American Journal of Cancer Prevention
ISSN (Print): 2328-7314 ISSN (Online): 2328-7322 Website: http://www.sciepub.com/journal/ajcp Editor-in-chief: Nabil Abdel-Hamid
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American Journal of Cancer Prevention. 2015, 3(2), 45-50
DOI: 10.12691/ajcp-3-2-4
Open AccessArticle

Anti-proliferative, Cytotoxicity and Anti-oxidant Activity of Juglans regia Extract

Tajamul Islam Shah1, , Ekta Sharma2 and Gowhar Ahmad Shah3

1Department of Research, Jawaharlal Nehru Cancer Hospital and Research Centre, Bhopal, India

2Department of Botany, Benazeer College of science, Bhopal, India

3Department of Zoology, Benazeer College of Science and Commerce Bhopal, India

Pub. Date: April 20, 2015

Cite this paper:
Tajamul Islam Shah, Ekta Sharma and Gowhar Ahmad Shah. Anti-proliferative, Cytotoxicity and Anti-oxidant Activity of Juglans regia Extract. American Journal of Cancer Prevention. 2015; 3(2):45-50. doi: 10.12691/ajcp-3-2-4

Abstract

Objective: Juglans regia (walnuts), the royal species from Junglandaceae family, well-known for its valuable medicinal uses, their regular consumption may have beneficial effects against oxidative stress mediated diseases including cancer. The present study was aimed to explore the total phenolic content, anti-proliferative and anti-oxidant activity of Juglans regia leaves. Methods: The leaf powder was extracted using different solvents and subjected for phytochemical investigation. The total phenolic contents were determined by the Folin–Ciocalteu method. The extracts comprising a good amount of secondary metabolites especially polyphenols were used to evaluate their antioxidant activity using Fenton’s reagent reaction and DPPH scavenging assay, while cytotoxic and anti-proliferative activity against B16F10 mice melanoma and A375 human melanoma cell were screened using MTT Assay. Observation: Methanolic extract presented the highest total phenolic content (94.39 ± 5.63 mg of GAE/g of extract) as compared to aqueous extract (27.92 ± 1.40 mg of GAE/g of extract). Similarly, methanolic extract presented the highest antioxidant activity (EC50 of 0.250mg/ml) followed by water extract (EC50 of 0.325mg/ml) in Fenton’s reaction and 0.199 ± 0.023 and 2.991 ± 0.740, respectively in DPPH assay. The extracts showed concentration dependent growth inhibition activity (IC50 0.234 and 0.304mg/ml) against B16F10 mice melanoma and A375 human melanoma cell line (IC50 0.298 and 0.350mg/ml) respectively. The extracts proved least toxic when treated with normal lymphocytes. The results indicate that walnut leaves are an excellent source of antioxidant and anti-cancerous agents and may prove fruitful herbal remedy in near future. However, the extracts proved effective against mice melanoma and human melanoma cells. Despite, more study is required before coming to any conclusion.

Keywords:
J. regia anti-oxidant anti-proliferative B16F10 A375 Lymphocytes MTT

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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References:

[1]  Block, G., Patterson, B., Subar, A., 1992. Fruit, vegetables, and cancer prevention: a review of the epidemiological evidence. Nutr. Cancer 18, 1-29.
 
[2]  Heimendinger, J., Van Duyn, M.A., Chapelsky, D., Foerster, S., Stables, G., 1996. The national 5 A Day for Better Health Program: a large-scale nutrition intervention. J. Public Health Manag. Pract. 2, 27-35.
 
[3]  Reddy, L., Odhav, B., Bhoola, K.D., 2003. Natural products for cancer prevention: a global perspective. Pharmacol. Ther. 99, 1-13.
 
[4]  Mathew, A., Peters, U., Chatterjee, N., Kulldorff, M., Sinha, R., 2004. Fat, fiber, fruits, vegetables, and risk of colorectal adenomas. Int. J. Cancer 108, 287-292.
 
[5]  Jenab, M., Ferrari, P., Slimani, N., et al., 2004. Association of nut and seed intake with colorectal cancer risk in the European Prospective investigation into cancer and nutrition. Cancer Epidemiol. Biomarkers Prev. 13, 1595-1603.
 
[6]  Cao, G., Sofic, E., Prior, R., 1996. Antioxidant capacity of tea and common vegetables. J. Agr. Food Chem. 44, 3426-3431.
 
