American Journal of Food Science and Technology
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American Journal of Food Science and Technology. 2016, 4(4), 102-108
DOI: 10.12691/ajfst-4-4-3
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Proximate, Anti-Nutritional and Phytochemical Composition of the Yellow Variety of the Synsepalum Dulcificum (Miracle Fruit) Berry

Njoku N. E.1, , Ubbaonu C. N.1, Alagbaoso S.O.1, Agunwa I.M1 and Eluchie C.N.1

1Department of Food Science and Technology, Federal University of Technology, Owerri, ImoState, Nigeria

Pub. Date: May 30, 2016

Cite this paper:
Njoku N. E., Ubbaonu C. N., Alagbaoso S.O., Agunwa I.M and Eluchie C.N.. Proximate, Anti-Nutritional and Phytochemical Composition of the Yellow Variety of the Synsepalum Dulcificum (Miracle Fruit) Berry. American Journal of Food Science and Technology. 2016; 4(4):102-108. doi: 10.12691/ajfst-4-4-3


The pulp of freshly harvested mature Synsepalumdulcificum (miracle fruit) berries were analyzed for proximate, pH, titratable acidity, anti-nutritional and phytochemical content. The results indicated a moisture content of 45.12%, protein content of 2.48% and a carbohydrate content of 48.84%.The anti-nutrients content include Tannin 2.90 ± 0.64mg/100g, phytate 5.21 ± 0.92 mg/100g, glycosidic cyanide 0.03 ± 0.00mg/100g, steroid 1.56±0.03 mg/100g and oxalate 11.04 ± 0.29%. The anti-nutritional levels were all within the recommended safety limits. Some phytochemicals like flavonoids, resins and steroids were also identified. It is hoped that the knowledge of miracle fruit’s nutritional content/importance will enhance its utilization in our diet.

