American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2020, 8(4), 136-146
DOI: 10.12691/ajfst-8-4-2
Open AccessArticle

Influence of pH, Total Acidity and Technological Processes of Preparation of babenda “leafy vegetables and cereal sauce” on Pesticide Residues

Tarnagda Bakary1, 2, Zongo Oumarou1, Traoré Korotimi3, Sourabié-Ouattara Pane Bernadette2, Konaté Soumaila2, Drabo Koine Maxime4, Traoré Yves1 and Savadogo Aly1,

1LABIA/CRSBAN/Department of Biochemistry and Microbiology, UFR-SVT, University Joseph KI-ZERBO, Burkina Faso, 03 BP 7021, Ouagadougou, Burkina-Faso

2National Public Health Laboratory / Quality Control and Applied Nutrition Department, 09 BP 24 Ouaga 09, Ouagadougou, Burkina Faso

3Department of Food Technology / National Center for Scientific and Technological Research, Ouagadougou, Burkina Faso, Cameroon

4Department of Public Hygiene / Ministry of Health / 09 BP 24 Ouaga 09, Ouagadougou, Burkina Faso

Pub. Date: June 15, 2020

Cite this paper:
Tarnagda Bakary, Zongo Oumarou, Traoré Korotimi, Sourabié-Ouattara Pane Bernadette, Konaté Soumaila, Drabo Koine Maxime, Traoré Yves and Savadogo Aly. Influence of pH, Total Acidity and Technological Processes of Preparation of babenda “leafy vegetables and cereal sauce” on Pesticide Residues. American Journal of Food Science and Technology. 2020; 8(4):136-146. doi: 10.12691/ajfst-8-4-2


The uncontrolled use of pesticides on leafy vegetables has been frequently reported in recent years. The aim of this study was to evaluate the influence of pH, total acidity and various technological processes for the preparation of babenda on pesticide residues contained in leafy vegetable. The "babenda" is a Burkinabè dish consisting mainly of Hibiscus sabdariffa, Amaranthus hybridus and Cleome gynandra coarsely cut from steamed rice. The Quick, Easy Cheap Effective Rugged Safe Method (QuEChERS) and Gas Chromatography with Microelectron Capture Detector (GC-μECD) were used. Six pesticides were detected in 180 samples of leafy vegetables and cereal sauce (babenda) collected in 5 cities of Burkina Faso. Results showed that 56% of the samples contained residues among which 41.66% contained concentrations above the LMR and 25% contained multiple pesticides. There were found a correlation between pH and dieldrin content. Compared with the diuron with an average value of 0.0625-0.0969, there is no correlation between this molecule and the pH (5.86) whose acidity varies between 0.04 - 0.10. The pH has an influence on the cypermethrin and the diuron. However acidity has an effect on the degradation of Lamda-cyhalothrines, 2, 4 DDT, dieldrin, heptachlor. For treatments of 150°C and 300°C, organochlorine pesticides are little or no degradation. There is a considerable reduction as the temperature increases. Only lindane, heptachlor, aldrin, alpa-endosulfan and 2, 4 DDT which have undergone total destruction from 250 and 300°C. Significant reductions were observed in all treatments applied. Future research needs to focus on the integration of food safety parameters into actual applied research at the level of babenda producers.

