American Journal of Food and Nutrition
ISSN (Print): 2374-1155 ISSN (Online): 2374-1163 Website: Editor-in-chief: Mihalis Panagiotidis
Open Access
Journal Browser
American Journal of Food and Nutrition. 2014, 2(6), 95-99
DOI: 10.12691/ajfn-2-6-1
Open AccessArticle

Determination of β-carotene by High Performance Liquid Chromatography in Six Varieties of Mango (Mangifera indica L) from Western Region of Burkina Faso

Touridomon Issa Somé1, Abdoul Karim Sakira1, and Eric Dofinizoumou Tamimi1

1Laboratory of Toxicology, Environment and Health, Doctorate School of Health, University of Ouagadougou, Ouaga 03 Burkina Faso

Pub. Date: November 24, 2014

Cite this paper:
Touridomon Issa Somé, Abdoul Karim Sakira and Eric Dofinizoumou Tamimi. Determination of β-carotene by High Performance Liquid Chromatography in Six Varieties of Mango (Mangifera indica L) from Western Region of Burkina Faso. American Journal of Food and Nutrition. 2014; 2(6):95-99. doi: 10.12691/ajfn-2-6-1


Six varieties of mangoes (Amelia, Brooks, Kent, Keitt, Lippens, Springfield)harvested from different pedological areas of the western region of Burkina Faso (Bobo-Dioulasso, Orodara, Banfora) were analyzed by high performance liquid chromatography in order to determine the β-carotene content. The study revealed a variability in β-carotene content between different varieties and regions. The Amelia variety is the richest in β-carotene content and is a good source of provitamin A.

β-carotene mangoes liquid chromatography vitamin A

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Bendech M.A, Acakpo A, V Aguayo et al (2000). Promising practices and lessons learned in the fight against vitamin A deficiency in sub-Saharan Africa. Published for the United States Agency for International Development (USAID) by the project Support for Institutionalizing Child Survival (BASICS). Arlington VA, USA.
[2]  Chen B.H, Chen Y.Y (1993). Stability of Chlorophylls and carotenoids in sweet potato leaves during microwave cooking. J Agric Food Chem. 41:1315-1320.
[3]  Department of Nutrition (2005). Further analysis of the nutritional situation in Burkina Faso. Interim report, The Ministry of Health Ouagadougou.
[4]  Hotz C, Loechl C, Lubowa A et al (2012). Introduction of β-Carotene-Rich Orange Sweet Potato in Rural Uganda Resulted in Increased Vitamin A Intakes among Children and Women and Improved Vitamin A Status among Children. J. Nutr. 142: 1871-1880.
[5]  Le Bihan G, Delpeuch F, Maire B (2002). Food, Nutrition, Public Policy. France: Institute for Development Research
[6]  MC Laren D.S, Frigg M. Sight and Life (2002). Practical Guide to Vitamin A in health and the Sickness, Second Edition. Basel Switzerland.
[7]  Mercadante A Z, Rodriguez-Amaya D B (1998). Effect of ripening, cultivar differences and processing on the carotenoid composition of mango. J Agric Food Chem. 46:128-134.
[8]  Micozzi M. S, Brown ED, Edwards B K (1992). Plasma carotenoid response to chronic intake of selected foods and i3-carotene supplements in men. Am J Clin Nutr. 55: 1120-1125.
[9]  Mills J P, Tumuhimbise G A, Jamil K M et al (2009). Sweet Potato β-Carotene Bioefficacy Is Enhanced by Dietary Fat and Not Reduced by Soluble Fiber Intake in Mongolian Gerbils. J. Nutr. 139: 44-50.
[10]  Muoki P.N, Makokha AO, Onyango CA et al (2009). Potential contribution of mangoes to reduction of vitamin A deficiency in Kenya. Ecol Food Nutr. 48: 482-98.
[11]  Nana C.P, Brouwer I.D, Zagré N-M et al (2005). Community assessment of availability, consumption, and cultural acceptability of food sources of (pro) vitamin A: Toward the development of a dietary intervention among preschool children in rural Burkina Faso. Food and Nutrition Bulletin. 26: 356-365.
[12]  Nana C.P, Brouwer I.D, Zagre N M. et al (2005). Impact of Promotion of mango and liver as sources of vitamin A for young children: a pilot study in Burkina Faso. Public Health Nutrition. 6: 808-81.
[13]  Nestel P, Bouis H.E, Meenakshi J.V, et al (2006). Biofortification of Staple Food Crops. J. Nutr. 136: 1064-1067.
[14]  Rodriguez-Amaya D B. Carotenoids and food preparation: the retention of provitamin A carotenoids in processed and Stored Prepared foods. John Snow, Inc / OMNI Project, 1997, 99 p.
[15]  Rodriguez-Amaya D B. Enhancing the carotenoid levels of foods through agriculture and food technology. Internet:
[16]  Somé I.T, Zagré M N, Kafando E.P, et al (2004). Validation of a method for determination of carotenoids by HPLC: application to the determination of carotenoid content in ten varieties of sweet potato (Ipomoea batata). C. R. Chemistry. 7: 1063-1071.
[17]  UNICEF (1998).La situation des enfants dans le monde UNICEF.146 p.
[18]  Von Braun J (2005). The world Food Situation, an Overview, Washington, DC: International Policy Research Institute
[19]  WHO / UNICEF / IVACG (1998). Vitamin A supplementation, use of supplements in the treatment and prevention of vitamin A deficiency and xerophthalmia. Second edition, Geneva.
[20]  Zagre M.N Delpeuch F, Traissac P. et al (2003). Red palm oil as a source of vitamin A for mothers and children: impact of a pilot project in Burkina Faso. Public Health Nutr. 8: 733-742.
[21]  Zeba A.N, Y Prével A M, Somé I T et al (2006). The positive impact of red palm oil in school meals on vitamin A status: study in Burkina Faso. J Nut. 5, 17.
[22]  Zagré N.M Somé I.T, Ouedraogo H (2003). Effects of ripening, drying and storage after drying on the carotenoid content of mangoes-2nd International Workshop on Food Routes of healthy nutrition. 23-28 / 11/2003 Ouagadougou.