American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2017, 5(2), 53-60
DOI: 10.12691/ajfst-5-2-4
Open AccessArticle

The Effect of Steam Blanching and Drying Method on Nutrients, Phytochemicals and Antioxidant Activity of Moringa (Moringa oleifera L.) Leaves

Pierre Nobosse1, Edith N. Fombang1, and Carl M F. Mbofung1, 2

1Food Biophysics, Biochemistry and Nutrition Laboratory, Department of Food Science and Nutrition, National School of Agro-Industrial Sciences (ENSAI), University of Ngaoundere, P.O. Box 455, Ngaoundere, Adamawa Region, Cameroon

2College of Technology, University of Bamenda, P.O. Box 39, Bambili, North West Region, Cameroon

Pub. Date: February 20, 2017

Cite this paper:
Pierre Nobosse, Edith N. Fombang and Carl M F. Mbofung. The Effect of Steam Blanching and Drying Method on Nutrients, Phytochemicals and Antioxidant Activity of Moringa (Moringa oleifera L.) Leaves. American Journal of Food Science and Technology. 2017; 5(2):53-60. doi: 10.12691/ajfst-5-2-4


The antioxidant activity of plant materials is affected by post-harvest treatments. The present study was undertaken to evaluate the effects of steam blanching and two (solar and electric) drying methods on physicochemical composition, antioxidant activity (AOA) and rehydration properties of Moringa oleifera leaves. Fresh and blanched leaves of M. oleifera were dried by indirect solar-drying (≈35 ± 3°C, 12 h) and hot air electric drying (50°C, 5 h), and milled into flour (particle size ≤ 500 µm). Fresh, blanched and dried leaves were analyzed for their nutrient and phytochemical contents, antioxidant activity (Total Reducing Power (TRP) and 2,2-diphenyl-2-picryl hydrazyl (DPPH) scavenging activity) as well as rehydration properties (water absorption capacity (WAC) and water solubility index (WSI). Macronutrients content of M. oleifera leaves were unaffected by blanching and drying. Irrespective of drying method, drying had a significant negative effect (p < 0.05) on phytochemical contents, TRP and DPPH scavenging activity of M. oleifera leaves. Blanching prior to drying, however, dimmed the negative effect of the latter. Blanched leaves exhibited higher carotenoids content, TRP and WAC compared to unblanched leaves; whereas blanching caused a decrease in DPPH scavenging activity, vitamin C and WSI. This study highlights that fresh and blanched Moringa oleifera leaves are more suiTableas a source of dietary antioxidants than dry leaves.

Moringa oleifera leaves steam blanching drying antioxidant activity phytochemicals rehydration properties

