American Journal of Food and Nutrition
ISSN (Print): 2374-1155 ISSN (Online): 2374-1163 Website: Editor-in-chief: Mihalis Panagiotidis
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American Journal of Food and Nutrition. 2016, 4(1), 20-29
DOI: 10.12691/ajfn-4-1-3
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Effect of Insulin Hypercalciuric Effect of High Protein Diets

B.W. Brazier1,

1Bendigo Kangan Institute, Bendigo, Victoria, Australia

Pub. Date: April 25, 2016

Cite this paper:
B.W. Brazier. Effect of Insulin Hypercalciuric Effect of High Protein Diets. American Journal of Food and Nutrition. 2016; 4(1):20-29. doi: 10.12691/ajfn-4-1-3


Postprandial calciuric responses to high and low protein meals is examined and changes in urinary Ammonia. Urea, pH, creatinine, phosphate are compared with serum calcium, creatinine, urea, protein and insulin. Group result show that the average postprandial calciuria increase was greater after the high protein meals compared to low protein meals. But there was considerable variation between subjects. However the increase in calciuria in every case showed a plateau effect during the time of maximum insulinemia Examination of individual results show that the individuals with less insulinemia exhibited greatest hypercalciuria and those with greater insulinemia show less calciuria and even one individual with an exaggerated insulinemia response showed reduced hypercalciuria following the high protein meal. Discussion is provided regarding the significance of the protein induced hypercalciuria and possible mechanisms for it cause. The result indicate that plasma insulin had a significant effect on calciuria.

hypercalciuria protein insulin

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[1]  Abelow, B.J., Holford, T.R and lnsogna, KL. (1992) 'Cross-cultural association between dietary animal protein and hip fracture: a hypothesis', Calcif.Tissue Int., 50(1): 14.
[2]  Kaneko, K, Masaki, U., Aikyo, M., Yabuki, K, Haga, A, Matoba, c., Sasaki, H. and Koike, G. (1990) 'Urinary calcium and calcium balance in young women affected by high protein diet of soy protein isolate and adding sulfur containing amino acids and/or potassium', J.Nut.Sci.Vitaminol., Tokyo, 36(2): 105-16.
[3]  Fernandez-Repollet, E., Van Loon, P. and Martinez-Maldonado, M. (1989) 'Renal and systemic effects of short-term high protein feeding in normal rats', Am.J.Med.Sci., 297(6): 348-454.
[4]  Funaba, M., Yano, H. and Kawashima, R (1989) 'Effects of high protein diet and sodium bicarbonate supplementation on calcium metabolism in rats', J.Nur.Sci.Vitaminol., Tokyo, 35(5): 495-503.
[5]  Schneider, W. and Menden, E. (1988) 'The effect of long term increased protein administration on mineral metabolism and kidney function in the rat. Il. Kidney function and bone mineralization', Z-Ernahrungswiss., 27(3): 186-200.
[6]  Allen, L.H., Oddoye, E.A and Margen, S. (1979), 'Protein-induced hypercalciuria: a longer term study', American Journal of Clinical Nutrition, 32: 742-49.
[7]  Hegsted, M. and Linkswiler, H.M. (1981) 'Long-term effects of level of protein intake on calcium metabolism in young adult women', J.Nutr., 111(2): 244-5l.
[8]  Zemel, M.B., Schuette, S.A, Hegsted, M. and :~inkswiler, H.M. (1981) 'Role of the sulfur- containing amino acids in protein-induced hypercalciuria in men', J.Nut., 111(3): 545- 52.
[9]  Lutz, J. and Linkswiler, H.M. (1981) 'Calcium metabolism in postmenopausal and osteoporotic women consuming two levels of dietary protein', Am.J.Clin.Nut., 34(10); 2178-86.
[10]  Schuette, S.A, Zemel, M.B. and Linkswiler, H.M. (1980) 'Studies on the mechanism of protein-induced hypercalciuria in older men and women', J.Nut., 110(2): 305-15.
[11]  Rhoades, Rand Pflanzer, R (1989) Human Physiology, Saunders, Philadelphia
[12]  Nissim, I. and States, B. (1989) 'Ammoniagenesis by cultural human renal cortical epithelial cells: study with 15N [see comments], Am.J.Physiol., 256(1 Pt 2): F187-96.
[13]  Allen, L.H., Block, G.D., Wood, Rl. and Bryce, G.F. (1981), 'The role of insulin and parathyroid hormone in the protein induced calciuria of man', Nut.Res., 1981, 1: 3-11.
[14]  Wood, RJ. and Allen, L.H. (1983) 'Evidence for insulin involvement in arginine and glucose- induced hypercalciuria in the rat', J.Nutr., 113(8): 1561-67.
[15]  Howe, J.C. (1990) 'Postprandial response of calcium metabolism in postmenopausal women to meals varying in protein level/source', Metabolism, 39(12): 1246-52.
[16]  Leidid-Buckne,G and Ziegler, R (2001) ‘Diabetes mellitus a risk for osteoporosis?’. Experimental and Clinical Endocrinology & Diabetes 109 (suppl 2): 493-514.
[17]  Osteoporosis Australia (2014),’Diabetes and osteoporosis Consumer Factsheet’, 1st Edition 08/14
[18]  Floyd, JC Jr. SS Fajans, JW.Conn, RF Knopf and J. Rull, (1966) ‘Stimulation of Insulin Secretion by Amino Acids’, J of Clinical Investigation 45 (9): 1487-1502
[19]  Scales, F.M. and Harrison, AP. (1920) 'Boric acid modification of Kjeldahl Method for crops and soils analysis', J.Ind.Eng.Chem., 12: 350-52.
[20]  Chaney, AL. and Marbach, AP. (1962) 'Modified reagents for determination of urea and ammonia', Clin.Chem., 8(2): 130-32.
[21]  Willis, J.B. (1960) 'Determination of calcium in blood serum by atomic absorption spectroscopy', Nature, 186: 249-50.
[22]  Hare, RS. (1950) 'Endogenous creatinine in serum and urine',Proc.Soc.Exp.BioI.Med., 74: 148.
[23]  Fiske, CH; Subbarow, Y. (1925) 'The colorimetric determination of phosphorus', J.Biol.Chem., 66: 373-400.
[24]  Maths worksheet (2015) ‘How to Calculate Standard Error’.
[25]  Brazier, BW., (2016a) ‘Calciuric effect of dietary protein data’
[26]  Gollaher, CR., Wood, RJ., Holl, M. and Allen, L.H. (1984) A comparison of amino acid- induced hypercalciuria in sham-operated and parathyroidectomized rats, In.Nut., 114(3) 622-6.