Pesticide Babenda technological processes QuEChERS LMR Burkina Faso

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[1]  Hama-Ba, F., C. Parkouda, R. Kamga, A. Tenkouano, B. Diawara, 2017. "Disponibilite, modes et frequence de consommation des legumes traditionnels Africains dans quatre localités du Burkina Faso a diverses activités de maraichage: Ouagadougou, Koubri, Loumbila, Kongoussi". Afr. J. Food Agric. Nutr. Dev 17(1): 11552-11570.
[2]  Tarnagda, B., H. Cissé, J.U. Muandze Nzambe, P.B. Ouattara-Sourabié, Y. Itsiembou, F. Guira, C. Zongo, Y. Traoré, A. Savadogo, 2019.Technologie de production du « babenda» un aliment à base de céréale et de légumes feuilles au Burkina Faso. Am J In Res Appl Sci 13 p. ISSN 2429-5396.
[3]  K. Congo, 2013. Risques sanitaires associes à l’utilisation de pesticides autour de petites retenues: cas du barrage de loumbila. In Mémoire master 2iE. p. 68.
[4]  Son, D. I. Somda, A. Legreve, B .Schiffers, 2017. Practical plant health of the tomato producers of Burkina Faso and health risks and the environment. Book Agriculture 26, 25005.
[5]  Tarnagda, B., A. Tankoano, F. Tapsoba, P.B. Sourabe, H.O. Abdoullahi, A.O. Djbrine, K.M. Drabo, Y. Traoré, A. Savadogo, 2017. Evaluation des pratiques agricoles des légumes feuilles: le cas des utilisations des pesticides et des intrants chimiques sur les sites maraîchers de Ouagadougou, Burkina Faso. J Appl Biosci 117, 11658-11668.
[6]  Bempah, C.K., A. Donkor, P.O. Yeboah, B. Dubey and P. Osei-Fosu, 2011. A preliminary assessment of consumer’s exposure to organochlorine pesticides in fruits and vegetables and the potential health risk in Accra Metropolis, Ghana. Food Chem 128: 1058–1065.
[7]  Tarnagda, B., F. Guira, P.B. Sourabié, O. Zongo, F. Tapsoba, C. Zongo, K.M. Drabo, Y. Traoré, A. Savadogo, 2019. Evaluation of heavy metals and pesticides contents in market-gardening products sold in some principal markets of Ouagadougou (Burkina Faso). J Microbiol Biotechnol Food Sci 2018; 8 (4) 1026-1034.
[8]  Machekano, W.M., M.M. Brighton and N. Casper, 2019.Cabbage or ‘pesticide’ on the platter? Chemical analysis reveals multiple and excessive residues in African vegetable markets. Inter J. Food Contam 6:2.
[9]  Palmquist, K., J. Salatas and A. Fairbrother, 2012. “Pyrethroid Insecticides: use, environmental fate, and ecotoxicology”, in Perveen F (Ed) Insecticides-Advances in Integrated Pest Management, Exponent. In Tech, pp. 251-278.
[10]  Tomer, V., J. Kaur, S. Balwinder, S.R. Takkar, 2014. Efficacy of processing treatments on cypermethrin residues in okra (Abelmoschus esculentus)", Nutrition & Food Sci Toxicol 48, (1) 1-6, Vol. 44 Iss 6 pp. 545-553.
[11]  Chavarri, M.J., A. Herrera & A. Arino, 2004. Pesticide residues in field-sprayed and processed fruits and vegetables. J. Sci of Food and Agri 84, (10) 1253 -1259.
[12]  E.R. Elkins, 1989. Effect of Commercial Processing on Pesticide-Residues in Selected Fruits and Vegetables. JAOAC 72, (3) 533-535.
[13]  Dejonckheere, W., W. Steurbaut, S. Drieghe, R. Verstraeten and H. Braeckman, 1996a. Monitoring of pesticide residues in fresh vegetables, fruits, and other selected food. IJAOAC 79, 97-110.
[14]  Schattenberg, H.J., P.W.Geno, J.P. Hsu, W.G. Fry and R.P. Parke, 1996. Effect of household preparation on levels of pesticide residues in produce. IJAOA 79, (6) 1447-1453.
[15]  Krol W.J., T.L. Arsenault, H.M. Pylypiw and M.J.I. Mattina, 2000. Reduction of pesticide residues on produce by rinsing. J. Agr. Food Chem 48, (10) 4666-4670.
[16]  Kaushik, G., S. Satya and S.N. Naik, 2009. Food processing a tool to pesticide residue dissipation - a review. Food Resear Inter 42, 26-40.
[17]  A.V. Holden And K. Marsden, 2000. Single stage clean-up of animal tissue extracts for organochlorine residue analysis. J. Chromatog, 14, 481.
