American Journal of Food and Nutrition
ISSN (Print): 2374-1155 ISSN (Online): 2374-1163 Website: http://www.sciepub.com/journal/ajfn Editor-in-chief: Mihalis Panagiotidis
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American Journal of Food and Nutrition. 2019, 7(4), 166-172
DOI: 10.12691/ajfn-7-4-7
Open AccessArticle

Review of the Nutritional Values and Biological Activities of Ziziphus Spina-christi (Sidr) Plant Extract

Afnan H. Saaty1,

1Department of Food and Nutrition Sciences, College of Human Sciences and Design, KAU, Jeddah, Saudi Arabia

Pub. Date: December 23, 2019

Cite this paper:
Afnan H. Saaty. Review of the Nutritional Values and Biological Activities of Ziziphus Spina-christi (Sidr) Plant Extract. American Journal of Food and Nutrition. 2019; 7(4):166-172. doi: 10.12691/ajfn-7-4-7

Abstract

Background: Ziziphus spina-christi of family Rhamnaceae is a terrestrial tree often recognized as Christ's thorn jujube. The plant of Ziziphus spina-christi grows in the South and East of Asia as well as in the Middle East zone. Nearly in most of the Arabic countries, Levant, and Saudi Arabia, Ziziphus spina-christi tree is called Sidr mentioned in the Holy Quran. It contains nutritional components that render the plant one of the principal food sources. The plant possesses diverse phytochemical active compounds mostly spinanine A, β sitosterol, rutin, quercetin, betulinic, and kinetic acid. In Saudi Arabia, the Sidr tree has an important therapeutic value as each part of the tree was used to maintain a healthy life. Objective: The present review aimed to collect the different nutritional values, pharmacologic, and biological activities that were linked with the utilization of different parts of Ziziphus spina-christi extracts, which have been reported in the literature. Results: Findings collected from the previously published studies showed that Ziziphus spina-christi extract possesses many pharmacological and biological activities such as hepato, cardio and nephroprotective, antidiabetic, antimicrobial, antiparasitic, anticancer, antiasthma, and antinociceptive activities. Many of these biological activities were attributed to the antioxidant action of Ziziphus spina-christi plant extract. Conclusion: It was experimentally proven that Ziziphus spina-christ extract from the Sidr tree mentioned in the Qur'an has many nutritional values and pharmacological effects that could qualify it to be the subject of other studies in humans until it is used as a supplement or alternative treatment in the future.

Keywords:
Ziziphus spina-christi Hepatoprotective Nephroprotective anticancer antimicrobial

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References:

[1]  AngiospermPhylogeny.2012. [http://www.mobot.org/MOBOT/research/APweb/].
 
[2]  AfricanPlantDatabase.2017. [https://www.ville-ge.ch/musinfo/bd/cjb/africa/recherche.php]
 
[3]  Yossef, H., Khedr, A., Mahran, M., “Hepatoprotective and antioxidant effects of Zizyphus spina-christi fruits on carbon tetrachloride induced hepatotoxicity in rats”, Nature and Science. 9.1-7. 2011.
 
[4]  Saied, A.S., Gebauer, J., Hammer, K., Buerkert, A., “Ziziphus spina-christi (L.) Willd.: a multipurpose fruit tree”, Genetic Resources and Crop Evolution. 55(7). 929-937. 2008.
 
[5]  Adzu, B., Amos, S., Dzarma, S., Wambebe, C., Gamaniel, K., “Effect of Zizyphus spina-christi Willd aqueous extract on the central nervous system in mice”, Journal of ethnopharmacology. 79(1). 13-16. 2002.
 
[6]  Michel, C.G., Nesseem, D.I., Ismail, M.F., “Anti-diabetic activity and stability study of the formulated leaf extract of Zizyphus spina-christi (L.) Willd with the influence of seasonal variation”, Journal of ethnopharmacology. 133(1). 53-62. 2011.
 
[7]  Sameera, N., Mandakini, B., “Investigations into the antibacterial activity of Ziziphus mauritiana Lam. and Ziziphus xylopyra (Retz.) Willd”, International Food Research Journal. 22(2). 849.2015.
 
[8]  Khafagi, I., Zakaria, A., Dewedar, A., El-Zahdany, K., “A voyage in the world of plants as mentioned in the Holy Quran”, International Journal of Botany. 2(3). 242-251. 2006.
 
