American Journal of Food and Nutrition
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American Journal of Food and Nutrition. 2015, 3(1), 7-11
DOI: 10.12691/ajfn-3-1-2
Open AccessArticle

Antioxidant Activities of Bambara Groundnuts as Assessed by FRAP and DPPH Assays

Nyau V.1, , Prakash S.2, Rodrigues J.3 and Farrant J.3

1Department of Food Science and Technology, University of Zambia, Lusaka, Zambia

2Department of Chemistry, University of Zambia, Lusaka, Zambia

3Department of Molecular and Cell Biology, University of Cape Town, 7701 Rondebosch, South Africa

Pub. Date: February 06, 2015

Cite this paper:
Nyau V., Prakash S., Rodrigues J. and Farrant J.. Antioxidant Activities of Bambara Groundnuts as Assessed by FRAP and DPPH Assays. American Journal of Food and Nutrition. 2015; 3(1):7-11. doi: 10.12691/ajfn-3-1-2


There is a growing interest in legumes and legume based foods because of the health claims associated with their consumption. The aim of the current study was to explore the nutraceutical potential of bambara groundnuts (Vigna subterranea L. Verdc) based on the antioxidant properties. Two market classes of bambara groundnuts (red and brown) were screened. The study employed 1,1-diphenyl-2- picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) in vitro assays to screen for antioxidant properties. Bambara groundnuts were found to possess antioxidant activities. Brown bambara groundnuts exhibited the highest DPPH free radical scavenging activity with EC50 = 347 ± 4.2 µg dried extract / ml compared to 495 ± 12 µg dried extract / ml for the red bambara groundnuts. Again FRAP derived total antioxidant power was higher in the brown (6.00 ± 0.21 mmole Fe2+ / 100 g DW) compared to (5.00 ± 0.13 mmole Fe2+ / 100 g DW) in the red. Antioxidant activities demonstrated by bambara groundnuts suggest that it could be very useful as a source of antioxidants besides the commonly consumed legumes. This in itself presents an opportunity for diversification of food resources considering that bambara groundnuts are currently very much under-utilized.

Bambara groundnuts antioxidant activities nutraceuticals

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[1]  Becker, E.M., Nissen, L.R. and Skibsted, L.H. Antioxidant evaluation protocols: Food quality or health effects. Eur. Food Res. Technol. 19, 561-571, 2004.
[2]  Benzie I.F.F. Clin. Biochem. 29, 111 116, 1996.
[3]  Bhat, R. and Karim, A.A. Exploring the nutritional potential of wild and underutilized legumes. Compr Rev Food Sci Food Saft, 8, 305–331, 2009.
[4]  Brough, S.H. and Azam-Ali, S.N. The effect of soil moisture on the proximate composition of bambara groundnuts (Vigna subterranea L. Verdc). Journal of the Science of Food and Agriculture 60, 197 – 203, 1992.
[5]  Brough, S.H., Taylor, A.J. and Azam-Ali, S.N. The potential of bambara groundnuts (Vigna subterranea) in vegetable milk production and basic protein functionality systems. Food Chemistry 47, 277 – 283, 1993.
[6]  Dobiáš, P., Pavlíková, P., Adam, M., Eisner, A., Beňová, B. and Ventura, K. Comparison of pressurised fluid and ultrasonic extraction methods for analysis of plant antioxidants and their antioxidant capacity. Cent. Eur. J. Chem. 8, 87-95, 2010.
[7]  Egoli, A. Bibliographical Review. Proceedings of the workshop on Conservation and Improvement of Bambara Groundnuts (Vigna subterranean (L.) Verdc.) 14-16 November, Harare, Zimbabwe, in: Heller J, Begemann F, Mushonga J (Eds.), pp 128-134, 1995.
[8]  FAO. A Global Mapping System for Bambara Groundnuts Production. FAO Agricultural Information Management Series. No. 1, Viale delle Terme di Caracalla, 00100 Rome, Italy, 2001
[9]  Iranshahi, M., Askari, M., Sahebkar, A. and Hadgipavlou-Litina, D. Evaluation of antioxidant, anti-inflamatory, and lipoxygenase inhibitory activities of prenlated coumarin umelliprenin. DARU J. Pharmaceut. Sci.17, 99-103, 2009.
[10]  Liu, L. Phytochemical and Pharmacological perspectives of Wheat grain and Lupin seed. PhD dissertation, Southern Cross University, 2009.
[11]  Madhavi, D.L., Singhal, R.S. and Kulkarni, P.R. Technological aspects of food antioxidants. In: Madhavi DL, Deshpande SS, Salunkhe DK Food Antioxidants: Technological, Toxicological, and Health Perspectives, Marcel Dekker, New York, pp 194-197, 1996.
[12]  Oktay, M., Gulein, I. and Kufreviolglu, I. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. Labenson-Wiss U. Technol. 36, 263 -71, 2003.
[13]  Pal, D.K., Kumar, S., Chakraborty, P. and Kumar, M.A. A study on the antioxidant activity of Semecarpus anacardium L. f. Nuts. Journal of Natural Remeady 8,160–163, 2008.
[14]  Purseglove, J.W. Tropical Crops (Dicotyledons). Longman House Burnt Mill, Harlow, Essex CM20 2JE England, 1992.
[15]  Tweneboah, C.K. Modern Agriculture in the Tropics, Food crops. Co-wood Publishers, 2000.
[16]  Villan͂o, D., Troncoso, A.M. and Parrilla, M.C. Radical scavenging ability of polyphenolic compounds towards free radicals. Talanta 71, 230–235, 2007.
[17]  Zia-Ul-Haq, M., Shahid, S.A., Ahmad, S., Qayum, M. and Rasool, N. Mineral contents and antioxidant potential of selected legumes of Pakistan. Journal of Medicinal Plants Research 6(32), 4735-4740, 2012.
[18]  Spanou, C., Stagos, D., Tousias, I., Angelis, A., Aligiannis, N., Skaltsounis, I. and Kouretas, D. Assessment of Antioxidant Activity of Extracts from Unique Greek Varieties of Leguminosae Plants Using In vitro Assays. Anticancer research 27, 3403-3410, 2007.
[19]  Xu, B.J., Yuan, S.H., Chang, S.K.C. Comparative Analyses of Phenolic Composition, Antioxidant Capacity, and Color of Cool Season Legumes and other selected food legumes. Journal of Food Science 72, 2. 2007.