Determination of Lipase Activities and Lipid Peroxidation Level of Fermented Oil Bean Seeds (Ugba, Pentaclethra macrophylla), Castor Oil Seeds (Ogiri, Ricinus communis) and Millet Seeds (Kunu, Eleusine coracana)

Ugwu Chidiebere Emmanuel^1, , Nduka C², Maduka Hugh Clifford Chima^{1, 2}, Okpogba Aloysius Ngozi¹ and Ogueche Peter Nnamdi¹

¹Department of Human Biochemistry, Faculty of Basic Medical Sciences Nnamdi Azikiwe University Awka, Nnewi Campus, Anambra State Nigeria

²Department of Biochemistry College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State Nigeria

Cite this paper:
Ugwu Chidiebere Emmanuel, Nduka C, Maduka Hugh Clifford Chima, Okpogba Aloysius Ngozi and Ogueche Peter Nnamdi. Determination of Lipase Activities and Lipid Peroxidation Level of Fermented Oil Bean Seeds (Ugba, Pentaclethra macrophylla), Castor Oil Seeds (Ogiri, Ricinus communis) and Millet Seeds (Kunu, Eleusine coracana). American Journal of Food Science and Technology. 2019; 7(5):157-160. doi: 10.12691/ajfst-7-5-4

Abstract

Lipases are glycerol ester hydrolases which hydrolyze esther linkages of glycerides at water-oil interface. The activities of lipase in fermented oil bean seeds (ugba), fermented castor oil seeds (ogiri) and kunu were determined with respect to the levels of free fatty acids produced from each sample. The samples were prepared by local adoption of processing oil bean and castor seeds. The seeds were cooked (100°C) in cooking pot for 4hr, chopped into pieces, washed with clean tap water and covered in a stainless pot and allowed to ferment at room temperature. The lipid peroxidation and lipase activity were also determined on the fermented products using standard methods. The total free fatty acid determined were 349± 7, 1026± 5 and 94± 5mg/ml for ugba, ogiri and kunu respectively. The level of lipid hydroperoxide concentrations of fermented ugba, ogiri and kunu were (2.5 ±0.3) ×10^-4, (1.57 ±0.05) ×10^-4 and (4.5 ±0.3) × 10^-4 mg/ml malondialdehyde respectively. It was observed that the higher the peroxidation or concentration of malondialdehyde in a given sample, the least the lipase activity as determined by the level of free fatty acids. The findings of this research indicate that fermented ugba, ogiri and kunu could be good sources of lipase for industrial application.

Keywords:
lipase activity malondialdehyde free fatty acid fermentation lipid peroxidation

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References:

[1]	Achi OK. (2005). Traditional fermented protein condiment in Nigeria. African .Journal of Biotechnology. 4(12):1612-1621.
[2]	Falegan A. (2014). Enzymatic and haematological changes in rats (Rattus norvegecus) fed with defated ogiri (fermented Citrullus vulgaris) and melon seeds. Journal of Natural Sciences Research. 4 (17): 133-140.
[3]	Ibeabuchi JC, Olawuni IA, Iheagwara MC, Ojukwu M and Ofoedu CE. (2014). Microbiological evaluation of ‘iru’ and ‘ogiri-isi’ used as food condiments. IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT). 8(8): 45-50.
[4]	Ogungbemi K, Alejo AO, Ilesanmil FF, Ishola DT, Afolabi AA, Oyelakin MO, Zaka KO, Ajanwachukwu CN. (2017). Sensory, shelf-life and nutritional evaluation of kunu (Nigeria non-alcoholic beverage) produced from different grains. International Journal of Research Studies in Agricultural Sciences (IJRSAS).3(9): 20-25.
[5]	Abulude FO, Ogunkoya MO, Oni VA. (2006). Mineral composition, shelf-life and sensory attributes of fortified “kunuzaki” beverage. Acta Sci. Pol. Technol Aliment. 5(1): 155-162.
[6]	Onuorah SI, Adesiyun AA, Adekeye JO. (2012). Survival and multiplication of Staphylococcus aureus and Echerichia coli in Nigerian ceral drink(Kunu zaki). Effect of spices, pH and temperature. Journal of Food Agriculture. 1: 169-173.
[7]	Fapohunda SO and Adeware A. (2012). Microbial load and keeping quality of kunu under various preservative regimes. Journal of Nutrition and Food Sciences. 2: 4.
[8]	Tahir F, Oyewole O. (1993). Bacteriological studies of kunuzaki. Nigeria Journal of Microbiology. 9: 47-53.
[9]	Adeyemi IA, Umar S. 1994. Effect of methods of manufacture on quality characterization of “kunuzaki”, a millet based beverages. Nigeria Food Journal. 12: 33-40.
[10]	Bankole MO, Akpan I, Atanda OO. (1999). The anti-microbial effect of spices in kunutsamiya. Nigeria Journal of Microbiol. 13: 95-97.
[11]	Gureeva NV. (2009). Pancreatic lipase activation, inhibition, and relationship to peroxidation of lipids. Pharmaceutical Chemistry Journal. 43(11): 619-624.
[12]	Gitlesen T, Bauer M and Adlercreutz P. (1997). Adsorption of lipase on polypropylene powder. Biochimica et Biophysical Acta. 1345: 188-196.
[13]	Gulati R, ISAR Jasmine, Kumar V, Parmar VS, Sexana R.K. (2005). Production of a novel alkaline lipase by Fusarium globulosum using Neem oil and its applications. Pure and Applied Chemistry. 77(1): 251-262.
[14]	Gombert AK, Pinto AL, Castilho LR, Freira DMG. (1999). Lipase by Penicillium restrictum in solid state fermentation using Babassu oil cake as substrate. Process Biochemistry. 35(1):85-90.
[15]	Nipon S, Revathi D, Sheelu G, YamunaRani K, Sridhar S, Mehtab V, Suman C. (2018). Recent advances on sources and industrial applications of lipases. Biotechnology Progress. 34(1): 5-28.
[16]	Odunfa SA. (1985). Biochemical changes in fermenting African locust bean (Parkia bigiobosa) during iru fermentation. Journal of Food Technology. 20: 295-303.
[17]	Odunfa SA and Oyeyiola GE. (1985). Microbiological study of the fermentation of ‘ugba’- a Nigerian indigenous fermented food flavor. Journal of Plant Foods. 6: 155-163.
[18]	Maduka HCC. (2008). Effect of time course administration of bergenin an isolate of Sacogloths gabonensis, Nigerian alcoholic beverage additive on lipid peroxidation and natural antioxidant defenses during normal biological oxidation in weaning rats in vivo. Nigerian Journal of Botany. 21(1): 109-121.
[19]	Maduka, H.C.C., Chukwu, N.C., Ugwu, C.E., Dike, C.C., Okpogba, A.N., Ogueche, P,N and Maduka, A.A. (2014). Assessment of commercial bottled table and sachet water commonly consumed in Federal University of Technology, Owerri (FUTO), Imo State, Nigeria using microbiological indices. IOSR Journal of Dental and Medical Sciences. 13,(1):86-89.
[20]	Maduka HCC, Mohammed IH, Okpogba AN, Ugwu CE, Dike CC, Maduka AA, Ogueche PN, Gadaka MA. (2014). Biochemical assessment of some common commercial fruit juices consumed in Maiduguri Metropolis, Borno State Nigeria. Journal of Natural Sciences Research. 4(10): 94-98.
[21]	Maduka HCC, Ugwu CE, Dike CC, Aguoru CU, Okpogba AN, Ogueche PN, Maduka AA, Gadaka MA, Alike SO, Okonkwo CO. (2014).Assessment of some commercial fruit juices commonly consumed in Federal University of Agriculture Makurdi, Benue State Nigeria, for lipid peroxidation intermediates and antioxidant vitamin. Advances in Life Science and Technology. 24: 49-52.
[22]	Steinkraus KH. (2002). Fermentations in world food processing. Comprehensive Reviews in Food Science and Food Safety. 1: 23-32.