[18]  Farrow, R. P., F.C. Lamb, R. W. Cook, J.R. Kimball And E. R. Elkins, 1968. Removal of DDT, malathion and carbaryl from tomatoes by commercial and home preparative methods. J. Agr. Fd Chem 16, 65.
[19]  Hemphill, D.D., R.E. Baldwin, A. Deguzman And H.K. Deloach, 1967. Effects of washing, trimming and cooking on levels of DDT and derivatives in green beans. J. Agr. Fd Chem 15, 290.
[20]  Elkins, E.R., F.C. Lamb, C.R.P. Farrow, R.W. Cook, M. Kawai and J.R. Kimball, 1968. Removal of DDT, malathion and carbaryl from green beans by commercial and home preparative procedures. J. Agr. Food Chem 16, 962.
[21]  Lamb, F.C., R.P. Farrow, E.R. Elkins, R.W. Cook and J.R. Kimball. 1968. Behavior of DDT in potatoes du ring commercial and home preparation. J. Agr. Food Chem 16, 272.
[22]  Rasmusssen, R.R., M.E.A. Poulsen and H.C.B. Hansen, 2003. Distribution of multiple pesticide residues in apple segments after home processing. Food Addit Contam 20, (11) 1044-1063.
[23]  Radwan, M.A., M.M. BU-Elamayem, M.H. Shiboob and Bdel-AAL, 2005. Residual behaviour of profenofos on some field-grown vegetables and its removal using various washing solutions and household processing. Food Chem. Toxicol 43, (4) 553-557.
[24]  Walia, S., P. Boora, B. Kumar, 2010. Effect of Processing on Dislodging of Cypermethrin Residues on Brinjal. Bull Environ Contam Toxicol 84:465-468.
[25]  Angioni, A., M. Schirra, V. L. Garau, M. Melis, Tuberoso, C. I. G. & Cabras, P. Burchat, C. S., Ripley, B. D., Leishman, P. D., Ritcey, G. M., Kakuda, Y. & G.R. Stephenson, 2007. The distribution of nine pesticides between the juice and pulp of carrots and tomatoes after home processing. Food Addit and Contam 15, (1) 61-71.
[26]  Duhan, A., B. Kumari & R Gulati, 2010. Effect of Household Processing on Fenazaquin. Residues in Okra Fruits. Bulletin of Envir Contam and Toxicol 84, (2) 217-220.
[27]  Chun, O.K. & H.G. Kang, 2003. Estimation of risks of pesticide exposure, by food intake, to Koreans. Food and Chem Toxicol 41, (8) 1063-1076.
[28]  Zhang, Z.Y., X.J Liu and X.Y Hong, 2007. Effects of home preparation on pesticide residues in cabbage. Food Control 18, (12) 1484-1487.
[29]  Gamet-Payrastre, L. and C. Lukowicz, 2017. Les effets des mélanges de pesticides. Impact of pesticides mixtures. Cahiers de Nutrition et de Diététique. Volume 52, Issue 5, November 2017, Pages 234-238.
[30]  Farrow, R.P., F.C Lamb, R.W. Cook, J.R Kimball and Elkins ER (1968) Removal of DDT, malathion and carbaryl from tomatoes by commercial and home preparative methods. J Agr Food Chem; 16, 65.
[31]  P.J. Fellows, 2000. Food processing technology, Second ed. Boca Raton, Woodhead Publishing Limited and CRC Press LLC.
[32]  Comission Directive, 2002/63/EC, 2002. Establishing community methods of sampling for the official control of pesticide residues in and on products of plant and animal origin and repealing directive 79/700/EEC. OJEC 187, 30-43.
[33]  Nougadère, A., V. Sirot, A. Kadar, A. Fastier, E. Truchot, C. Vergnet, F. Homet, J. Bayle, P. Gros, J.C. Leblanc, 2012. Total diet study on pesticide residues in France: levels in food as consumed and chronic dietary risk to consumers. Environ Int 45:135-50.
[34]  Mwanja, M., C. Jacobs, A. R. Mbewe, S.N. Munyinda, 2017. Assessment of pesticide residue levels among locally produced fruits and vegetables in Monze District, Zambia. Int J Food Contam 4:11.
[35]  Islam, M., S. Shamsad, 2009. Assessment of irrigationwater quality of Bogra district in Bangladesh. Bangladesh Journal of Agricultural Research, 34, 4, 507-608.
[36]  D. Firestone, 2008. American oil Chemistes’ Society official Methodes and Recommended Practices. AOCS Press. Urbana, IL.
[37]  Qozowicka, B., M. Jankowska, P. Kaczynski, 2012. Pesticide residues in Brassica vegetables and exposure assessment of consumers. Food Control, 561-575.