[9]  Asgarpanah, J., Haghighat, E., “Phytochemistry and pharmacologic properties of Ziziphus spina christi (L.) Willd”, African journal of pharmacy and pharmacology. 6(31). 2332-2339. 2012.
 
[10]  Temerk, H., Salem, W., Sayed, W., Hassan, F., “Antibacterial effect of phytochemial extracts from Ziziphus-spina christi against some pathogenic bacteria”, Egyptian Journal of Botany. 57(3). 595-604. 2017.
 
[11]  Hamza, M., Garba, A., Ma’Aruf, S., “Evaluating the Medicinal and Nutritional Benefits of Nigerian Edible Fruits of Ziziphus jujube and Ziziphus spina-christi Plants”. 2015.
 
[12]  Nour, A.M., Ali, A., Ahmed, A.-H., “A chemical study of Zizyphus spina-christi (Nabag) fruits grown in Sudan”, Tropical science. 27(4). 271-273. 1987.
 
[13]  Duke, J.A.: Handbook of Medicinal Herbs, Second edn: CRC Press; 2002.
 
[14]  Berry-Koch, A., Moench, R., Hakewill, P., Dualeh, M., “Alleviation of nutritional deficiency diseases in refugees”, Food and nutrition bulletin. 12(2). 1-7. 1990.
 
[15]  Eromosele, I.C., Eromosele, C.O., Kuzhkuzha, D.M., “Evaluation of mineral elements and ascorbic acid contents in fruits of some wild plants”, Plant foods for human nutrition. 41(2). 151-154. 1991.
 
[16]  Alhakmani, F., Khan, S.A., Ahmad, A., “Determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of Zizyphus spina-christi grown in Oman”, Asian Pacific Journal of Tropical Biomedicine. 4. S656-S660. 2014.
 
[17]  Liu, J., Wang, C., Wang, Z., Zhang, C., Lu, S., Liu, J., “The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides”, Food Chemistry. 126(1). 261-269. 2011.
 
[18]  Awah, F.M., Uzoegwu, P.N., Ifeonu, P., Oyugi, J.O., Rutherford, J., Yao, X., Fehrmann, F., Fowke, K.R., Eze, M.O., “Free radical scavenging activity, phenolic contents and cytotoxicity of selected Nigerian medicinal plants”, Food Chemistry. 131(4). 1279-1286. 2012.
 
[19]  Karimi, E., Oskoueian, E., Hendra, R., Jaafar, H.Z., “Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity”, Molecules. 15(9). 6244-6256. 2010.
 
[20]  Weidner, S., Powałka, A., Karamać, M., Amarowicz, R., “Extracts of phenolic compounds from seeds of three wild grapevines—Comparison of their antioxidant activities and the content of phenolic compounds”, International journal of molecular sciences. 13(3).3444-3457. 2012.
 
[21]  Nazif, N.M., “Phytoconstituents of Zizyphus spina-christi L. fruits and their antimicrobial activity”, Food chemistry. 76(1).77-81. 2002.
 
[22]  Ghafoor, A.O., Qadir, H.K., Fakhri, N.A., “Analysis of phenolic compounds in extracts of Ziziphus spina-christi using RPHPLC method”, J Chem Pharm Res. 4(6). 3158-3163. 2012.
 
[23]  Ads, E.N., Rajendrasozhan, S., Hassan, S.I., Sharawy, S.M.S., Humaidi, J.R., “Phytochemical, antimicrobial and cytotoxic evaluation of Ziziphus spinachristi (L.) stem bark”. 2017.
 
[24]  Mohammed, G., Yesufu, H., Khan, I., Abdulrahman, F., “Effects of aqueous and ethanol extracts of the stem bark of Zizyphus spina-christi L. on isolated rabbit jejunum”, Journal of Pharmacy & Bioresources. 9(1). 14-19. 2012.
 
[25]  Huang, W., Wang, Y., Jiang, X., Sun, Y., Zhao, Z., Li, S., “Protective effect of flavonoids from Ziziphus jujuba cv. Jinsixiaozao against acetaminophen-induced liver injury by inhibiting oxidative stress and inflammation in mice”, Molecules. 22(10). 1781. 2017.
 
