American Journal of Cancer Prevention
ISSN (Print): 2328-7314 ISSN (Online): 2328-7322 Website: Editor-in-chief: Nabil Abdel-Hamid
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American Journal of Cancer Prevention. 2015, 3(6), 109-116
DOI: 10.12691/ajcp-3-6-2
Open AccessArticle

The Deficiency of Nuclear Proteins GATA6 and Lamin A/C as Prognostic Factor for Cervical Neoplasia

Callinice D. Capo-chichi1, 2, , Blanche Aguida1, Kathy Qi Cai3, Georges Offrin4, Vidéhouénou K Agossou5, Ambaliou Sanni1 and Xiang-Xi Xu2

1Institute of Biomedical Sciences and Applications (ISBA), Division of Molecular Biomarkers in Cancer and Nutrition (BMCN), Unit of Biochemistry and Molecular Biology (UBBM), Faculty of Sciences and Technologies (FAST), University Abomey-Calavi (UAC), Cotonou, BENIN

2Sylvester Cancer Center/ Miller School of Medicine, Department of Cell Biology, University of Miami, FL 33136, USA

3Fox Chase Cancer Center, Philadelphia, PA 19111, USA

4Hospital Mènontin, BP 160, Cotonou, BENIN

5National University Hospital (CNHU), Cotonou, BENIN

Pub. Date: October 09, 2015

Cite this paper:
Callinice D. Capo-chichi, Blanche Aguida, Kathy Qi Cai, Georges Offrin, Vidéhouénou K Agossou, Ambaliou Sanni and Xiang-Xi Xu. The Deficiency of Nuclear Proteins GATA6 and Lamin A/C as Prognostic Factor for Cervical Neoplasia. American Journal of Cancer Prevention. 2015; 3(6):109-116. doi: 10.12691/ajcp-3-6-2


BACKGROUND: Cervical cancer is a deadly disease for women worldwide and could be prevented if early molecular screening was performed ahead of cytology. In most neoplastic lesions the expression of nuclear proteins GATA6 and/or lamin A/C is deficient. OBJECTIVE: To analyze GATA6 and lamin A/C in archived cervical epithelial tissues and in cells derived from cervical uterine smears (CUS). METHODS: Immunofluorescence was used to analyze GATA6 and lamin A/C in epithelial cell lines. Immunohistochemistry was used to analyze GATA6 and lamin A/C expression in normal and neoplastic archived cervical tissues. Immunoblotting was used to analyze GATA6 and lamin A/C in cells of CUS obtained freshly from 40 selected women (age 20-45 years) who were attending routine gynecological checkup in Hospital Mènontin (Cotonou, BENIN). Colposcopy was used to visualize the vaginal tract and the uterine-cervix junction. This study is approved by our Institutional Research Ethic Committee and by the Ministry of Health in BENIN. RESULTS: Our data shows that the expression of GATA6 and lamin A/C is altered prior to cervical neoplastic lesions. The nuclear biomarkers GATA6 and lamin A/C are absent in 13% of CUS samples. Colposcopic examination performed after two years on 15 women lacking GATA6 and/or lamin A/C shows that 20% of them have developed cervical neoplastic lesions. CONCLUSION: These preliminary results suggest that the deficiency of GATA6 and lamin A/C in CUS could be used as biomarkers to contribute to the identification of women at risk of developing cervical lesions. However further studies are required for cancer prevention.

nuclear proteins GATA6 lamin A/C; cervical neoplasia cancer prevention

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