American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: http://www.sciepub.com/journal/ajfst Editor-in-chief: Hyo Choi
Open Access
Journal Browser
Go
American Journal of Food Science and Technology. 2019, 7(3), 71-78
DOI: 10.12691/ajfst-7-3-1
Open AccessArticle

Combined Effect of UV-C and Ozone on Bioactive Compounds and Microbiological Quality of Fresh-Cut Rocket Leaves

Diego R. Gutiérrez1, 2 and Silvia Del C. Rodríguez1, 2,

1Centro de Investigaciones en Biofisica Aplicada y Alimentos (CIBAAL). CONICET-Universidad Nacional de Santiago del Estero (UNSE). RN 9, Km 1125. El Zanjón. (CP 4206)-Santiago del Estero. Argentina

2Instituto de Ciencia y Tecnología de Alimentos (ICyTA-FAyA-UNSE). Belgrano (S) Nº 1912-Santiago del Estero, Argentina

Pub. Date: March 22, 2019

Cite this paper:
Diego R. Gutiérrez and Silvia Del C. Rodríguez. Combined Effect of UV-C and Ozone on Bioactive Compounds and Microbiological Quality of Fresh-Cut Rocket Leaves. American Journal of Food Science and Technology. 2019; 7(3):71-78. doi: 10.12691/ajfst-7-3-1

Abstract

The separate application of UV-C (25 kJ/m2, 380 s) and O3 gaseous (2.5 mg/L for 10 min) treatments and of their combination (25 kJ/m2 UV-C with 2.5 mg/L O3) were studied to evaluate the effect of combined treatments of UV-C and O3 on microbial counts, bioactive profile and sensory changes of fresh-cut rocket leaves throughout shelf life to determine whether these treatment have additive or synergistic. The separate application of UV-C and O3 and of their combination UV-C + O3 did not affect the sensorial quality, total chlorophyll contents, phenolic compound nor antioxidant capacity of fresh-cut rocket leaves. However, these treatments controlled better the growth of epiphytic microbes than untreated samples. The UV-C treatment had better effect at reducing microorganisms present and not significant differences were found as to the combined treatment. Therefore, applying the combined treatment UV-C + O3 had neither synergistic nor additive effect in the extension of the fresh-cut rocket leaves shelf life.

Keywords:
UV-C ozone synergism bioactive compounds quality rocket

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Teoh L. S., Lasekan O., Adzahan N. M., and Hashim N. (2016). The effect of combinations of UV-C exposure with ascorbate and calcium chloride dips on the enzymatic activities and total phenolic content of minimally processed yam slices. Postharvest Biology and Technology, 120: 138-144.
 
[2]  Huang H., Ge Z., Limwachiranon J., Li L., Li W., and Luo Z. (2017). UV-C treatment affects browning and starch metabolism of minimally processed lily bulb. Postharvest Biology and Technology, 128: 105-111.
 
[3]  Cocetta G., Baldassarre V., Spinardi A., and Ferrante A. (2014). Effect of cutting on ascorbic acid oxidation and recycling in fresh-cut baby spinach (Spinacia oleracea L.) leaves. Postharvest Biology and Technology, 88: 8-16.
 
[4]  Char C., Silveira A.C., Inestroza-Lizardo C., Hinojosa A., Machuca A., and Escalona V. H. (2012). Effect of noble gas-enriched atmospheres on the overall quality of ready-to-eat arugula salads. Postharvest Biology and Technology, 73: 50-55.
 
[5]  Manchali S., Chidambara Murthy K.N., and Patil B.S. (2012). Crucial facts about health benefits of popular cruciferous vegetables. Journal of Functional Foods, 4: 94-106.
 
[6]  Oliveira M., Abadias M., Usall J., Torres R., Teixidó N., and Viñas I. (2015). Application of modified atmosphere packaging as a safety approach to fresh-cut fruits and vegetables-A review. Trends Food Science Technology, 46: 13-26.
 
[7]  Hinojosa A., Gatica I., Bustamante A., Cárdenas D., and Escalona V. (2015). Effect of the combined treatment of UV-C light and modified atmosphere packaging on the inactivation of Escherichia coli inoculated watercress. Journal Food Processing and Preservation, 39: 1525-1533.
 
[8]  Lu Y., Zhang J., Wang X., Lin Q., Liu W., Xie X., Wang Z., and Guan W. (2016). Effects of UV-C irradiation on the physiological and antioxidant responses of button mushrooms (Agaricus bisporus) during storage. International Journal Food Science and Technology, 51: 1502-1508.
 
[9]  Rodoni L.M., Concellón A.I., Chaves A.R., and Vicente A.R. (2012). Use of UV-C treatments to maintain quality and extend the shelf life of green fresh-cut bell pepper (Capsicum annuum L.). Journal of Food Science, 77: 632-639.
 
[10]  Choi I.L., Yoo T.J., and Kang H.M. (2015). UV-C treatments enhance antioxidant activity, retain quality and microbial safety of fresh-cut paprika in MA Storage. Hortic Environ Biotechnology, 56: 324-329.
 