[26]  Soliman, S.S., Semreen, M.H., El-Keblawy, A.A., Abdullah, A., Uppuluri, P., Ibrahim, A.S., “Assessment of herbal drugs for promising anti-Candida activity”, BMC complementary and alternative medicine. 17(1). 257. 2017.
 
[27]  Abalaka, M., Daniyan, S., Mann, A., “Evaluation of the antimicrobial activities of two Ziziphus species (Ziziphus mauritiana L. and Ziziphus spinachristi L.) on some microbial pathogens”, African Journal of Pharmacy and Pharmacology. 4(4). 135-139. 2010.
 
[28]  Farmani, F., Moein, M., Amanzadeh, A., Kandelous, H.M., Ehsanpour, Z., Salimi, M., “Antiproliferative evaluation and apoptosis induction in MCF-7 cells by Ziziphus spina christi leaf extracts”, Asian Pacific journal of cancer prevention. 17(1). 315-321. 2016.
 
[29]  Kadioglu, O., Jacob, S., Bohnert, S., Naß, J., Saeed, M.E., Khalid, H., Merfort, I., Thines, E., Pommerening, T., Efferth, T., “Evaluating ancient Egyptian prescriptions today: anti-inflammatory activity of Ziziphus spina-christi”, Phytomedicine. 23(3). 293-306. 2016.
 
[30]  Abu-Taleb, A.M., El-Deeb, K., Al-Otibi, F.O., “Assessment of antifungal activity of Rumex vesicarius L. and Ziziphus spina-christi (L.) Willd. extracts against two phytopathogenic fungi”, African Journal of Microbiology Research. 5(9). 1001-1011. 2011.
 
[31]  Guo, S., Duan, J.-a., Tang, Y., Qian, Y., Zhao, J., Qian, D., Su, S., Shang, E., “Simultaneous qualitative and quantitative analysis of triterpenic acids, saponins and flavonoids in the leaves of two Ziziphus species by HPLC–PDA–MS/ELSD”, Journal of Pharmaceutical and Biomedical Analysis. 56(2). 264-270. 2011.
 
[32]  Suleiman, M.H.A., “An ethnobotanical survey of medicinal plants used by communities of Northern Kordofan region, Sudan”, Journal of ethnopharmacology. 176. 232-242. 2015.
 
[33]  Badr, S.F., El-Sherif, N.A., Shabana, I., El-Deeb, M., AL-Shinqiti, F.Y.M., “Evaluation of genetic variation, antioxidant and antibacterial activities of two Sidr varieties in Medina”, Pakistan Journal of Pharmaceutical Sciences. 33(1). 2020.
 
[34]  Karar, M.E., Quiet, L., Rezk, A., Jaiswal, R., Rehders, M., Ullrich, M., Brix, K., Kuhnert, N., “Phenolic profile and in vitro assessment of cytotoxicity and antibacterial activity of Ziziphus spina-christi leaf extracts”, Med Chem. 6(3). 143-156. 2016.
 
[35]  Dangoggo, S., Hassan, L., Sadiq, I., Manga, S., “Phytochemical analysis and antibacterial screening of leaves of Diospyros mespiliformis and Ziziphus spina-christi”, Journal of Chemical engineering. 1(1). 31-37. 2012.
 
[36]  Hadizadeh, I., Peivastegan, B., Kolahi, M., “Antifungal activity of nettle (Urtica dioica L.), colocynth (Citrullus colocynthis L. Schrad), oleander (Nerium oleander L.) and konar (Ziziphus spina-christi L.) extracts on plants pathogenic fungi”, Pakistan journal of biological sciences. 12(1). 58-63. 2009.
 
[37]  Cardozo, M.L., Ordóñez, R.M., Alberto, M.R., Zampini, I.C., Isla, M.I., “Antioxidant and anti-inflammatory activity characterization and genotoxicity evaluation of Ziziphus mistol ripe berries, exotic Argentinean fruit”, Food Research International. 44(7). 2063-2071. 2011.
 
[38]  Naik, S.R., Bhagat, S., Shah, P.D., Tare, A.A., Ingawale, D., Wadekar, R.R., “Evaluation of anti-allergic and anti-anaphylactic activity of ethanolic extract of Zizyphus jujuba fruits in rodents”, Revista Brasileira de Farmacognosia. 23(5). 811-818. 2013.
 
