Biochemical Analysis of Chickpea Accessions vis-a-vis; Zinc, Iron, Total Protein, Proline and Antioxidant Activity

Sameer S. Bhagyawant¹, Neha Gupta¹ and Nidhi Shrivastava^2,

¹School of Studies in Biotechnology, Jiwaji University, Gwalior, India

²Department of Bioscience & Biotechnology, Banasthali University, India

Cite this paper:
Sameer S. Bhagyawant, Neha Gupta and Nidhi Shrivastava. Biochemical Analysis of Chickpea Accessions vis-a-vis; Zinc, Iron, Total Protein, Proline and Antioxidant Activity. American Journal of Food Science and Technology. 2015; 3(6):158-162. doi: 10.12691/ajfst-3-6-3

Abstract

Legumes are good source of proteins and micronutrients. Chickpea is one such preferred legume source of protein after milk only. Development of chickpea cultivars with high concentration of zinc (Zn) and iron (Fe) is one of the prime objective of breeding programmes. The aim of this study was to analyze chickpea seeds and compare their biochemical properties. Biochemical characterization of 20 different chickpea seed accessions vis-a-vis total protein, proline and antioxidant activity was carried out. Atomic absorption spectroscopy was employed to quantify zinc and iron contents. Significant variation in biochemical composition of chickpea was noted for total protein (144.59 mg/g), proline (15.89 mg/100g) and antioxidant activity (48.2%) in all the accessions that are tested. The average content of Zn and Fe across these accessions was observed in a range of 0.37 and 0.91 mg respectively. ILWC-257 contained highest Zn (0.66 mg) and Fe (2.76 mg). The overall results indicated that the chickpea seeds exhibit different biochemical properties.

Keywords:
chickpea Fe and Zn proline

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References:

[1]	Brown, K. H., Peerson, J. M., Rivera, J. & Allen, L., Effect of supplemental zinc on the growth and serum zinc concentrations of prepubertal children: a meta-analysis of randomized controlled trials, American Journal of Clinical Nutrition, 75(6), 1062-71, 2002.
[2]	Khatoon, N. & Prakash, J., Nutritional quality of microwave-cooked and pressure-cooked legumes, Int J Food Sci Nutrition, 55(6), 441-448, 2004.
[3]	Jukanti, A. K., Gaur, P. M., Gowda, C. L. & Chibbar, R. N., Nutritional quality and health benefits of chickpea (Cicer arietinum L.): a review, Br J Nutrition, 108, 11-26, 2012.
[4]	Marin, J.A., Andreu, P., Carrasco, A. & Arbeloa, A., Determination of proline concentration, an abiotic stress marker, in root exudates of excised root cultures of fruit tree rootstocks under salt stress, Revue des RégionsArides – Numéro special, 24, 722-727, 2010.
[5]	Oury, F. X., Leenhardt, F., Balfourier, F. & Charmet, G., Genetic variability and stability of grain magnesium, zinc and iron concentrations in bread wheat, Eur. J. Agronomy, 25, 177-185, 2006.
[6]	Umran, H., Canan, O., Sermin, C., Ali, O. & Serap, F. E., Major-minor element analysis in some plant seeds consumed as feed in Turkey, Natural Science, 4(5), 298-303, 2012.
[7]	Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., Protein measurement with the Phenol Reagent, J. Biol. Chemistry, 193, 265-275. 1951
[8]	Bates, L., Waldren, R. P. & Teare, I. D., Rapid determination of free proline for water-stress studies, Plant and Soil, 39, 205-207, 1973.
[9]	Awah, F. M., Uzoegwu, P. N., Oyugi, J. O., Rutherford, J., Ifeonu, P. & Yao, X., Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract, Food Chemistry, 119, 1409-1416, 2010.
[10]	Gyamfi, M. A., Yonamine, M., & Aniya, Y., Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries, General Pharmacology, 32, 661-667, 1999.
[11]	Herber, R.F.M. & Stoeppler, M.: Eds. Trace Element Analysis in Biological Specimens, Elsevier, New York 1994.
[12]	Kaur, K., Kaur, N., Gupta, A. K., and Singh I., Exploration of the antioxidative defense system to characterize chickpea genotypes showing differential response towards water deficit conditions, Plant Growth Regul, 70; 49-60, 2013.
[13]	Khedr, A. H., Abbas, M. A., Wahid, A. A., Quick, W. P. & Abogadallah, G. M., Proline induces the expression of salt-stress-responsive proteins and may improve the adaptation of Pancratium maritimum L. to salt-stress, J Exp Bot., 54, 2553-2562, 2003.
[14]	Raymond, M. J. & Smirnoff, N., Proline Metabolism and Transport in Maize Seedlings at Low Water Potential, Ann Bot, 89, 813-823, 2002.
[15]	Marshner, H., Mineral Nutrition of Higher Plants, 2nd edn. Academic Press, London, 889 1995.
[16]	Peleg, Z., Saranga, Y., Yazici, A., Fahima, T., Ozturk, L. & Cakmak, I., Grain zinc, iron and protein concentrations and zinc-efficiency in wild emmer wheat under contrasting irrigation regimes, Plant Soil, 306, 57-67, 2008.
[17]	Kaur, N., Kumar, A., Kaur, K., Kaur, S., Gupta, A. K. & Singh, I., Antioxidant Enzymes and DPPH-Radical Scavenging Activity in Chilled and Heat-Shocked Rice (Oryza sativa L.) Seedlings Radicles, Proc. Natl. Acad. Sci, 85(2), 615-623, 2015.
[18]	Asher, C.J. Beneficial elements, functional elements and possible new essential elements, In: Micronutrient in Agriculture 2nd Ed. Soil Science Society of America, Inc. Madison, WI, USA 703-723, 1991.
[19]	Bressani, R. & Elias, L.G., Nutritional value of legume crops for human and animals. Advances in legume Science (Summerfield, R.J. and Bunting, AH Eds.) Royal Botanic Gardens, Kew. P. 135, 1980.
[20]	Louis, G., Stéphane, M. & Wolfgang, S., Iron in seeds – loading pathways and subcellular localization, Frontiers in Plant Science Plant Nutrition, 4, 535, 2014.
[21]	Kumar, O. A., & Tata, S. S., SDS-PAGE Seed Storage Protein Profiles in Chili Peppers (Capsicum L.), Notulae Scientia Biologicae, 2(3), 86-90, 2010.
[22]	El-Adawy, T. A., Nutritional composition and antinutritional factors of chickpeas (Cicer arietinum L.) undergoing different cooking methods and germination, Plant Foods for Human Nutr, 57(1), 83-97, 2002.