American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: http://www.sciepub.com/journal/ajfst Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2020, 8(6), 233-241
DOI: 10.12691/ajfst-8-6-2
Open AccessArticle

Quality Characteristics of Probiotic Soy Yoghurts with Enzyme Hydrolyzed African Breadfruit and Rice Additives

Lucretia I. Barber1, , Chijioke M. Osuji2, Ngozika C. Onuegbu2 and Chika C. Ogueke2

1Department of Food Science and Technology, Rivers State University, Nkpolu-Oroworukwo, P.M.B 5080, Port Harcourt, Rivers State, Nigeria

2Department of Food Science and Technology, Federal University of Technology, Owerri, Imo State, Nigeria

Pub. Date: November 04, 2020

Cite this paper:
Lucretia I. Barber, Chijioke M. Osuji, Ngozika C. Onuegbu and Chika C. Ogueke. Quality Characteristics of Probiotic Soy Yoghurts with Enzyme Hydrolyzed African Breadfruit and Rice Additives. American Journal of Food Science and Technology. 2020; 8(6):233-241. doi: 10.12691/ajfst-8-6-2

Abstract

Effect of enzyme hydrolyzed African breadfruit (HABF) and culture type on the physicochemical and nutrient composition of soy yoghurt sweetened with rice syrup was evaluated. The effect of the HABF on the final counts of the cultures; Bifidobacterium bifidum (ATCC 11883) and Lactobacillus acidophilus in mono- and co-cultured soy yoghurt was also determined. African breadfruit (ABF) flour was hydrolysed with a mixture of cell wall degrading enzymes: Xylanase [endo-1,4-] and [endo-1,3(4)-] Beta-glucanase (UltraflowmaxR). HABF was added at concentrations of 0 - 5 % into soymilk containing 25 % hydrolysed rice syrup and then pasteurized at 80 °C for 30 min in a water bath. Probiotic B. bifidum and L. acidophillus as mono- and co-cultures were separately inoculated into the soy-HABF milk and fermented at 42°C for 6 - 8 h. Samples were analyzed using standard methods. The pH (4.46 - 4.30) and syneresis index (32.35 - 25.00) decreased significantly (P ≤ 0.05) with increase in HABF concentration for the cultures, while TTA (0.62 - 0.93 % lactic acid) and viscosity (1.20 - 1.84 Pa.s-1) increased significantly (P ≤ 0.05). No significant (P ≤ 0.05) effect on moisture (85.79 - 89.16 %), crude protein (3.44 - 3.75 %) and crude fat (1.24 - 1.58 %) content of the soy yoghurt was observed. The ash (0.17 - 0.50 %), crude fibre (0.24 - 0.47 %) and carbohydrate (7.10 - 8.53 %) varied significantly (P ≤ 0.05) amongst the cultures with HABF concentrations. B. bifidum and L.acidophillus monoculture counts ranged from 7.36 - 7.69 and 7.16 - 8.49 Log10 CFU/ml respectively, and 6.52 - 7.66 and 7.79 - 8.92 Log10 CFU/ml respectively in co-culture fermentation. Viable cells were > 106 recommended for probiotic products. This work therefore reveals the possibility of the production of probiotic yoghurt with soymilk and at least 4% HABF inclusion and rice syrup as sweetner.

Keywords:
enzyme hydrolysis African breadfruit soy yoghurt probiotics bifidobacterium bifidum lactobacillus acidophillus

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Fisberg, M. and Machado, R., History of yogurt and current patterns of consumption. Nutrition Reviews, 73(S1): 4-7. 2015
 
[2]  Erkaya, T. and Sengul, M., Comparison of volatile compounds in yoghurt made from cow, buffaloes, ewes and goat milk. International Journal of Dairy Technology, 64 :(2) 240-246. 2011.
 
[3]  Al-Saleh, A.A., Metwalli, A.A. and Ismail, E.A., Physicochemical properties of probiotic frozen yoghurt made from camel milk. International Journal of Dairy Technology, 64(4): 445-562. 2011.
 
[4]  Makunjuola, O.M., Production and quality evaluation of soy-corn yoghurt. Advance Journal of Food science and Technology, 4: 130-134. 2012.
 
[5]  Smid and Kleerebezem, Production of aroma compounds in lactic fermentations. Annual Review of Food Science and Technology 5: 313-326. 2014.
 
[6]  Nielsen, J.W. and Gilliland, S.E., The lactose hydrolyzing enzyme from Lactobacillus acidophilus. Cultured Dairy Products Journal, 27: 20-28. 1992.
 
[7]  Donkor, O.N., Nilmini, S.L.I., Stolic, P., Vasiljevic, T. and Shah, N.P., Survival and activity of selected probiotic organism in set-type yoghurt during cold storage. International Dairy Journal, 17: 657-665. 2007.
 
[8]  Wzoreck, W., Bonin, S. and Koskowska, J., Attempt to Obtain Beverage Containing Viable Lactic Acid Bacteria and Estimation of their Survivability at the Selected Temperatures. Act Technologia Alimenteria, 2: 47-56. 2003.
 
