American Journal of Food and Nutrition
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American Journal of Food and Nutrition. 2016, 4(5), 115-126
DOI: 10.12691/ajfn-4-5-1
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Calcium Exchange Rates in Rat Kidney Tubule Cells Affected by Insulin and pH

B.W. Brazier1,

1Bendigo Kangan Institute, Bendigo, Victoria, Australia

Pub. Date: July 11, 2016

Cite this paper:
B.W. Brazier. Calcium Exchange Rates in Rat Kidney Tubule Cells Affected by Insulin and pH. American Journal of Food and Nutrition. 2016; 4(5):115-126. doi: 10.12691/ajfn-4-5-1


Calcium exchange rates in rat kidney tubule are measured by measuring the loss of Ca45 isotopes from tubule cells when the tubules are placed in Ca45 free media that was adjusted to different pH sand when different additives of sulphate, acetate, glutamate and /or insulin were included. The exchange rates showed decrease with low pH and glutamine but increased with addition of acetate and insulin. The effect of insulin is considered in relation to its observed effect on protein induced postprandial calciuria.

hypercalciuria insulin calcium exchange rates gluconeogenesis ammoniogenisis

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