[7]  Silva, B.M., Andrade, P.B., Valentão, P., Ferreres, F., Seabra, R.M., Ferreira, M.A., 2004. Quince (Cydonia oblonga Miller) seed (pulp, peel, and seed) and jam: antioxidant activity. J. Agr. Food Chem. 52, 4405-4712.
 
[8]  Silva, B.M., Valentão, P., Seabra, R.M., Andrade, P.B., 2008. Quince (Cydonia oblonga Miller): an interesting dietary source of bioactive compounds. In: Papadopoulos, K.N. (Ed.), Food Chemistry Research Developments. Nova Science Publishers, Inc., New York, pp. 243-266.
 
[9]  Giada, M.L., Filho, J.M., 2006. The importance of dietary phenolic compounds in the promotion of human health. Biol. Health Sci. 12, 7-15.
 
[10]  Sun, J., Chu, Y.F., Wu, X., Liu, R., 2002. Antioxidant and antiproliferative activities of common seeds. J. Agr. Food Chem. 50, 7449-7454.
 
[11]  Parry, J., SU, L., Moore, J., Cheng, Z., Lutner, M., Rao, J.N., Wang, J. Y., YU, L., 2006. Chemical compositions, antioxidant capacities, and antiproliferative activities of selected seed flours. J. Agr. Food Chem. 54, 3773-3778.
 
[12]  Yang, J., Liu, R., Halim, L., 2009. Antioxidant and antiproliferative activities of common edible nut seeds. Food Sci. Technol. 42, 1-8.
 
[13]  Blomhoff, R., Carlsen, M.,Andersen, L., Jacobs Jr., D., 2006. Health benefits of nuts: potential role of antioxidants. Br. J. Nutr. 96, S52–S60.
 
[14]  Oliveira, I., Sousa, A., Ferreira, I., Bento, A., Estevinho, L., Pereira, J.A., 2008. Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food Chem. Toxicol. 46, 2326-2331.
 
[15]  Bruneton, J., 1999. Pharmacognosie, phytochimie, plantes médicinales. In: Technique et Documentation Lavoisier, Paris, pp. 418-419.
 
[16]  Amaral, J., Seabra, R.M., Andrade, P.B., Valentão, P., Pereira, J.A., Ferreres, F., 2004.Phenolic profile in the quality control of walnut (Juglans regia L.) leaves. Food Chem. 88, 373-379.
 
[17]  Pereira, J.A., Oliveira, I., Sousa, A., Valentão, P., Andrade, P.B., Ferreira, I., Ferreres, F., Bento, A., Seabra, R.M., Estevinho, L., 2007. Walnut (Juglans regia L.) leaves: phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. Food Chem. Toxicol. 45, 2287-2295.
 
[18]  Pereira, J.A., Oliveira, I., Sousa, A., Ferreira, I., Bento, A., Estevinho, L., 2008. Bioactive properties and chemical composition of six walnut (Juglans regia L.) cultivars. Food Chem. Toxicol. 46, 2103-2111.
 
[19]  Singleton, V.L., Rossi, J.A.Jr., 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult. 16, 144-158.
 
[20]  Shah, T.I., Akthar, S., Ganesh, N., 2013. Prelimnary phytochemical evaluation and anti-bacterial potential of different leaf extracts of J. regia: A ubiquitous dry fruit from Kashmir-India. Pharm. Sci. Rev. Res., 19(2), Mar-Apr 2013; n0 18, 93-96.
 
[21]  Almeida, I., Fernandes, E., Lima, J., Costa, P., Bahia, M.F., 2008. Walnut (Juglans regia) leaf extracts are strong scavengers of pro-oxidant reactive species. Food Chem. 106, 1014-1020.
 
[22]  Hatano, T., Kagawa, H., Yasuhara, T., Okuda, T., 1988. Two new flavonoids and other constituents in licorice root: their relative astringency and scavenging effects. Chem. Pharm. Bull. 36, 2090-2097.
 
[23]  Chen, C.Y., Blumberg, J.B., 2008. Phytochemical composition of nuts. Asia Pac. J. Clin. Nutr. 17, 329-332.
 
[24]  Fukuda, T., Ito, H., Yoshida, T., 2003. Antioxidative polyphenols from walnuts (Juglans regia L.). Phytochemistry 63, 795-801.
 