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[1]  Rani, V., Yadav, Umesh. (2015). Free Radicals in Human Health and Disease. Springer India (Pvt.) Ltd., New Delhi.
[2]  Brieger, K., Schiavone, S., Miller, J.F.J., Krause, K.H. (2012). Reactive oxygen species: from health to disease. Swiss Medical Weekly, 142, w13659.
[3]  Satish, B.N., and Dilipkumar, P. (2015). Free radicals, natural antioxidants, and their reaction mechanisms. RSC Advances, 5, 27986-28006.
[4]  Brewer, M.S. (2011). Natural antioxidants: Sources, compounds, mechanisms of action, and potential applications. Comprehensive Reviews in Food Science and Food Safety, 10, 221-247.
[5]  Taghvaei, M., and Jafari, S.M. (2015). Application and stability of natural antioxidants in edible oils in order to substitute synthetic additives. Journal of Food Science and Technology, 52(3), 1272-1282.
[6]  Rodríguez-Pérez, C., Quirantes-Piné, R., Fernández-Gutiérrez, A., Segura-Carretero, A. (2015). Optimization of extraction method to obtain a phenolic compounds-rich extract from Moringa oleifera Lam leaves. Industrial Crops and Products, 66, 246-254.
[7]  Agamou, A.J.A., Fombang, E.N., and Mbofung, C.M.F. (2015). Particular benefits can be attributed to Moringa oleifera Lam leaves based on origin and stage of maturity. Journal of Experimental Biology and Agricultural Sciences, 3(6), 541-555.
[8]  Saini, R.K., Shetty, N.P., and Giridhar, P. (2014). Carotenoid content in vegetative and reproductive parts of commercially grown Moringa oleifera Lam. cultivars from India by LC–APCI–MS. European Food Research Technology, 238, 971-978.
[9]  Mutiara, T.K., Harijono, T.E., Endang, S. (2012). Nutrient content of Kelor (Moringa oleifera Lam.) leaves powder under different blanching methods. Food and Public Health, 2, 296-300.
[10]  Joshi, P., Mehta, D. (2010). Effect of dehydration on the nutritive value of drumstick leaves. Journal of Metabolomics and Systems Biology, 1, 1-5.
[11]  Gupta, S., Lakshmi, J., Prakash, J. (2008). Effect of different blanching treatments on ascorbic acid retention in green leafy vegetables. Natural product radiance, 7, 111-116.
[12]  Nambiar, V.S, Matela, H.M., Baptist, A. (2013). Total antioxidant capacity using ferric reducing antioxidant power and 2, 2‑diphenyl‑1 picryl hydrazyl methods and phenolic composition of fresh and dried drumstick (Moringa oleifera) leaves. International Journal of Green Pharmacy, 7, 66-72.
[13]  Potisate, Y., Phoungchandang, S. and Kerr, W.L. (2014). The effects of predrying treatments and different drying methods on phytochemical compound retention and drying characteristics of Moringa leaves (Moringa oleifera Lam.). Drying Technology, 32, 1970-1985.
[14]  Saini, R.K., Shetty, N.P., Prakash, M., Giridhar, P. (2014). Effect of dehydration methods on retention of carotenoids, tocopherols, ascorbic acid and antioxidant activity in Moringa oleifera leaves and preparation of a RTE product. Journal of Food Science and Technology, 51(9), 2176-2182.
[15]  Ndiaye, C., Xu, S.Y., Wang, Z. (2009). Steam blanching effect on polyphenoloxidase, peroxidase and colour of mango (Mangifera indicaL.) slices. Food Chemistry, 113, 92-95.
[16]  AOAC (1990). Official Methods of Analysis (15th ed, Vol 2) AOAC Inc, Washington DC.
[17]  AOAC (1974). Carotenes and Xanthophylls in Dried Plant Materials and Mixed Feeds: Spectrophotometric Method. AOAC Official Methods, 970.64-1974, 2 pages.
[18]  Rodriguez-Amaya, D.B. (2001). A guide to carotenoids analysis in foods. ILSI PRESS, Washington DC.
[19]  Makkar Harinder, P.S., Siddhuraju, P., Becker, K. (2007). Plant Secondary Metabolites. Humana Press, Totowa, New Jersey.
[20]  Adom, K., Sorrells, M., Liu, R. (2003). Phytochemical profiles and antioxidant activity of wheat varieties. Journal of Agricultural and Food Chemistry, 51, 7825-7834.
[21]  Yen, G.C., Chen, H.Y. (1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and Food Chemistry, 43, 27-32.