[39]  Hong, E.-H., Song, J.H., Kang, K.B., Sung, S.H., Ko, H.-J., Yang, H., “Anti-influenza activity of betulinic acid from Zizyphus jujuba on influenza A/PR/8 virus”, Biomolecules & therapeutics. 23(4). 345. 2015.
 
[40]  Dahiru, D., Obidoa, O., “Evaluation of the antioxidant effects of Ziziphus mauritiana lam. leaf extracts against chronic ethanol-induced hepatotoxicity in rat liver”, African Journal of Traditional, Complementary and Alternative Medicines. 5(1). 39-45. 2008.
 
[41]  Setorki, M., Hooshmandi, Z., “Neuroprotective effect of Ziziphus spina-christi on brain injury induced by transient global cerebral ischemia and reperfusion in rat”, Bangladesh Journal of Pharmacology. 12(1). 69-76. 2017.
 
[42]  Sreelatha, S., Padma, P., Umadevi, M., “Protective effects of Coriandrum sativum extracts on carbon tetrachloride-induced hepatotoxicity in rats”, Food and Chemical Toxicology. 47(4). 702-708. 2009.
 
[43]  Setorki, M., “Effect of hydro-alcoholic extract of Ziziphus spina-christi against scopolamine-induced anxiety in rats”, Bangladesh Journal of Pharmacology. 11(2). 421-427. 2016.
 
[44]  Kumarappan, C., Thilagam, E., Mandal, S.C., “Antioxidant activity of polyphenolic extracts of Ichnocarpus frutescens”, Saudi journal of biological sciences. 19(3). 349-355. 2012.
 
[45]  Kumar, S., Pandey, A.K., “Chemistry and biological activities of flavonoids: an overview”, The scientific world journal. 2013. 2013.
 
[46]  Saeed, N., Khan, M.R., Shabbir, M., “Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L”, BMC complementary and alternative medicine. 12(1). 221. 2012.
 
[47]  Hanneken, A., Lin, F.-F., Johnson, J., Maher, P., “Flavonoids protect human retinal pigment epithelial cells from oxidative-stress–induced death”, Investigative ophthalmology & visual science. 47(7). 3164-3177. 2006.
 
[48]  Kim, S.M., Kang, K., Jho, E.H., Jung, Y.J., Nho, C.W., Um, B.H., Pan, C.H., “Hepatoprotective effect of flavonoid glycosides from Lespedeza cuneata against oxidative stress induced by tert-butyl hyperoxide”, Phytotherapy Research. 25(7). 1011-1017. 2011.
 
[49]  Zhang, H., Tsao, R., “Dietary polyphenols, oxidative stress and antioxidant and anti-inflammatory effects”, Current Opinion in Food Science. 8. 33-42. 2016.
 
[50]  Hafiz, T.A., Mubaraki, M.A., Diab, M.S., Dkhil, M.A., Al-Quraishy, S., “Ameliorative role of Ziziphus spina-christi leaf extracts against hepatic injury induced by Plasmodium chabaudi infected erythrocytes”, Saudi journal of biological sciences. 26(3). 490-494. 2017.
 
[51]  Amin, A., Mahmoud-Ghoneim, D., “Zizyphus spina-christi protects against carbon tetrachloride-induced liver fibrosis in rats”, Food and Chemical Toxicology. 47(8). 2111-2119. 2009.
 
[52]  Dkhil, M.A., Al-Quraishy, S., Moneim, A.E.A., “Ziziphus spina-christi leaf extract pretreatment inhibits liver and spleen injury in a mouse model of sepsis via anti-oxidant and anti-inflammatory effects”, Inflammopharmacology. 26(3). 779-791. 2018.
 
[53]  Dkhil, M.A., Kassab, R.B., Al-Quraishy, S., Abdel-Daim, M.M., Zrieq, R., Moneim, A.E.A., “Ziziphus spina-christi (L.) leaf extract alleviates myocardial and renal dysfunction associated with sepsis in mice”, Biomedicine & Pharmacotherapy. 102.64-75. 2018.
 