[38]  M. Anastassiades, 2005. QuEChERS: A mini-multiresidue method for the analysis of pesticide residues in low-fat products, Chemisches und Veterinaruntersuchung Sant Stuttgart, JAOAC Int. 12p.
[39]  Amoah, P., P. Drechsel, R.C. Abaidoo, W. J. Ntow, 2006. Pesticide and pathogen contamination of vegetables in Ghana’s urban markets. Arch Environl Contam Toxicol 50:1-6.
[40]  Holland, P.T., D. Hamilton, B. Ohlin and M.W Skidmore1994). Effects of storage and processing on pesticide residues in plant products. Pure and Applied Chem 66, (2) 335-356.
[41]  Osei-Fosu, P, A. Donkor, C. Ziwu, B. Dubey, R. Kingsford-Adaboh, I. Asante, S. Stephen Nyarko, R. Tawiah, N. Nazzah, 2017. Surveillance of pesticide residues in fruits and vegetables from Accra Metropolis markets, Ghana, 2010–2012: a case study in sub-Saharan Africa. Environ Sci Pollut Res 24:17187-205.
[42]  Islam, M.S., M.K. Ahmed, M. Habibullah-AL-Mamun, 2015. Determination of heavy metals in fish and vegetables in Bangladesh and health implications. AEHS 21, 4, 986-1006.
[43]  Anastasiades, M., D. Stajnbaher, F.J. Schenk, S. Lehotay, 2003. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and “dispersive phase solid-phase extraction for the determination of pesticide residues in produce”. J AOAC Int 86:412-31.
[44]  Food and Agriculture Organisation FAO, 1995. Fruit and vegetable processing.
[45]  E U (Europeenne Union), 2010. Maximum limits applicable to the residues of pesticides present in or on the foodstuffs and feeding stuffs of vegetable origin, OJEU 67-71.
[46]  Codex Alimentarius, 2007. Pesticide residues in food 2007. Joint FAO/WHO Meeting on Pesticide residues, Rome, p.191
[47]  E U (Europeenne Union), 2008. Maximum limits applicable to the residues of pesticides present in or on the foodstuffs and feeding stuffs of vegetable origin. OJEU 67-71.
[48]  Touré, N.K.P.K., K.E. Yoboue, A. Yao-Kouame, 2015. Evaluation of the heavy metals traces, pesticides and plasticizers in the products maraîchers (gombo, corete potagere, epinard and aubergine) in lavallee of the nieki, southeast of the Côte.d'ivoire. European Scientific Newspaper 11, 33, 14.
[49]  S.L. Byrne and S.L. Pinkerton, 2004. The effect of cooking on chlorpyrifos and 3,5,6- trichloro-2- pyridinol levels in chlorpyrifos fortified produce for use in refining dietary exposure. J Agr Food Chem 52, 7567-7573.
[50]  Codex Alimentarius, 2017. International Food Standards. Available at: Accessed 10 November, 2017
[51]  Tariq, M.I., S. Afzal, I. Hussain, N. Sultana, 2007. Pesticides exposure in Pakistan: A review. Environ Inter 33(8): 1107-22.
[52]  Kaushik, G., S. Satya and S.N. Naik, 2009. Food processing a tool to pesticide residue dissipation - a review. Food Resear Inter 42, 26-40.
[53]  López-Pérez, G.C., M. Arias-Estévez, E. López-Periago, B. Soto-González, B. CanchoGrande, J. Simal Gándara, 2006. Dynamics of pesticides in potato crops. J. Agri Food Chem 54(5), 1797-1803.
[54]  I, Macharia, 2015. Pesticides and health in vegetable production in Kenya. BioMed Res Int.
[55]  B. Lazowicka, 2015. Health risk for children and adults consuming apples with pesticide residues. Sci The Total Environ 502:184-98.
[56]  B.M. Keikotlhaile, P Spanoghe and Steurbaut W (2010a) Behaviour of pesticide residues in selected vegetables after domestic steaming. 62 nd Crop Prot. May 18, Ghent University, Belgium.
[57]  M. Kroger, 1968. Effect of various physical treatments on certains organochlorine hydrocarbon insecticides found in milk fat. J. Dairy Sci 51, 194-198.
[58]  M. Hekmati and R.L. Bradley, 1971. Degradation of organochlorine pesticides with hydrogen peroxide. Milchwissenschait 26, 224.
[59]  Keikotlhaile, B.M., P. Spanoghe and W. Steurbaut, 2010b. Effects of food processing on pesticide residues in fruits and vegetables: A meta-analysis approach. Food and Chem 48, 1-6.