American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2016, 4(1), 1-6
DOI: 10.12691/ajfst-4-1-1
Open AccessArticle

Efect of Phytol on Dehydrogenase Activity of Bacterial Isolates from Grilled Meat


1Department of food Science and Technology, Federal University of Technology P.M.B 1526, Owerri Imo State

2Department of Industrial Microbiology, Federal University of Technology P.M.B 1526, Owerri Imo State

Pub. Date: January 04, 2016

Cite this paper:
ELUCHIE C.N., ORANUSI S., AKUJOBI C. O. and ALAGBAOSO S.O.. Efect of Phytol on Dehydrogenase Activity of Bacterial Isolates from Grilled Meat. American Journal of Food Science and Technology. 2016; 4(1):1-6. doi: 10.12691/ajfst-4-1-1


Effect of phytol on the dehydrogenase activity of Staphylococcus aureus Bacillus cereus, Pseudomonas aeruginosa and Enterococcus faecalis isolated from grilled meat was examined via dehydrogenase assay using 2,3,5 – tripheny tetrazolium chloride (TTC) as the electron acceptor. The bacteria isolates were exposed to various concentrations of phytol (0-2000 μg) in a nutrient broth. The minimum inhibitory concentration (MIC) and minium bactericidal concentration (MBC) values of phytol against the bacterial isolates were found in the range of 31.25 μg /ml to 250 μg /ml and 125 μg /ml to 250 μg /ml respectively. Effect of phytol on the dehydrogenase activity of the bacterial isolates showed that gram negative Pseudomonas aeruginosa had higher dehydrogenase activity than the gram positive Bacillus cereus, Enterococcus faecalis and Stpahylococcus aureus. The effect of phytol on the dehydrogenase activity varied significantly among the bacterial isolates. This result indicates that phytol could be used as an antibacterial agent in food industry to inhibit the growth of certain food borne pathogens.

Phytol dehydrogenase activity grilled meat

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[1]  Aiyelaagbe, O.O., Adesoganm, k., Ekundayo, O., and Gloer, J.B. (2007). Antibacterial Diterpenoids from Jatropha podagrica Hook; Phytochemistry 68: 2420-2425.
[2]  Alisi, C.S., Nwanyanwu C.E, Akujobi, C.O and Ibegbulem, C.O. (2008). Inhibition of Dehydrogenase Activity in Pathogenic Bacteria Isolates by Aqueous Extracts Musa paradisiaca var sapientum. African Journal of Biotechnology 7(12): 1821-1825.
[3]  Arai, T., Hamashima, H., and Sasatsu, M. (1996). Inhibiting Effects of Fatty Acids, Purified Camellia Oil and Olive Oil on the Growth of Staphylococcus aureus. Jpn. J. Chemother. 44: 786-791.
[4]  Bajpai, V.K., Al-Reza, S.M., Lee, J.H., and kang, S.C. (2009a). Chemical Composition, Antibacterial and Anxioxidant Activities of Leaf Essential Oil and Extracts of Motasequoia glytostroboides. Miki ex Hu; Food Chem. Toxicol. 47: 1876-1883.
[5]  Daines, A.M. (2003). The Synthesis of Naturally Occurring Vitamin K and Vitamin K Analogues. Current Org. Chem. 7: 1625-1634.
[6]  Dellar, J.E., Cole, M.D and Waterman, P.G. (1996). Antimicrobial Abietane Diterpenoids from Plectranthus elegan. Phytochemistry 41.735-738.
[7]  Netscher, T. (2007). Synthesis of Vitamin E. Vit. and Hormones. 76: 155- 2002.
[8]  Nweke, C.O., Alisi, C.S., Okolo, J.C. and Nwanyanwu, C.E. (2007). Toxicity of Zinc to Heterotrophic Bacteria of Tropical River Sediments. Appl. Eco. Envron. Res. 5(1): 123-132.
[9]  Nweke, C.O., Okolo, J.C., Nwanyanwu, C.E and Alisi, C.S. (2006). Response of Planktonic bacteria of New Calabar River to Zinc Stress. Afr. J. Biotechnol. 5(8): 653-658.
[10]  Nwogu, L.A., Alisi, C.S, Ibegbulem, C.O, Igwe, C.U. (2007). Phytochemical and antimicrobial activity of ethanolic extract of Landolphia owariensis leaf. Afr. J. Biotechnol. 6(7):890-893.
[11]  Nwogu, L.A., Alisi, C.S, Igwe, C.U., Ujowundu, C.O. (2008). A Comparative Study of the antimicrobial properties of the ethanolic extracts of Landolphia owariensis leaf and root. Afri. J. Biotechnol. 7(4): 368-372.
[12]  Onuh, M.O. and Igwemma, A.A. (2000). Applied Statistical Techniques for Business and Basic Sciences. Skillmark Media Ltd. Owerri, Imo State. Pp. 176-190, 205-215, 322-338.
[13]  Praveen-Kumar, J.C. (2003). 2,3,5 – Triphenytetrazolium Chloride (TTC) and Electron Acceptor of Culturable Cell Bacteria, Fungi and Antinomycetes. Biol. Fert. Soil 38:186-189.
[14]  Rotani, J.F and Volkman, J.K (2003). Organic Geochemistry. Phytol Degradation Products as Biogeochemical Tracers in Aquatic Environments. 34, 1-35.
[15]  Tauxe, V.R. (2002), Emerging Food Borne Pathogens. Int. J. Food Microbial. 78: 31-34.
[16]  Ulubelen, A., Oksuz, S., Kolak, U., Bozok-Johansson, C., Celik, C., and Voelter, W. (2000). Antibacterial diterpenes from the Roots of Salvia viridis; Planta Med.66: 458- 462.