American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: http://www.sciepub.com/journal/ajfst Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2021, 9(2), 43-52
DOI: 10.12691/ajfst-9-2-3
Open AccessArticle

Changes During Nitrogen Balance of Biochemical Nutritional Parameters of Rats (Rattus Norvegicus) Fed with Different Food Formulations Containing Moringa Oleifera

Mathieu Nahounou BLEYERE1, , Baudouin Angoua KOKORE2, Adama Koffi AMARA1 and Paul Angoué YAPO1

1Department of Physiology Pharmacology and Pharmacopoeia, Nangui Abrogoua University, Abidjan, Côte d'Ivoire

2Department of Animal Biology, Peleforo Gon Coulibaly University, Korhogo, Côte d'Ivoire

Pub. Date: June 06, 2021

Cite this paper:
Mathieu Nahounou BLEYERE, Baudouin Angoua KOKORE, Adama Koffi AMARA and Paul Angoué YAPO. Changes During Nitrogen Balance of Biochemical Nutritional Parameters of Rats (Rattus Norvegicus) Fed with Different Food Formulations Containing Moringa Oleifera. American Journal of Food Science and Technology. 2021; 9(2):43-52. doi: 10.12691/ajfst-9-2-3

Abstract

Blood chemistry parameters are an efficient and reliable way to assess nutritional status. Serum contains many substances, such as proteins, enzymes, lipids and minerals. These substances which constitute the biochemical blood parameters provide information about the state of tissues and organs in the body as well as the metabolic state of the individual. Understanding the effects of a food formulation on these biochemical parameters is necessary for the vulgarisation of that formulation. The aim of this study is to explore variations in biochemical parameters in rats (Rattus norvegicus) fed with different food formulas containing Moringa oleifera during a nitrogen assessment. Rats of wistar strain were fed for 15 days with five food formulations in which Moringa oleifera leaf powder has been incorporated respectively at 0, 25, 50, 75 and 100% in partial or total substitution to soybean meal and codified L3P, L3P25, L3P50, L3P75 and L3P100. Blood samples were taken just before the experiment and two weeks of individual feeding in dry and gray tubes for the determination of blood biochemical parameters. The results indicated that only albumin and albumin/globulin ratio were significantly increased in the L3P50 rats. Regarding lipid parameters, High density lipoprotein (HDL) cholesterol showed a significant increase in all formulated foods. Aspartate Aminotransferase (ASAT) levels decreased significantly in all formulations, while the other biochemical blood nutritional parameters showed no significant difference. Our formulations based on Moringa increase blood levels of certain protein and lipid parameters, do not alter mineral levels and cause a decrease in the level of ASAT in rat.

Keywords:
Moringa oleifera food formulations biochemical parameters rat (Ratus norvegicus)

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References:

[1]  Anwar, F., Latif, S., Ashraf, M., Gilani, A.H. Moringa oleifera Lam. a food plant with multiple medicinal uses. Phytotherapy research, 21, (1), 17-25. Jan 2007.
 
[2]  Mohammed, K.A.E.F, Sarmiento-Franco, L., SantosRicalde, R., Solorio-Sanchez, J.F. The nutritional effect of Moringa oleifera fresh leaves as feed supplement on Rhode Island Red hen egg production and quality. Tropical animal health and production, 44, (5), 1035-1040. Jun 2012.
 
[3]  Aïssi, K.A., Yehouenou, P.E., Ahoyo, T.A., Fah, L., Fanou, B., Koumolou, L., et al. Evaluation of toxicological risk related to the presence of lead and cadmium in powders of leaves of Moringa oleifera Lam. marketed in Cotonou (Benin). Food and nutrition Sciences, 5, 770-778. Feb 2014.
 
[4]  Gupta, R., Patra, R.C., Saini, M., Swarup, D. Haematology and Serum Biochemistry of Chital (Axis axis) and Barking Deer (Muntiacus muntjak) Reared in Semi-Captivity, Veterinary Research Communications, 31, 801-808. Feb 2007.
 
[5]  Sato, S., Parr, E.B., Devlin, B.L., Hawley, J.A., SassoneCorsi, P. Human metabolomics reveal daily variations under nutritional challenges specific to serum and skeletal muscle. Molecular metabolism, 16, 1-11. Oct 2018.
 
[6]  Atchade, G.S.T., Mensah, S.E.P., Houndonougbo, M.F., Attakpa, S.E. Paramètres biochimiques sériques des lapins (Oryctolagus cuniculus Linnaeus, 1758) nourris avec des aliments à base de ressources alimentaires d’Afrique de l’Ouest: Synthèse bibliographique, J. Appl. Biosci., 138, 14060-14071. Dec 2019.
 