berry pulp phytochemicals proximate anti-nutrients Synsepalumdulcificum

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[1]  Njoku, N.E.,Ubbaonu, C.N.Alagbaoso, S.O., Eluchie, C.N. and Umelo, M.C. (2015). Amino acid profile and oxidizable vitamin content of Synsepalumdulcificum berry (miracle fruit) pulp.Food Science &Nutrition. 3(3): 252-256.
[2]  Opeke, I. K. (1984). Growth and morphogenesis of mature embryo of Capsella in culture. Plant Physiology 39: 691-699.
[3]  Njoku, N.E. (2014). Some phytochemical components and anti-nutritional composition of the yellow variety of Synsepalumdulcificum berry (miracle fruit). Proceedings of the 38th Annual Conference and General Meeting of NIFST, Lagos. pp 147-148.
[4]  Wilson, I. and Wilson D., (2006). Its a Miracle. Rare Fruit Review [online]. Available at: [Accessed January 20, 2012].
[5]  AOAC. (1994). Association of Official Analytical Chemists. Official Methods of Analysis. Washington, D.C.
[6]  Harbourne, J. B. (1973). Phytochemical Methods. A guide to Modern Technology ofPlant Analysis, 2nd ed. Chapman and Hall, New York. Pp: 88-185.
[7]  Trease, G. E. and Evans, W. C. (1996). Phenols and Phenolic Glycosides in Trease and Evans Pharmacology and Bikere. Tindall, London. Pp. 832-836.
[8]  Pearson, D. A. (1976). Chemical Analysis of Foods. 7th Edition. Church Hill Livingstone, New York.1:422-511.
[9]  Amtfield, S.D., Ismond, M.A.H. and Murray, E.D. (1985). The fate of anti-nutritional factors during the preparation of faba bean protein isolates using a micellization technique. Canadian Institute of Fd. Sci. Tech. J. 18 (2):137-143.
[10]  Onwuka, G. I. (2005). Food Analysis and Instrumentation: Theory and Practice. Naphtali prints, Lagos. Pp 156-161.
[11]  Chen, S. C. (1992).Physicochemical principles for the concentration and freezing of fruit juices. In: Fruit Processing Technology. AgScience, Inc: Florida Pp. 23-25.
[12]  Konja, G. and Lovric, T. (1993). Berry Fruit Juice Processing Technology. Agscience, Inc: Florida
[13]  Edem, C. A. and Dosunmu, M. I. (2011). Chemical evaluation of proximate composition, ascorbic acid and anti-nutrients content of African star apple (Chrysophyllumafrcanum) fruit. IJRRAS 9(1):146-149.
[14]  Belitz, H.D., Grosch, W. and Schieberle, P. (2009). Springer fruits. Food Chemistry. Available at: [Accessed January 20, 2012].
[15]  Ihekoronye, A. I. and Ngoddy, P. O. (1985). Integrated Food Science and Technologyfor the Tropics. Macmillan Publishers Ltd, London. Pp. 65-193, 283-294.
[16]  Nwofia, G. E., Ojimelukwe, P. and Eji, C. (2012). Chemical composition of leaves, fruit pulp and seeds in some Caricapapya (L) morphotypes. International Journal ofMedicinal and Aromatic Plants, 2 (1): 200-206.
[17]  Eromosele, I.C. and Eromosele, C.O. (1993). Studies on the chemical composition and physio-chemical properties of seeds of some wild plants. Plant Food Hum. Nutr. 43: 251-258.
[18]  ANON, (1960). Food Composition Tables. Rome: FAO. 1:16
[19]  Gonzalez, M., Hernandez, Y. and Lobo, M. G. (2012). Determination of Vitamin C in tropical fruit: a comparative evaluation of methods. Plant Physiology Laboratory, Department of Tropical Fruits, Spain: Instituto Canario de Investigaciones Agrarias, Apdo. Available at [Accessed January 20, 2012].
[20]  Premier, R. (2002). Phytochemical composition: A paradigm shift for food-health considerations. Asia Pacific J ClinNutr. 11(6): 197-201.
[21]  Dillard, C.J. and German, J.B. (2000).Phytochemicals: Nutraceuticals and human health. J Sci Food Agric., 80: 1744-1756.
[22]  Seeram, N.P. (2006). Berries. In: D. Heber et al. (eds). Nutritional Oncology, 2nd ed. Academic Press, London, UK. p. 615-625
[23]  Seeram, N.P. and Heber, D. (2006). Impact of berry phytochemicals on human health: Effects beyond antioxidation. In: C.T. Ho et al. (eds). Lipid Oxidation and Antioxidants: Chemistry, Methodologies and Health Effects. ACS Symposium Series. Oxford University Press.
[24]  Manach, C. and J.L. Donovan. 2004. Pharmacokinetics and metabolism of dietary flavanoids in humans. Free Radical Research, 38:771-785.
[25]  Cody, V., Middleton, V.E. and Harbourne, J.B. (1986). Plant flavonoids in biology and medicine: Biochemical, pharmacological and structure–activity relationships. New York: Alan Liss,
[26]  Gabor, M. (1986). Anti-inflammatory and anti-allergic properties of flavonoids. In: Cody, V., Middleton, V.E., Harbourne, J.B., eds. Plant flavonoids in biology and medicine: Biochemical, pharmacological and structure–activity relationships. New York: Alan Liss.
[27]  Tiwar, P., Kumar, B., Kaur, M., Kaur,G. and Kaur, H. (2011).Phytochemical screening and Extraction: A ReviewInternationale Pharmaceutica Sciencia, Vol 1, issue 1
[28]  Stray, F. (1998). The national guide to medicinal herbs and plants. Tiger books International: London. Pp 12-16.
[29]  Morton, J. (2001). Purple mombin fruits of warm climaes. Miami Publishers: New York, p.245
[30]  Kozioc, M.J. and Marcia, M.J. (2004). Chemical composition nutritional evaluation and economic prospects of Spondias purpurea (Anaracardiaceae). Econ Bot., 52: 373-380
[31]  Bello, M. O., Falade, O. S., Adewusi, S. R. andOlawore, N. O.(2008). Studies on the chemical compositions and anti-nutrients of some lesser known Nigeria fruits. African J. Biotechn.7 (21): 3972-3979
[32]  Rathod, V. S. and Valvi, S. R. (2011). Antinutritional factors of some wild edible fruits from Kolhapur District. Recent Research in Science and Technology 3(5): 68-72.
[33]  Inuwa, H. M., Aina, V. O., Gabi, B., Aimola, I. and Amao, T. (2011). Comparative determination of antinutritional factors in groundnut oil and palm oil. Advance Journal of Food Science and Technology, 3(4): 275-279.
[34]  Tapiero, H., Tew, K. D., Nguyen, B. G. and Mathe, G. (2002). Polyphenols: do they play a role in the prevention of human pathologies? Biomed. Pharmacother 56: 200-207.
[35]  Suree, N., Surat, K. and Akekachai, N. (2004). Phytate and Fiber Content in Thai Fruits commonly consumed by diabetic patients. J. Med. Assoc. Thai 87(12): 1444-1446.
[36]  Thompson, L. U. (1993). Potential health benefits and problems associated with anti-nutrients in foods. Food Res. International. 26:131-149.
[37]  Gernah, D. I., Atolagbe, M. O. and Echegwo, C. C. (2007). Nutritional composition of the African locust bean (Parkiabiglobosa) fruit pulp. Nutritional Composition of African locust Bean. Nigerian Food Journal, 25 (1):190-196. Available at: [Accessed January 20, 2012].
[38]  Awe, I. S. and Sodipo, O. A. (2001). Nigerian Journal of Food Science and Nutrition. 3: 103-107.
[39]  Tannenbaum, S. R. (1979) Nutritional and Safety Aspects of Food Processing. New York: Marcel Dekker Inc. Pp. 265-295.
[40]  Umaru, H. A., Adamu, R., Dahiru, D. and Nadro, M. S. (2007). Levels of antinutritional factors in some wild edible fruits of Northern Nigeria. African J.Biotech.6(16):1935-1938.
[41]  Adewusi, S. R. A. and Osuntogun, B. A. (1991). Effects of cooking on tannin content, trypsin inhibitor activity and in-vitro digestibility of some legume seeds in Nigeria. Nigeria Food Journal 9: 139-145.
[42]  Aletor, V. A. and Fetuga, B. L. (1987). Pancreatic and intestinal amylase (EC in the rat fed haemagglutininextract.II Evidence of impaired dietary starch utilization. J. Anim. Physiol. Anim. Nutr. 57(3):113-117.
[43]  Onyeka, E. U and Nwambekwe, I. O. (2007). Phytochemical profile of some greenleafy vegetables in South-East Nigeria. Nigerian Food Journal 25 (1): 67-72. Available at: [Accessed May 21, 2012].
[44]  Piironen, V., Toivo, J., and Lampi, A. M. (2002). New data for cholesterol contents in meat, fish, milk, eggs and their products consumed in Finland. Journal of FoodComposition and Analysis 15: 705-713.