[9]  Iwe, M.O., The Science and Technology of Soybean. pp. 145. Rejoint Communication Services Ltd, 2003
 
[10]  Chun, J., Kim, J. and Kim, J., Enrichment of isoflavones aglycones in soymilk by fermentation with single and mixed cultures of Streptococcus infantarius 12 and Weissella sp. Food Chemistry, 109:278-284. 2008.
 
[11]  Estevez, A.M., Mejia, J., Figuerola, F. and Escobar, B., Effect of Solid Content and Sugar Combinations on the Quality of Soymilk Based Yoghurt. Journal of Food Processing and Preservation, 34:87-97. 2010.
 
[12]  Champagne, C.P., Raymond, Y. and Tompkins T.A., The Determination of Viable Counts in Probiotic Cultures Microencapsulated by Spray Coating. Food Microbiology. 27: 1104-11. 2010.
 
[13]  Nsofor, L.M., Nsofor, O.N. and Nwachukwu, K.E., Soy-yoghurt Starter Culture Development from Fermented Tropical Vegetables. Journal of the Science of Food and Agriculture, 60: 515-518. 1992.
 
[14]  Kaur, H., Mishra, H.N. and Kumar, P., Textural properties of mango soy fortified probiotic yoghurt: optimisation of inoculum level of yoghurt and probiotic culture. International Journal of Food Science and Technology, 44(2): 415-424. 2009.
 
[15]  Appiah, F., Oduro, I. and Ellis, W.O., Pasting Properties of Treculia Africana Seed Flour in Ghana and Production of Breakfast Cereal. Agricultural Biology Journal, 2: 325-329. 2011.
 
[16]  Ifediba, D.I. and Ozoh, C.N. Effects of Storage on Physicochemical Properties and Microbiological Qualities of African Breadfruit-Corn Yoghurt. European Scientific Journal, 14(6) 172-191. 2018.
 
[17]  Kunze, W. (1996). Technology Brewing and Malting. Int. Ed .VLB, Berlin Germany.
 
[18]  Osuji, C.M. and Nwosu, J.C., Effect of Soybean Sprouting and Beta glucanase treatment of Wet Milled Soybeans on the Chemical Property of Soymilk from Different Variety of Soybeans. Nigerian Food Journal 29: (1) 94-102. 2011.
 
[19]  Champagne, C.P., Green-Johnson, J., Raymond, Y., Barrette, J., Buckley, N., Selection of probiotic bacteria for the fermentation of a soy beverage in combination with Streptococcus thermophilus. Food Research International, 42: 612-621. 2009.
 
[20]  AOAC, Official Methods of Analysis of Association of Official Analytical Chemists. 18th Edition, 2010. Washington, DC.
 
[21]  Unal, E. and Akalin, A.S., Antioxidant and angiotensin-converting enzyme inhibitory activity of yoghurt fortified with sodium calcium caseinate or whey protein concentrate. Dairy Science and Technology 92(6): 627-639. 2012.
 
[22]  Park, D.J., Oh, S., Ku, K., H., Mok, C., Kim, S.H. and Imm, J.Y., Characteristics of Yoghurt-like Products Prepared from the combination of Skim milk and Soymilk Containing Saccharified- Rice Solution. Journal of Food Sciences and Nutrition. 56(1): 23-34. 2005.
 
[23]  Niyibituronsa, M., Onyango, A.N., Gaidashova, S., Imathiu, S., De Boevre, M., Leenknecht, D., Neirnck, E., De Saeger, S., Vermei,r P. and Raes, K., The growth of different probiotic microorganisms in soymilk from different soybean varieties and their effects on anti-oxidant activity and oligosaccharide content. Journal of Food Research, 8(1): 41-51. 2019.
 
[24]  Garro, M.S., De Valdez, G.F. and De Giori, G.S., Temperature effect on the biological activity of Bifidobacterium longum CRL 849 and Lactobacillus fermentum CRL 251 in pure and mixed cultures grown in soymilk. Food Microbiology, 21, 511-518. 2004.
 
[25]  Obadina, A., Akinola, O.J, Shittu, T. and Adegoke, B., Effect of Natural Fermentation on the Chemical and Nutritional Composition of Fermented Soymilk Nono. Nigerian Food Journal, 31(2):91-97. 2019.
 
[26]  Niamah, A.F. and Al-Manhel, A.J., Gum Arabic uses as Probiotic in Yoghurt Production and Study effects on Physical, Chemical Properties and Survivability of Probiotic bacteria during cold Storage. World Applied Sciences Journal 34:1190-1196. 2016.
 
[27]  Wang, J., Guo, Z., Zhang, Q., Yan, L., Chen, W., X.-M. Liu, X-M. and Zhang, H-P., Fermentation characteristics and transit tolerance of probiotic Lactobacillus casei Zhang in soymilk and bovine milk during storage. Journal of Dairy Science, 92: 92:2468-2476. 2008.
 
