American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2020, 8(6), 257-262
DOI: 10.12691/ajfst-8-6-5
Open AccessArticle

Improving the Nutritive Value and Quality Parameters of Gluten Free Bread by Using Natural and Economical Ingredients

Shereen L. Nassef1, and Hoda H. Hafez1

1Food Technology Research Institute ARC, Egypt

Pub. Date: December 30, 2020

Cite this paper:
Shereen L. Nassef and Hoda H. Hafez. Improving the Nutritive Value and Quality Parameters of Gluten Free Bread by Using Natural and Economical Ingredients. American Journal of Food Science and Technology. 2020; 8(6):257-262. doi: 10.12691/ajfst-8-6-5


Absence of gluten in gluten free bread is an important technological challenge, in this study sweet whey protein powder and fresh yogurt were added individually and together at 10 and 15 %. (W/W corn flour). Different levels of yogurt and whey protein powder were incorporated into gluten-free bread formulations. Bread quality parameters i.e. chemical, physical, textural, staling rate, water activity, sensory and economical evaluation were performed to assess the nutritional and technological qualities of the free gluten bread. Results confirmed that the yogurt and whey protein addition led to a significant increase in protein, fat, ash, crude fibres, calories, Ce, Fe and Zn content in bread while significant decrease in available carbohydrates comparing with the control. Textural profile analysis showed a decrease in hardness, gumminess and chewiness when whey protein and yogurt were added to bread formula either they added individually or together. The staling rate indicated that whey protein and fresh yogurt caused retarding in the staling rate and the whey protein bread can be kept up to 3 days with good freshness. Whey protein or yogurt addition to bread formula show a significant increase in appearance, crust colour, taste, texture, layer separation and overall score. Rising levels of supplementation with whey protein and yogurt generally caused an increase in all sensory scores of free gluten bread comparing with the control, this improvement was noticed when whey protein and yogurt were added together. Water content and water activity measurements was in the allowance range, so that water activity predicted the stability and keeping safety and quality of the prepared bread. The gluten - free bread was safe for up to 3 days for consumer. Whey protein and fresh yogurt specially when they added together improving the daily diet of celiac people, so whey protein bread and yogurt bread enhanced the nutritional value of gluten free bread such as protein, Ca, Fe and Zn, also it had good efficiency of covering the recommended daily allowances (RDA %). The cost of fortified bread with whey protein and yogurt was increased by 9.84% to 44.09 % as compared with the control cost, but that increase in the cost can be adjusted to consumer by health benefits.