[7]  Lyimo, M.H., Nyagwegwe, S., Mnkeni, A.P. Investigations on the effect of traditional food processing, preservation and storage methods on vegetable nutrients: A case study in Tanzania. Plant Foods for Human Nutrition, 41, 53-57. Jan 1991.
 
[8]  Adrian, R.J., Particle-Imaging Techniques for Experimental Fluid Mechanics. Annual Review of Fluid Mechanics, 23, 261-304. Jan 1991.
 
[9]  Tenter, A.M., Heckeroth, A.R., Weiss, L.M. Toxoplasma gondii: from animals to humans. Int J Parasitol, 30, (12-13), 1217-1258. Nov 2000. doi: 10.1016/s0020-7519(00)00124-7. Erratum in: Int J Parasitol 31, (2), 217-220. PMID: 11113252; PMCID: PMC3109627.
 
[10]  Descat, E., Montaudon, M., Latrabe, V., Surcin, B., Morales, P., Laurent, F. MR imaging of myocardial haematoma after blunt chest injury. Eur Radiol, 12, S174-S176. Dec 2002.
 
[11]  Amara, A.K., Goze, B.N., Yapo, P.A0. Blood parameters in rats (Rattus norvegicus) fed a new food (L3P) produced in laboratory of Physiology, Pharmacology and Pharmacopoeia SDRP Journal of Cellular and Molecular Physiology, 2(2):144-153. Sep 2018.
 
[12]  Tete–Benissan, A., Quashie, M.L.A , Lawsonevi, K., Kokou, K., Messanvi, G. Récupération nutritionnelle chez les sujets malnutris VIH positifs et VIH négatifs après utilisation de feuilles de Moringa oleifera Lam. Journal of Animal &Plant Sciences, 15, (2), 2184-2199. Oct 2012.
 
[13]  Atawodi, S.E., Atawodi, J.C., Idakwo, G.A., Pfundstein, B., Haubner, R., Wurtele, G., Bartsch, H. and Owen, R.W. Evaluation of the Polyphenol Content and Antioxidant Properties of Methanol Extracts of the Leaves, Stem, and Root Barks of Moringa oleifera Lam. Journal of Medicinal Food. 13(3): 710-716. Jun 2010.
 
[14]  Moyo, B., Oyedemi, S., Masika, P.J., Muchenje, V. Polyphenolic content and antioxidant properties of Moringa oleifera leaf extracts and enzymatic activity of liver from goats supplemented with Moringa oleifera leaves/sunflower seed cake. Meat Science; 91(4), 441-447. Aug 2012.
 
[15]  Ali, K.M., Wonnerth, A., Huber, K., Wojta, J. Cardiovascular disease risk reduction by raising HDL cholesterol – current therapies and future opportunities. British Journal of Pharmacology, 167, 1177-1194. Nov 2012.
 
[16]  Mayes, P.A. Lipid transport and storage, in Granner, D.K., Mayes, P.A. and Rodwell, V.W., (edn), Harpers Biochemistry, 24 (New Jersey: Prentice hall) 1996; pp:254-255.
 
[17]  Jimeno, C.A., Mark Anthony, S. Effect of Malunggay (Moringa oleifera) Capsules on Lipid and Glucose Levels. Acta Medica Philippina, 47, (3), 22-27. Sep 2013.
 
[18]  Essawy, A., Beeker, H.M., Abdel-Wahhab, K.G., Sayad, O.N., Saber, S.R. Efficacy of Moringa oleifera Aqueous Extract in Inhibiting Tamoxifen®-Induced Physiological Hepatic Deterioration in Male Albino Rats. Egypt. Acad. J. Biolog. Sci., 9(2), 23-37. Jun 2017.
 
[19]  Mehta, K., Balaraman, R., Amin, A.H., Bafna, P.A., Gulati, O.D. Effect of fruits of Moringa oleifera on the lipid profile of normal and hypercholesterolaemic rabbits. J Ethnopharmacol, 86, (2-3), 191-195. Jun 2003.
 
[20]  Nwobodo, E., Ofili, J.O. Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in highfat diet fed Wister rats. J Ethnopharmacol, 69: 21-25. Jan 2000.
 
[21]  Reddy, V.P., Urooj, A., Sairam, S., Ahmed, F., Prasad, N.N. Hypocholesterolemic Effect of Moringa oleifera Polyphenols in Rats Fed High Fat-Cholesterol Diet. Mal J Nutr, 23, (2), 473-478. 2017.