[28]  Chou, C.C. and Hou, J. W., Growth of Bifidobacteria in soymilk and their survival in the fermented drink during storage. International Journal of Food Microbiology, 56: 113-121. 2000.
 
[29]  Sodini, I., Remeuf, F., Haddad, S. and Corrieu, G., The relative effect of milk base, starter and process on yoghurt texture -A review. Critical Reviews in Food Science and Nutrition, 44: 113-137. 2004.
 
[30]  Mishra, S. and Mishra, H N., Effect of Synbiotic Interaction of Fructooligosaccharide and Probiotics on the Acidification Profile, Textural and Rheological Characteristics of Fermented Soy Milk. Food and Bioprocess Technology, 6: 3166-3176. 2013.
 
[31]  Sengul, M., Erkay, T., Sengul, M., Yildiz, H., The effect of adding sour cherry pulp into yoghurt on the physicochemical properties, phenolic content and antioxidant activity during storage. International Journal of Dairy Science, 65(3): 429-436. 2012.
 
[32]  Vareltzis, P., Adamopoulos, K., Stavrakakis, E., Stefanakis, A. and Goula, A.M., Approaches to minimise yoghurt syneresis in simulated tzatziki sauce preparation. International Journal of Dairy Technology, 69(2) 191-199. 2016
 
[33]  Kucukoner, E. and Tarakci, Z., Influence of Different Fruit Additives on some Properties of Shirred Yoghurt During Storage. Journal of Agricultural Science 13(2) 97-101. 2003
 
[34]  Tusda, H. and Miyamoto, T., Production of exopolysaccharide by Lactobacillus plantarum and the prebiotic activity of the exopolysaccharide. Food Science and Technology Research, 16: 87-92. 2010.
 
[35]  Ifediba, D.I. and Nwafor, E.C., Nutritional and sensory evaluation of African breadfruit corn yoghurt. African Journal of Food Science, 12(4): 73-79. 2018.
 
[36]  Júnior, S.I., Tavanob, O.L., Demontec, A., Rossic, E.A. and Pintoc, R.A., Nutritional Evaluation of soy yoghurt in comparison to soymilk and commercial milk yoghurt. Effect of fermentation on soy protein. Acta Alimentaria, 41: 443-450. 2012.
 
[37]  Amanze, K.O. and Amanze, J.O.Quality evaluation from cow milk, soymilk and cow/soymilk. Journal of Research in National Development. 9: 44-47.2011.
 
[38]  Akubor1, P.I and Badifu, G.I.O (2004). Proximate composition, functional properties and baking potential of African breadfruit kernels and wheat flour blends. International Journal of Food Science and Technology. 39: 223-229
 
[39]  Ndife, J., Idoko, F. and Garba, R., Production and quality assessment of functional yoghurt enriched with coconut. International Journal of Nutrition and Food Sciences. 3(6): 545-550. 2019.
 
[40]  Oyeyipo, O.O., Iwuji, C.A. and Nworgu U.G., Sensory Evaluation of a Vegetable Yoghurt-like Product from the African Breadfruit (Treculia africana) Seeds. International Journal of Food Research 2: 11-16. 2012.
 
[41]  Osabor, V.N., Ogar, D.A., Okafor, P.C. and Egbung, G.E., Profiles of the African Breadfruit (Treculia africana). Pakistan Journal of Nutriton. 8: 1005-1008. 2009.
 
[42]  Olubamiwa, A.O. and Kolakpo, A.L., Evaluation of Nutritional composition and acceptability of Soy-Coconut Milk- Based Yoghurt Fermented with different Starter Cultures. Food Global Science Books, 2:65-69. 2008.
 
[43]  Osundahunsi, O.F., Amosu, D., Ifesan. B.O.T., Quality evaluation and acceptability of soy-yoghurt with different colours and fruit flaviours. American Journal of Food Technology, 2(4) 273-280. 2007.
 
[44]  Niamah, A.F. and Al-Manhel, A.J., Gum Arabic uses as Probiotic in Yoghurt Production and Study effects on Physical, Chemical Properties and Survivability of Probiotic bacteria during cold Storage. World Applied Sciences Journal 34: 1190-1196. 2016.
 
[45]  Kurman, J.A. and Rasic, J.L., The health potential of products containing Bifidobacteria. In: Therapeutic properties of fermented milks (edited by Robin-son, R.K.,). Pp. 117-158. 1991. London: Elsevier Applied Food Sciences
 
[46]  Martensson O., Oste, R. and Holst, O., The effect of yoghurt culture on the survival of probiotic bacteria in oat-based, non-dairy products. Food Research International, 35: 775-784. 2002.
 
[47]  Akalin, A.S., Fenderya, S. and Akbulut, N., Viability and Activity of Bifidobacteria in yoghurt containing fructooligosaccharide during refrigerated storage. International Journal of Food Science and Technology 39: 613-621. 2004.