gluten-free bread yogurt whey protein

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[1]  Rosell C.M., Barro F., Sousa C., Mena M.C. 2014. Cereals for developing gluten-free products and analytical tools for gluten detection. J. Cereal Sci., 59: 354-364.
[2]  Pellegrini N., and Agostoni C. 2015. Nutritional aspects of gluten-free products. J. Sci. Food Agric., 95: 2380-2385.
[3]  Capriles V.D., dos Santos F.G. and Arêas J.A.G. 2016. Gluten-free breadmaking: Improving nutritional and bioactive compounds. J. Cereal. Sci., 67: 83-91.
[4]  Calle J., Benavent-Gil Y. and Rosell C. 2020. Development of gluten free breads from Colocasia esculenta flour blended with hydrocolloids and enzymes. Food Hydrocoll. 98: 105243.
[5]  Ahlborn G.J., Pike O.A., Hendrix S.B., Hess W.M. and Huber C.S.2005. Sensory, mechanical, and microscopic evaluation of staling in low-protein and gluten-free breads. Cereal Chem., 82: 328-335.
[6]  Do Nascimento A.B., Fiates G.M.R. and dos Anjos A., Teixeira E. 2014. Gluten-free is not enough - perception and suggestions of celiac consumers. Int. J. Food Sci. Nutri., 65: 394-398.
[7]  Graça, C.; Raymundo, A. and Sousa, I. 2019. Wheat Bread with Dairy Products—Technology, Nutritional, and Sensory Properties. Appl. Sci., 9: 4101.
[8]  Sharafi, S.; Yousefi, S. and Faraji, A. 2017. Developing an innovative textural structure for semi-volume breads based on interaction of spray-dried yogurt powder and jujube polysaccharide. Int. J. Biol. Macromol., 104: 992-1002.
[9]  Prazeres, A.; Carvalho, F.; Rivas, J. 2012. Cheese whey management: A review. J. Environ. Manag. 110: 48-68.
[10]  Lappa, I.K.; Papadaki, A.; Kachrimanidou, V.; Terpou, A.; Koulougliotis, D.; Eriotou, E.; Kopsahelis, N. 2019. Cheese whey processing: Integrated biorefinery concepts and emerging food applications. 8: 347.
[11]  Alsaed, A. K. and Ahmed, R. (2010). Concentrating and drying of whey and utilization of the produced products in bakery and confectionery. A progress report, Department of Nutrition and Food Technology, Faculty of Agriculture, University of Jordan, Amman, Jordan.
[12]  Ostojic, S., Pavlovic, M., ّZivic, M., Filipovic, Z., Gorjanovic, S., Hranisavljevic´, S. and Dojcˇinovic`, M. (2005). Processing of whey from dairy industry waste, Environ Chem Lett, 3, 29-32.
[13]  Madureira, A.; Pereira, C.; Gomes, A.; Pintado, M.; Malcata, F. Bovine whey proteins - overview on their main biological properties. Food Res. Int. 2007, 40, 1197-1210.
[14]  Zahran, G. A. 2013. Production of tortilla bread from corn flour. Egyptian J. of nutrition, vol xx, 3.
[15]  AACC. (2002). Approved Method of American Association of Cereal chemists. Approved Methods the AACC published by the American Association of Cereal Chemists. 13th ed., Inc. St. Paul, Minnesota, USA.
[16]  Cadden, A. M. (1988). Moisture sorption characteristics of several food fibers. J. Food Sci. 53: 1150-1155.
[17]  Kitterman, J. S. and Rubenthalor, G. L. (1971). Assessing the quality of early generation wheat selection with the micro A.W.R.C. test. Cereal Sci., Today. 16: 313.
[18]  Johnson, J. and Harris, M. (1989). Effect of acidulants in controlling browing in cakes prepared with 100 % high fructose corn syrup of sucrose. Cereal Chem., 66: 158-161.
[19]  A.O.A.C. (2005). Official Methods of Analysis of the Association of Official Analytical Chemists. Arlington, Virginia, USA.
[20]  Fraser and Holmes (1959). Proximate analysis of wheat flour carbohydrate. In. Analysis of whole meal flour and its some of its fractions. J. of Science of Food and Agricultural, 10 (9): 506-512.
[21]  FAO/WHO 1991. Protein Quality Evaluation. Reports of a join FAO/WHO expert Consultion, Food and Agriculture Organization of the United Nations, FAO, Rome. Pp1-66.
[22]  Snedecor, G. W. and Cochran, W. G. (1980). Statistical methods 7th ed. Iowa State Univ., Press. Ames., Iowa, USA.
[23]  Lieke, E., Riemsdijk, V., Atze, V., and Flamer, R. J. 2011. The use of whey protein particles in gluten-free bread product, the effect of particle stability. Food Hydrocolloids. 25: 1744-1750.
[24]  Al-Mohtaseb, A. H., Harara, M. H., Megahey, E. K., Mcminn, W. A. and Magee, T. R. A. (2010). Moisture adsorption isotherm of microwave-baked Maderira cake. LWT-Food Sci. Tech. 43: 1042-1049.
[25]  Cook, F. K. and Johnson, B. L. (2010). Microbiological spoilage of cereal products in: Food microbiology and food safety. Sperber, W. H. Ad Doyle M.P. (ed). P223. Springer New York, USA.
[26]  Carla Graca, Anabela Raymondoand Isabel Sousa.Yogurt as an alternative Ingredient toImprove the Functional and Nuritional Properties of Gluten free Breads. Foods 2020, 9(2), 111.
[27]  Shabib, Z. A. E. (1999). Evaluation of soy bean products as affected by extrusion expeller and utilization in balady bread making. M. Sc. Thesis, Food Sci., and Tech. Dept., Fac. Of Agric., Cairo Univ.
[28]  Food and Nutrition Board, Institute of Medicine & National Academies 2004). Dietary Reference Intakes (DRIS).