of AFB in 5-day morning urine samples obtained from the suspects of urinary tuberculosis and to correlate the results with conventional Zeihl Neelsen (ZN) staining, TB culture and TB-PCR. A total of 46 samples were studied from clinically suspected cases of urinary tuberculosis. All the samples were processed for conventional ZN staining, Bleach concentration followed by ZN staining, TB culture on LJ media and TB-PCR (IS 6110) by standard protocols. Out of the 46 samples evaluated all were negative (0%) by conventional ZN staining, while the positivity increased to 7(15.22%) by bleach concentration method, the gold standard i.e. TB culture had 9(19.56%) positive and the TB-PCR gave 4(8.69%) positive. The results revealed that bleach concentration method was superior to conventional ZN staining method and TB-PCR. Though TB culture was found to be the best method, but it takes a long time for the diagnosis.

Keywords:
Genito-urinary tuberculosis M.tuberculosis conventional ZN staining bleach method PCR TB culture

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