[11]  George D.S., Razali Z., Santhirasegaram V., and Somasundram C. (2015). Effects of ultraviolet light (UV-C) and heat treatment on the quality of fresh-cut Chokanan mango and Josephine pineapple. Journal of Food Science, 80: 426-434.
 
[12]  Cetin E.S. (2014). Induction of secondary metabolite production by UV-C radiation in Vitis vinifera L. Öküzgözü callus cultures. Biological Research, 47: 1-7.
 
[13]  Tarek A.R., Rasco B.A., and Sablani S.S. (2016). Ultraviolet-C light sanitization of English cucumber (Cucumis sativus) packaged in polyethylene film. Journal of Food Science, 81: 1419-1430.
 
[14]  Gogo E.O., Opiyo A.M., Hassenberg K., Ulrichs C., and Huyskens-Keil S. (2017). Postharvest UV-C treatment for extending shelf life and improving nutritional quality of African indigenous leafy vegetables. Postharvest Biology and Technology, 129: 107-117.
 
[15]  Jagadeesh S.L., Charles M.T., Gariepy Y., Goyette B., Raghavan G.S., and Vigneault C. (2011). Influence of postharvest UV-C hormesis on the bioactive components of tomato during post-treatment handling. Food Bioprocess Technology, 4: 1463-1472.
 
[16]  Bravo S., García-Alonso J., Martín-Pozuelo G., Gómez V., Santaella M., and Navarro-González I. (2012). The influence of post-harvest UV-C hormesis nolycopene-carotene, and phenolic content and antioxidant activity of breaker tomatoes. Food Research International, 49: 296-302.
 
[17]  Park M.H., and Kim J.G. (2015). Low-dose UV-C irradiation reduces the microbial population and preserves antioxidant levels in peeled garlic (Allium sativum L.) during storage. Postharvest Biology and Technology, 100: 109-112.
 
[18]  Ong M.K., Ali A., Alderson P.G., and Forney C.F. (2014). Effect of different concentrations of ozone on physiological changes associated to gas exchange, fruit ripening, fruit surface quality and defense-related enzymes levels in papaya fruit during ambient storage. Scientia Horticulturae, 179: 163-169.
 
[19]  US-FDA, United States Food and Drug Administration. (2001). “Secondary direct food additives permitted in food for human consumption. Federal Register, Rules and Regulations, June 26, Volume: 66, Number: 123, Sec., 173.368 Ozone, Final Rule.
 
[20]  Karaca H., and Velioglu Y.S. (2014). Effects of ozone treatments on microbial quality and some chemical properties of lettuce, spinach, and parsley. Postharvest Biology and Technology, 88: 46-53.
 
[21]  Minas I.S., Karaoglanidis G.S., Manganaris G.A., and Vasilakakis M. (2010). Effect of ozone application during cold storage of kiwifruit on the development of stem-end rot caused by Botrytis cinerea. Postharvest Biology and Technology, 58(3): 203-210.
 
[22]  Botondi R., Moscetti R., and Massantini R. (2016). A comparative study on the effectiveness of ozonated water and peracetic acid in the storability of packaged fresh-cut melon. Journal Food Processing and Preservation, 53: 2352-2360.
 
[23]  Liu C., Ma T., Hu W., Tian M., and Sun L. (2016). Effects of aqueous ozone treatments on microbial load reduction and shelf life extension of fresh-cut apple. International Journal Food Science and Technology, 51: 1099-1109.
 
[24]  Alothman M., Kaur B., Fazilah A., Bhat R., and. Karim A.A. (2010). Ozone-induced changes of antioxidant capacity of fresh-cut tropical fruits. Innovative Food Science and Emerging Technology, 11: 666-671.
 
[25]  Sachadyn-Król M., Materska M., Chilczuk B., Karaś M., Jakubczyk A., Perucka I., and Jackowska I. (2016). Ozone-induced changes in the content of bioactive compounds and enzyme activity during storage of pepper fruits. Food Chemistry, 211: 59-67.
 
[26]  Gutiérrez D.R., Chaves A.R., and Rodriguez S.C. (2016). Use of UV-C and gaseous ozone as sanitizing agents for keeping the quality of fresh-cut rocket (Eruca sativa Mill.). Journal Food Processing and Preservation, 00: 1-13.
 
[27]  Martínez-Hernández G.B., Artés-Hernández F., Gómez P.A., Formica A.C., and Artés F. (2013). Combination of electrolysed water, UV-C and superatmospheric O2 packaging for improving fresh-cut broccoli quality. Postharvest Biology and Technology, 76: 125-134.
 
[28]  Gutiérrez D.R., and Rodríguez S.C. (2017). Combined effect of UV-C and modified atmosphere packaging for keeping antioxidant compounds and extend to shelf-life of fresh-cut rocket leaves. International Journal of New Technology and Research (IJNTR), 03: 62-68.
 