[25]  Khandelwal KR, 2005. Practical Pharmacognocy 16th ed. Pune: Nirali Prakashan.
 
[26]  Umadevi P., A. Ganasoundari, B.S. Rao and K.K.Srinivasan. 1999. In vivo radioprotection by Ocimum flavanoids: survival of mice. Radiat. Res, 151 (1), 74-78.
 
[27]  Kaur, K., Michael, H., Arora, S., Härkönen, P.L., Kumar, S., 2003. Studies on correlation of antimutagenic and antiproliferative activities of Juglans regia L.. J. Environ. Pathol. Toxicol. Oncol. 22, 59-67.
 
[28]  Fleshner, N.E., 2002. Vitamin E and prostate cancer. Urol. Clin. North Am. 29, 107-113.
 
[29]  Spaccarotella, K.J., Kris-Etherton, P.M., Stone, W.L., Bagshaw, D.M., Fishell, V.K., West, S.G., Lawrence, F.R., Hartman, T.J., 2008. The effect of walnut intake on factors related to prostate and vascular health in older men. Nutr. J. 2, 7-13.
 
[30]  Bradford, P.G., Awad, A.B., 2007. Phytosterols as anticancer compounds. Mol. Nutr. Food Res. 51, 161-170.
 
[31]  Han, D.H., Lee, M.J., Kim, J.H., 2006. Antioxidant and apoptosis-inducing activities of ellagic acid. Anticancer Res. 26, 3601–3606.
 
[32]  Losso, J.N., Bansode, R.R., Trappey, A., Bawadi, H.A., Truax, R., 2004. In vitro anti- proliferative activities of ellagic acid. J. Nutr. Biochem. 15, 672-678.
 
[33]  Yáñez, J., Vicente, V., Alcaraz, M., Catillo, J., Benavente-García, O., Canteras, M., Teruel, J., 2004. Cytotoxicity and antiproliferative activities of several phenolic compounds against three melanocytes cell lines: relationship between structure and activity. Nutr. Cancer 49, 191-199.
 
[34]  Kawaii, S., Tomono, Y., Katase, E., Ogawa, K., Yano, M., 1999. Antiproliferative activity of flavonoids on several cancer cell lines. Biosci. Biotechnol. Biochem. 63, 896-899.
 
[35]  Bhuvaneswari, V., Nagini, S., 2005. Lycopene: a review of its potential as an anticancer agent. Curr. Med. Chem. Anticancer Agents 5, 627-635.
 
[36]  Srinivasan, V., Spence, D.W., Pandi-Perumal, S.R., Trakht, I., Cardinali, D.P., 2008. Therapeutic actions of melatonin in cancer: possible mechanisms. Integr. Cancer Ther. 7, 189-203.
 
[37]  Fiorentino, A., D’Abrosca, B., Pacifico, S., Mastellone, C., Piscopo, V., Caputo, R., Monaco, P., 2008. Isolation and structure elucidation of antioxidant polyphenols from quince (Cydonia vulgaris) peels. J. Agr. Food Chem. 56, 2660-2667.
 
[38]  Zhang, Y., Seeram, N.P., Lee, R., Feng, L., Heber, D., 2008. Isolation and identification of straw berry phenolics with antioxidant and human cancer cell antiproliferative properties. J. Agr. Food Chem. 56, 670-675.
 
[39]  Mertens-Talcott, S.U., Lee, H., Percival, S.S., Talcott, S.T., 2006. Induction of cell death in Caco-2 human colon carcinoma cells by ellagic acid rich fractions from muscadine grapes (Vitis rotundifolia). J. Agr. Food Chem. 54, 5336-5343.
 
[40]  Russo, G.L., 2007. Ins and outs of dietary phytochemicals in cancer chemoprevention. Biochem. Pharmacol. 74, 533-544.
 
[41]  Bonfili, L., Cecarini, V., Amici, M., Cuccioloni, M., Angeletti, M., Keller, J.N., Eleuteri, A.M., 2008. Natural polyphenols as proteasome modulators and their role as anticancer compounds. FEBS J. 275, 5512-5526.
 
[42]  Fresco, P., Borges, F., Diniz, C., Marques, M.P., 2006. New insights on the anticancer properties of dietary polyphenols. Med. Res. Rev. 26, 747-766.
 
[43]  Liu, R.H., 2003. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am. J. Clin. Nutr. 78, 517-520.