American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2018, 6(1), 7-11
DOI: 10.12691/ajfst-6-1-2
Open AccessArticle

Acid, Bile and Aggregation Abilities of Lactobacillus plantarum Strains Isolated from Akamu a Nigerian Fermented Maize Food

Patience C. Obinna-Echem1, 2,

1School of Biological Sciences, Faculty of Science and Environment, Plymouth University, Plymouth, United Kingdom

2Food Science and Technology, Rivers State University, Nkpolu-Oroworukwo, Port Harcourt, Rivers State, Nigeria

Pub. Date: January 05, 2018

Cite this paper:
Patience C. Obinna-Echem. Acid, Bile and Aggregation Abilities of Lactobacillus plantarum Strains Isolated from Akamu a Nigerian Fermented Maize Food. American Journal of Food Science and Technology. 2018; 6(1):7-11. doi: 10.12691/ajfst-6-1-2


This study investigated the ability of two strains of Lactobacillus plantarum isolated from akamu a Nigerian fermented maize food to tolerate acid and bile condition. Auto-aggregation and co-aggregation with pathogens: Escherichia coli NCTC 11560 and Salmonella Enteritidis NCTC 5188 were also investigated. This was aimed at establishing preliminary probiotic potentials of these none intestinal L. plantarum isolates. Viability at pH 2 was significantly (p≤0.05) reduced from ≥8.26±0.05 to ≤4.94±0.49 Log10 CFU/mL after 3 h. Subsequent incubation in 0.3% ox gall bile media after 6 h enhanced growth to 5.73±0.13 and 7.93±0.12 Log10 CFU/mL for NGL5 and NGL7. The L. plantarum strains auto-aggregated but had no co-aggregation with the pathogens. After 5 h auto-aggregation at 37°C (>25%) was significantly (p≤0.05) greater than auto-aggregation at 22 - 24°C (<14%). The L. plantarum strains possessed abilities to survive passage through the GIT and auto-aggregated significantly at body temperature. This serves as a baseline data for further studies especially isolates that are not of intestinal origin.

Lactobacillus plantarum akamu acid and bile tolerance auto-aggregation co-aggregation

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