[29]  Gutiérrez D.R., Char C., Escalona V.H., Chaves A.R., and. Rodríguez S.C. (2015). Application of UV-C radiation in the conservation of minimally processed rocket (Eruca sativa Mill.). Journal Food Processing and Preservation, 39(6): 3117-3127.
 
[30]  Lichtenthaler H.K. (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymology, 148: 350-382.
 
[31]  Singleton V.L., Orthofer R., and Lamuela-Raventós R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Method Enzymology, 299:152-153.
 
[32]  Brand-Williams W., Cuvelier M.E., and Berset C. (1995). Use of the free radical method to evaluate antioxidant activity. LWT-Food Science Technology, 28: 25-30.
 
[33]  Artés-Hernández F., Robles P.A., Gómez P.A., Tomás-Callejas A., and Artés F. (2010). Low UV-C illumination for keeping overall quality of fresh-cut watermelon. Postharvest Biology and Technology, 55: 114-120.
 
[34]  Tzortzakis N., Borland A., Singleton I., and Barnes J. (2007). Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit. Postharvest Biology and Technology, 45: 317-325.
 
[35]  Tomás-Callejas A., Otón M., Artés F., and Artés-Hernández F. (2012). Combined effect of UV-C pretreatment and high oxygen packaging for keeping the quality of fresh-cut Tatsoi baby leaves. Innovative Food Science Emerging Technology, 14: 115-121.
 
[36]  Olmez H., and Akbas M.Y. (2009). Optimization of ozone treatment of fresh-cut green leaf lettuce. Journal of Food Engineering, 90: 487-494.
 
[37]  Ali A., Ong M.K., and Forney C.F. (2014) Effect of ozone pre-conditioning on quality and antioxidant capacity of papaya fruit during ambient storage. Food Chemistry, 142: 19-26.
 
[38]  Jemni M., Gómez P.A., Souza M., Chaira N., Ferchichi A., Otón M., and Artés, F. (2014). Combined effect of UV-C, ozone and electrolyzed water for keeping overall quality of date palm. LWT-Food Science Technology, 59(2): 649-655,
 
[39]  Martínez-Hernández G.B., Gómez P.A., Pradas I., Artés F., and Artés-Hernández F. (2011). Moderate UV-C pretreatment as a quality enhancement tool in fresh-cut Bimi broccoli. Postharvest Biology and Technology, 62: 327-337.
 
[40]  Manzocco L., Da Pieve S., and Maifreni M. (2011). Impact of UV-C light on safety and quality of fresh-cut melon. Innovative Food Science Emerging Technology, 12: 13-17.
 
[41]  Martínez-Hernández G. B., Navarro-Rico J., Gómez P.A., Otón M., Artés F., and Artés-Hernández F. (2015). Combined sustainable sanitising treatments to reduce Escherichia coli and Salmonella enteritidis growth on fresh-cut kailan-hybrid broccoli. Food Control, 47: 312-317.
 
[42]  Yeoh W.K., Ali A., and Forney C.F. (2014). Effects of ozone on major antioxidants and microbial populations of fresh-cut papaya. Postharvest Biology and Technology, 89: 56-58.
 
[43]  Horvitz S., and Cantalejo M.J. (2008). The effects of gaseous ozone and chlorine on quality and shelf life of minimally processed red pepper. ISHS Acta Horticulturae, 877: 583-589.
 
[44]  Cazzaniga S., Bressan M., Carbonera D., Agostini A., and Dall’Osto L. (2016). Differential roles of carotenes and xanthophylls in photosystem I photoprotection. Biochemistry, 55: 3636-3649.
 
[45]  Guerrero R. F., Puertas B., Jiménez M. J., Cacho J., and Cantos-Villar E. (2010). Monitoring the process to obtain red wine enriched in resveratrol and piceatannol without quality loss. Food Chemistry, 122: 195-202.
 
[46]  Li S.T., Lasekan O., Adzahan N.M., and Hashim N. (2016). The effect of combinations of UV-C exposure with ascorbate and calcium chloride dips on the enzymatic activities and total phenolic content of minimally processed yam slices. Postharvest Biology and Technology, 120: 138-144.
 
[47]  Glowacz M., and Rees D. (2016). Exposure to ozone reduces postharvest quality loss in red and green chilli peppers. Food Chemistry, 210: 305-310.
 
[48]  Glowacz M., Colgan R., and Rees D. (2015). Influence of continuous exposure to gaseous ozone on the quality of red bell peppers, cucumbers and zucchini. Postharvest Biology and Technology, 99: 1-8.
 
[49]  Jin P., Yao D., Xu F., Wang H., and Zheng Y. (2015). Effect of light on quality and bioactive compounds in postharvest broccoli florets. Food Chemistry, 172: 705-709.
 
[50]  Wu X., Guan W., Yan R., Lei J., Xu L., and Wang Z. (2016). Effects of UV-C on antioxidant activity, total phenolics and main phenolic compounds of the melanin biosynthesis pathway in different tissues of button mushroom. Postharvest Biology and Technology, 118: 51-58.