American Journal of Food Science and Technology
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American Journal of Food Science and Technology. 2015, 3(5), 118-125
DOI: 10.12691/ajfst-3-5-1
Open AccessArticle

Inhibitory Activity of Lactobacillus plantarum Strains from Akamu - A Nigerian Fermented Maize Food against Escherichia coli

Patience C. Obinna-Echem1, 2,

1School of Biological Sciences, Faculty of Science and Environment, Plymouth University, Plymouth, United Kingdom

2Food Science and Technology, Rivers State University of Science and Technology, Port Harcourt, Rivers State, Nigeria

Pub. Date: September 28, 2015

Cite this paper:
Patience C. Obinna-Echem. Inhibitory Activity of Lactobacillus plantarum Strains from Akamu - A Nigerian Fermented Maize Food against Escherichia coli. American Journal of Food Science and Technology. 2015; 3(5):118-125. doi: 10.12691/ajfst-3-5-1


Lactobacillus plantarum strains: NGL5 and NGL7 previously identified from akamu-a lactic acid bacteria fermented maize food using PCR sequencing analysis were evaluated for inhibitory activity against Eschericia coli NCTC 11560 in fermenting ground maize slurries at 22 and 30°C, and in porridges prepared from 24 h fermented slurries. The L. plantarum strains fermented ground maize slurries and produced significant levels of titratable acidity ≥5 g L-1 and low pH ≤3.63, displaying inhibitory activity against E. coli NCTC 11560. Inhibition was significantly (p≤0.05) greater at 30°C than at 22°C. At 30°C, E. coli was inhibited after 24 h in the co-inoculation assay (µ < -0.17 h-1; D value <10 h) and after 180 min in the already fermented slurries (µ < -0.13 min-1; D value <14 min). In the porridges, E. coli decreased below detection limit after 20 mins. This study showed that E. coli NCTC 11560 introduced during or after fermentation and into freshly prepared porridges from the L. plantarum strains fermented slurries would be unable to survive and proliferate at pH <4.2 and acidity >5 g L-1. For safety of the product however fermentation has to be for at least 24 h at 30°C or more at 22°C.

Lactobacillus plantarum maize slurry fermentation acidity inhibition Escherichia coli

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[1]  Adams, M.R. and L. Nicolaides, Review of the sensitivity of different foodborne pathogens to fermentation. Food Control, 8(5-6): 227-239. 1997.
[2]  Akingbala, J.O., L.W. Rooney, and J.M. Faubion, A laboratory procedure for the preparation of Ogi, a Nigerian fermented food. Journal of Food Science, 46(5): 1523-1526. 1981.
[3]  Teniola, O.D. and S.A. Odunfa, The effects of processing methods on the levels of lysine, methionine and the general acceptability of Ogi processed using starter cultures. International Journal of Food Microbiology, 63(1-2): 1-9. 2001.
[4]  Inyang, C.U. and C.A. Idoko, Assessment of the quality of ogi made from malted millet. African Journal of Biotechnology 5 (22): 2334-2337. 2006.
[5]  Obinna-Echem, P.C., V. Kuri, and J. Beal, Evaluation of the microbial community, acidity and proximate composition of Akamu, a fermented maize food. Journal of the Science of Food and Agriculture,. 94(2): 331-340. 2014.
[6]  Afifi, Z.E.M., et al., Contamination of weaning foods: Organisms, channels, and sequelae. Journal of Tropical Pediatrics., 44: 335-337. 1998.
[7]  Lawal, A.K., O.B. Oyedoyin, and O.O. Olatunji, Fate of pathogenic bacteria during fermentation of cereal porridge ("Ogi")-a weaning food formula. Nigerian Food Journal, 27(1): 19-26. 2009.
[8]  Motarjemi, Y., et al., Contaminated weaning food: a major risk factor for diarrhoea and associated malnutrition. Bulletin of the World Health Organization, 71(1): 79-92. 1993.
[9]  Nyatoti, V.N., S.S. Mtero, and G. Rukure, Pathogenic Escherichia coli in traditional African weaning foods. Food Control, 8(1): 51-54. 1997.
[10]  Bakare, S., et al., Comparison of the survival of diarrhoeagenic agents in two local weaning foods (Ogi and Koko). Journal of Tropical Paediatrics, 1998. 44 p. 332-334.
[11]  Mensah, P.P., et al., Fermentation of cereals for reduction of bacterial contamination of weaning foods in Ghana. The Lancet, 336(8708): 140-143.1990.
[12]  Odugbemi, T., et al., Bacteriological study of cooked Ogi (fermented cereal weaning food) and its potential safety in a rural Nigerian community. Transactions of the Royal Society of Tropical Medicine and Hygiene, 87(2): 234-235. 1991.
[13]  Ohenhen, R.E. and M.J. Ikenebomeh, Shelf Stability and Enzyme Activity Studies of Ogi: A corn meal fermented product. Journal of American Science, 3(1): 38-42. 2007.
[14]  Osundahunsi, O.F. and O.C. Aworh, Nutritional evaluation, with emphasis on the protein quality of maize-based complementary foods enriched with soya bean and cowpea Tempe. International Journal of Food Science and Technology, 38: 809-813. 2003.
[15]  Otunola, E.T., O. Ogunsola, and V.F. Abioye, Effect of the addition of Tempeh on some properties of 'Agidi', a West Africian fermented maize gel. International Journal of Food and Agricultural Research, 3(1): 119-128. 2006.
[16]  Teniola, O.D. and S.A. Odunfa, Microbial assessment and quality evaluation of Ogi during spoilage. World Journal of Microbiology and Biotechnology, 18(8): 731-737. 2002.
[17]  Miles, A.A. and S.S. Misra, The estimation of the bactericidal power of the blood. Journal of Hygiene, 38: 732-49. 1938.
[18]  Zar, J.H., ed. Biostatistical Analysis. 4th ed. 1999, Prentice Hall: London, UK.
[19]  Baranyi, J. and T.A. Roberts, A dynamic approach to predicting bacterial growth in food. International Journal of Food Microbiology, 23: 277-294. 1994.
[20]  Adams, M.R. and M.O. Moss, Food Microbiology. Cambridge, United Kingdom: The Royal Society of Chemistry. 2nd ed. 2000.
[21]  Nguyen, T.T.T., et al., Effect of fermentation by amylolytic lactic acid bacteria, in process combinations, on characteristics of rice/soybean slurries: A new method for preparing high energy density complementary foods for young children. Food Chemistry, 100(2): 623-631. 2007.
[22]  Mugula, J.K., J.A. Narvhus, and T. Sørhaug, Use of starter cultures of lactic acid bacteria and yeasts in the preparation of Togwa, a Tanzanian fermented food. International Journal of Food Microbiology, 83(3): 307-318. 2003a.
[23]  Sezonov, G., D. Joseleau-Petit, and R. D'Ari, Escherichia coli Physiology in Luria-Bertani Broth. Journal of Bacteriology, 189(23): 8746-8749. 2007.
[24]  Wakil, S.M., et al., PCR-DGGE fingerprints of microbial successional changes during fermentation of cereal-legume weaning foods. African Journal of Biotechnology, 7(24): 4643-4652. 2008.
[25]  Nout, M.J.R., F.M. Rombouts, and A. Havelaar, Effect of accelerated natural lactic fermentation of infant good ingredients on some pathogenic microorganisms. International Journal of Food Microbiology, 8(4): 351-361. 1989b.