American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2021, 9(2), 30-37
DOI: 10.12691/ajfst-9-2-1
Open AccessArticle

Optimization of Bacteriocin Production by Lactobacillus fermentum Strain COE20 from Fermenting Pentaclethra macrophylla Benth Using Response Surface Methodology

Onwuakor Chijioke E.1, , Ogbulie Jude N.2, Braide Wesley2, Ogbulie Tochukwu E.3, Nwokafor Chibuzo V.1 and Uchendu C.E.4

1Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria

2Department of Microbiology, School of Biological Sciences, Federal University of Technology, Owerri, Imo State, Nigeria

3Department of Biotechnology, School of Biological Sciences, Federal University of Technology, Owerri, Imo State, Nigeria

4Department of Statistics, College of Physical Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria

Pub. Date: May 07, 2021

Cite this paper:
Onwuakor Chijioke E., Ogbulie Jude N., Braide Wesley, Ogbulie Tochukwu E., Nwokafor Chibuzo V. and Uchendu C.E.. Optimization of Bacteriocin Production by Lactobacillus fermentum Strain COE20 from Fermenting Pentaclethra macrophylla Benth Using Response Surface Methodology. American Journal of Food Science and Technology. 2021; 9(2):30-37. doi: 10.12691/ajfst-9-2-1


This study evaluated the effect of varied culture conditions (Temperature, pH, and Sodium Chloride concentration) on bacteriocin production by Lactobacillus fermentum strain COE20 isolated from fermenting African oil bean seeds (Pentaclethra macrophylla Benth) using Response Surface Methodology (RSM). A Central Composite Design (CCD) was adopted with the interest of estimating the optimal conditions for its production using the response surface regression model, which estimated the linear, squared, and interactive relationship between the response variables. The Analysis of Variance (ANOVA) showed that the coefficient of determination in terms of predicted R2 was 0.8697 which was in close agreement with an adjusted R2 of 0.7393 and was accounted for by the predictors suggesting that the model was adequate. Optimal culture condition for bacteriocin production by L. fermentum strain COE20 was found at approximately 31°C, pH 5.9, 1.9% NaCl concentration at Y = 11.75mm. Y represents the response (zone of inhibition) against Staphylococcus aureus ATCC 19095 using the agar well diffusion assay method.

optimization bacteriocin Pentaclethra macrophylla response surface methodology Lactobacillus fermentum

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