[54]  Avizeh, R., Najafzadeh, H., Pourmahdi, M., Mirzaee, M., “Effect of glibenclamide and fruit extract of Zizyphus spina-christi on alloxan-induced diabetic dogs”, The Journal of Applied Research in Veterinary Medicine. 8(2). 109. 2010.
 
[55]  Solati, J., Soleimani, N., “Antihyperglycemic and antihyperlipidemic effects of Ziziphus vulgaris L. onreptozocin-induced diabetic adult male Wistar rats”, Acta diabetologica. 47(1). 219-223. 2010.
 
[56]  Vetrichelvan, T., Jegadeesan, M., “Anti-diabetic activity of alcoholic extract of Aerva lanata (L.) Juss. ex Schultes in rats”, Journal of ethnopharmacology. 80(2-3). 103-107. 2002.
 
[57]  Raju, J., Gupta, D., Rao, A.R., Yadava, P.K., Baquer, N.Z., “Trigonella foenum graecum (fenugreek) seed powder improves glucose homeostasis in alloxan diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes”, Molecular and cellular biochemistry. 224(1-2). 45-51. 2001.
 
[58]  Al-Ghamdi, A.A., Shahat, A.A., “Antioxidant, hypoglycemic and anti-diabetic activities of Ziziphus spina-christi (L) Willd (Rhamnacae) leaf extract”, Tropical Journal of Pharmaceutical Research. 16(11). 2601-2610. 2017.
 
[59]  El Rabey, H.A., Attia, E.S., Al-Seeni, M.N., Al-Sieni, A.I., Ibrahim, I.H., Meerasahib, M.F., Shaikh-Omer, A.M., Abuelgassim, A.O., “The hypolipidemic and antioxidant activity of Christ’s thorn (Ziziphus Spina-Christi) leaves powder in hypercholesterolemic male rats”, Life Science Journal. 11(10). 1010-1021. 2014.
 
[60]  Parsaeyan, N., Rezvani, M.E., “The effect of christ’s thorn (Ziziphus spina christi) leaves extract on lipid profile, lipid peroxidation and liver enzymes of diabetic rats”, Iranian Journal of Diabetes and Obesity. 6(4). 163-167. 2014.
 
[61]  Abubakar, S.M., Umar, S.A., Alexander, I., Abubakar, N., Abdulazeez, M.A., Sule, M.S., “Evaluation of hypoglycaemic, hypolipidaemic and non toxic effect of hydro-methanolic extracts of Ziziphus mauritiana, Ziziphus spina christi fruit and glibenclamide on alloxan induced diabetic rats”, Journal of Drug Delivery and Therapeutics. 8(3). 82-92. 2018.
 
[62]  Alssema, M., Dekker, J., Kuivenhoven, J., Nijpels, G., Teerlink, T., Scheffer, P., Diamant, M., Stehouwer, C., Bouter, L., Heine, R., “Elevated cholesteryl ester transfer protein concentration is associated with an increased risk for cardiovascular disease in women, but not in men, with Type 2 diabetes: the Hoorn Study”, Diabetic medicine. 24(2). 117-123. 2007.
 
[63]  Borgohain, M.P., Lahkar, M., Ahmed, S., Chowdhury, L., Kumar, S., Pant, R., Choubey, A., “Small Molecule Inhibiting Nuclear Factor-kB Ameliorates Oxidative Stress and Suppresses Renal Inflammation in Early Stage of Alloxan-Induced Diabetic Nephropathy in Rat”, Basic & Clinical Pharmacology & Toxicology. 120(5). 442-449. 2017.
 
[64]  Zhao, H., Sim, J., Shim, S., Ha, Y., Kang, S., Kim, Y., “Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction”, International Journal of obesity. 29(8). 983-990. 2005.
 
[65]  Yugarani, T., Tan, B., Teh, M., Das, N., “Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets”, Lipids. 27(3). 181-186. 1992.
 
[66]  Shahat, A.A., Pieters, L., Apers, S., Nazeif, N.M., Abdel-Azim, N.S., Berghe, D.V., Vlietinck, A.J., “Chemical and biological investigations on Zizyphus spina-christi L”, Phytotherapy Research. 15(7). 593-597. 2001.
 
[67]  Ali, A.B., Almagboul, A.Z., Mohammed, O.M., “Antimicrobial activity of fruits, leaves, seeds and stems extracts of Ziziphus spina-Christi”, Arabian Journal of Medicinal and Aromatic Plants. 1(2). 94-107. 2015.
 
