American Journal of Cancer Prevention
ISSN (Print): 2328-7314 ISSN (Online): 2328-7322 Website: http://www.sciepub.com/journal/ajcp Editor-in-chief: Nabil Abdel-Hamid
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American Journal of Cancer Prevention. 2018, 6(1), 9-16
DOI: 10.12691/ajcp-6-1-3
Open AccessArticle

Apple Cider Vinegar (as a Prophetic Medicine Remedy) Exerts Tissue-protective Effects in Streptozotocin-induced Diabetes in Animals

Nassar Ayoub Abdellatif Omar1, 2, Amal Nor Edeen Ahmad Allithy3, 4, Nahed Mohammed Hablas5, Ahmed Ragab Fakhreldin6, Reham Abelsalam Mariah7, Ahmed Osama Hammouda8, Mongi Ayat9, Sayed Mostafa10, Mostafa Abu-el Naga11, 12 and Salah Mohamed El Sayed13, 14,

1Department of Anatomy, Sohag Faculty of Medicine, Sohag University, Egypt

2Department of Anatomy, Al-Rayyan Medical College, Al-Madinah Al-Munawwarah, Saudi Arabia

3Department of Pathology, Sohag Faculty of Medicine, Sohag University, Egypt

4Department of Pathology, Sohag Faculty of Medicine, Al-Madinah Al-Munawwarah, Saudi Arabia

5Department of Pediatrics, Tanta University Faculty of Medicine, Tanta, Gharbia, Egypt

6Department of Pediatrics, Aswan Faculty of Medicine, Aswan University, Egypt

7Department of Medical Biochemistry, Tanta Faculty of Medicine, Tanta University, Cairo, Egypt

8Internship Program, Faculty of Medicine, Mansoura University, Egypt

9Division of Hematology, Taibah Faculty of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia

100Division of Cell Biology, Ain Shams Faculty of Medicine, Egypt

111Department of Anatomy, Faculty of Medicine, Rabigh branch, King Abul-Aziz University, Saudi Arabia

122Department of Anatomy, Faculty of Medicine, New Damietta, Al-Azhar University, Egypt

133Division of Molecular Medicine, Taibah Faculty of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia

144Division of Molecular Medicine, Sohag Faculty of Medicine, Sohag University, Egypt

Pub. Date: March 21, 2018

Cite this paper:
Nassar Ayoub Abdellatif Omar, Amal Nor Edeen Ahmad Allithy, Nahed Mohammed Hablas, Ahmed Ragab Fakhreldin, Reham Abelsalam Mariah, Ahmed Osama Hammouda, Mongi Ayat, Sayed Mostafa, Mostafa Abu-el Naga and Salah Mohamed El Sayed. Apple Cider Vinegar (as a Prophetic Medicine Remedy) Exerts Tissue-protective Effects in Streptozotocin-induced Diabetes in Animals. American Journal of Cancer Prevention. 2018; 6(1):9-16. doi: 10.12691/ajcp-6-1-3

Abstract

Background: Diabetes mellitus (DM) was reported to be related to occurrence of both biochemical and histopathological changes in hepatic tissue. DM-induced tissue damage and decreased immunity may favor carcinogenesis and tumor development in pediatric practice and adults. In this study, we aimed at investigating the hepatoprotective and tissue-protective effects of apple cider vinegar (ACV) against DM-induced hepatic derangements over a relatively long period (30 days). Effects were evaluated using streptozotocin (STZ)-induced DM in rats as an experimental model. Materials and methods: DM was experimentally caused by injecting one dose of STZ (65 mg/kg given through intraperitoneal injection. Thirty wistar rats were categorized into three experimental groups: control group, STZ-treated group and STZ plus ACV treated group 2 ml/kg BW). Animals were sacrificed 30 days post treatment. Results: Our study revealed that ACV administration induced hepatoprotective effects in diabetic rats. Biochemically, ACV reduced significantly serum glycemic values (glucose, total cholesterol, low density lipoprotein-cholesterol while high density lipoprotein cholesterol significantly increased). STZ-induced DM in rats was associated with marked fatty changes hepatocytes cytoplasm evidenced by accumulation of lipid droplets and inflammatory changes e.g. lymphocytic infiltration. That was confirmed by Electron microscopic evaluation that showed aggregations of polymorphic mitochondria, loss of mitochondrial cristae and condensed mitochondrial matrices. Hepatocytes cytoplasm showed vacuolations with a large number of variable-sized lipid droplets. Hepatocytes rough endoplasmic reticulum got distorted and fragmented into smaller stacks. ACV proved effective in reversing all these metabolic changes. ACV-treated hepatocytes exhibited minimal STZ-induced toxic effects. ACV enhanced recovery of the injured hepatocytes and normalized serum biochemical values. In prophetic medicine, Prophet Muhammad (Allah's apostle peace be upon him) strongly advised eating vinegar and considered it as the best edible as narrated in the prophetic hadith: "vinegar is the best edible". Conclusion: ACV is promising as a food additive or as a part of diet in diabetic patients particularly in early stages of DM. ACV slows down DM progress and causes hepatoprotection against the metabolic damages resulting from STZ- induced diabetes mellitus

Keywords:
Streptozotocin diabetes mellitus apple cider vinegar liver rat

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