[22]  Brand-Williams, W., Cuvelier, M.E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebensm-Wiss Technol, 28, 25-30.
[23]  Phillips, R.D., Chinnan, M.S., Branch, A.L., Miller, J., Mcwatters, K.H. (1988). Effects of Pretreatment on Functional and Nutritional Properties of Cowpea Meal. Journal of food science, 53, 805-809.
[24]  Anderson, R.A., Conway, H.F., Pfeifer, V.F., Griffin, E.L. (1969). Gelatinization of corn grits by roll- and extrusion-cooking. Cereal Science Today, 14, 4-12.
[25]  Amaglo N.K. et al (2010). Profiling selected phytochemicals and nutrients in different tissues of the multipurpose tree Moringa oleifera L. grown in Ghana. Food Chemistry, 122, 1047-1054.
[26]  Sánchez-Machado, D.I., Núñez-Gastélum, J.A., Reyes-Moreno, C., Ramírez-Wong, B., López-Cervantes, J. (2010). Nutritional quality of edible parts of Moringa oleifera. Food Analysis Methods 3, 175-180.
[27]  Ferracane, R., Pellegrini, N., Visconti, A., Graziani, G., Chiavaro, E., Miglio, C., Fogliano, V. (2008). Effects of different cooking methods on antioxidant profile, antioxidant capacity, and physical characteristics of Artichoke. Journal of Agricultural and Food Chemistry, 56, 8601-8608.
[28]  Oyetade, O.A., Oyeleke, G.O., Adegoke, B.M., Akintunde, A.O. (2012). Stability studies on ascorbic acid (vitamin c) from different sources. IOSR Journal of Applied Chemistry, 2, 20-24.
[29]  Gupta, S., Gowri, B.S., Lakshmi, A.J., Prakash, J. (2013). Retention of nutrients in green leafy vegetables on dehydration. Journal of Food Science and Techology Mysore, 50, 918-925.
[30]  Adefegha, S.A., Oboh, G. (2011). Enhancement of total phenolics and antioxidant properties of some tropical green leafy vegetables by steam cooking. Journal of Food Processing and Preservation, 35, 615-622.
[31]  Djuikwo, N.V., Ejoh, A.R., Gouado, I., Mbofung, C.M.F., Tanumihardjo, S.A. (2011). Determination of major carotenoids in processed tropical leafy vegetables indigenous to Africa. Food and Nutrition Sciences, 2, 793-802.
[32]  Palermo, M., Pellegrini, N., Fogliano, V. (2014). The effect of cooking on phytochemical content in vegetables: A Review. Journal of the Science of Food and Agriculture, 94, 1057-1070.
[33]  Pellegrini, N., Chiavaro, E., Gardana, C., Mazzeo, T., Contino, D., Gallo, M., Patrizi, A., Fogliano, V., Porrini, M. (2010). Effect of different cooking methods on color, phytochemical concentration, and antioxidant capacity of raw and frozen Brassica vegetables. Journal of Agricultural and Food Chemistry, 58, 4310-4321.
[34]  Blumberg, J.B., Vita, J.A., Chen, C.Y. (2015). Concord grape juice polyphenols and cardiovascular risk factors: dose-response relationships. Nutrients, 7, 10032-10052.
[35]  Yakubu, N., Amuzat, A.O., Hamza, R.U. (2012). Effect of processing methods on the nutritional contents of bitter leaf (Vernonia amygdalina). American Journal of Food and Nutrition, 2, 26-30.
[36]  Wangcharoen, W., Gomolmanee, S. (2013). Antioxidant activity changes during hot-air drying of Moringa. Maejo International Journal of Science and Technology, 7, 353-363.
[37]  Salvamani, S., Gunasekaran, B., Shaharuddin, N.A., Ahmad, S.A., Shukor, M.Y. (2014). Antiartherosclerotic effects of plant flavonoids. BioMed Research International.
[38]  Anderson, J.W. et al (2009). Health benefits of dietary fiber. Nutrition Reviews, 67, 188-205.
[39]  Mbofung, C.M.F., Njintang, Y.N., Waldron K.W. (2002). Functional properties of cowpea–soy–dry red beans composite flour paste and sensorial characteristics of akara (deep fat fried food): effect of whipping conditions, pH, temperature and salt concentration. Journal of Food Engineering, 54, 207-214.
[40]  Djantou, N.E.B. (2006). Optimisation du broyage des mangues séchées (Manguifera indica var Kent): Influence sur les propriétés physicochimiques et fonctionnelles des poudres obtenues. Thesis, INPL, Nancy, France.