[68]  Gheith, I., El-Mahmoudy, A., “Assessment of the antimicrobial potential of the hydro-methanolic extract of Sidr (Ziziphus spina-christi) plant against selected pathogens in vitro”, Life Science Journal. 15(9). 2018.
 
[69]  El-Rigal, N.S., Aly, S.A., Rizk, M., Said, A., “Effect of Ailanthus altissima and Ziziphus spina christi extracts on some hepatic marker enzymes and antioxidants in Schistosoma mansoni infected mice”, Polish journal of food and nutrition sciences. 15(2). 199-206. 2006.
 
[70]  Almeer, R.S., El-Khadragy, M.F., Abdelhabib, S., Abdel Moneim, A.E., “Ziziphus spina-christi leaf extract ameliorates schistosomiasis liver granuloma, fibrosis, and oxidative stress through downregulation of fibrinogenic signaling in mice”, PloS one. 13(10). e0204923. 2018.
 
[71]  Alzahrani, F., Al-Shaebi, E.M., Dkhil, M.A., Al-Quraishy, S., “In vivo anti-eimeria and in vitro anthelmintic activity of Ziziphus spina-christi leaf extracts”, Pakistan Journal of Zoology. 48(2). 2016.
 
[72]  Ahmadi, R., Rahimi, S., Ehteshamzad, N., “The effect of hydroalcoholic Ziziphus spina-christi leaf extract on viability of breast cancer cell line (MCF7) and evaluation of Bax and Bcl2 genes expression level”, Feyz Journal of Kashan University of Medical Sciences. 21(5). 406-412. 2017.
 
[73]  Abdel-Wahhab, M.A., Omara, E.A., Abdel-Galil, M.M., Hassan, N.S., Nada, S.A., Saeed, A., ElSayed, M.M., “Zizyphus spina-christi extract protects against aflatoxin B1-intitiated hepatic carcinogenicity”, African Journal of Traditional, Complementary, and Alternative Medicines. 4(3). 248. 2007.
 
[74]  Taha, A., El-Din, M.S., Sayed, A.A.-A., Salem, A.M., “Ziziphus spina-christi leaves methanolic extract alleviates diethylnitrosamine-induced hepatocellular carcinoma in rats”, Biochemistry and cell Biology. 2018.
 
[75]  Jafarian, A., Zolfaghari, B., Shirani, K., “Cytotoxicity of different extracts of arial parts of Ziziphus spina-christi on Hela and MDA-MB-468 tumor cells”, Advanced biomedical research. 3. 2014.
 
[76]  Rajabi, M., Garusi, S., Moezzi, H.-R., Nasir-Baghban, Z.: Zizyphus spina-christi for the management of adult asthma. In.: Eur Respiratory Soc; 2013.
 
[77]  Waggas, A.M., Al-Hasani, R.H., “Neurophysiological study on possible protective and therapeutic effects of Sidr (Zizyphus spina-christi L.) leaf extract in male albino rats treated with pentylenetetrazol”, Saudi journal of biological sciences. 17(4). 269-274. 2010.
 
[78]  Effraim, K., Osunkwo, U., Onyeyilli, P., Ngulde, A., “Preliminary investigation of the possible antinociceptive activity of aqueous leaf extract of Ziziphus spina christi (LINN) desf”, Indian journal of pharmacology. 30(4). 271. 1998.
 
[79]  Adzu, B., Amos, S., Wambebe, C., Gamaniel, K., “Antinociceptive activity of Zizyphus spina-christi root bark extract”, Fitoterapia. 72(4). 344-350. 2001.
 
[80]  Karimi, S., Hejazian, S.H., Alikhani, V., Hosseini, M., “The effects of tamoxifen on spatial and nonspatial learning and memory impairments induced by scopolamine and the brain tissues oxidative damage in ovariectomized rats”, Advanced biomedical research. 4. 2015.
 
[81]  El-Nekeety, A.A., Mohamed, S.R., Hathout, A.S., Hassan, N.S., Aly, S.E., Abdel-Wahhab, M.A., “Antioxidant properties of Thymus vulgaris oil against aflatoxin-induce oxidative stress in male rats”, Toxicon. 57(7-8). 984-991. 2011.