American Journal of Cancer Prevention
ISSN (Print): 2328-7314 ISSN (Online): 2328-7322 Website: http://www.sciepub.com/journal/ajcp Editor-in-chief: Nabil Abdel-Hamid
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American Journal of Cancer Prevention. 2019, 7(1), 1-9
DOI: 10.12691/ajcp-7-1-1
Open AccessArticle

Benzoin Thiosemicarbazone Inhibits Growth and Triggers Apoptosis in Earlich Ascites Carcinoma Cells through an Intrinsic Pathway

Hossain Mohammad Zakir1, , Md. Mahmudul Hassan Mondol2, , Sha Md. Shahan Shahriar3, Md. Amran Hossain1, Md Abdur Rahim2, Amit Kumar Dey2, Ruhul Amin2, Md. Zamilur Rahman2, Arfatun Nahar Chowdhury2, Md. Moniruzzaman2, Jahanara Khanam4, Md Murshed Hasan Sarkar2, Shaikh M Mohsin Ali3 and Mele Jesmin3

1Department of Chemical Engineering, Z. H. Sikder University of Science and Technology, Sharaitpur, Bangladesh

2Bangladesh Council of Scientific and Industrial Research Laboratories, Rajshahi, Bangladesh

3Department of Applied Chemistry and Chemical Engineering, University of Rajshahi, Rajshahi-6205, Bangladesh

4Department of Biochemistry & Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh

Pub. Date: July 22, 2019

Cite this paper:
Hossain Mohammad Zakir, Md. Mahmudul Hassan Mondol, Sha Md. Shahan Shahriar, Md. Amran Hossain, Md Abdur Rahim, Amit Kumar Dey, Ruhul Amin, Md. Zamilur Rahman, Arfatun Nahar Chowdhury, Md. Moniruzzaman, Jahanara Khanam, Md Murshed Hasan Sarkar, Shaikh M Mohsin Ali and Mele Jesmin. Benzoin Thiosemicarbazone Inhibits Growth and Triggers Apoptosis in Earlich Ascites Carcinoma Cells through an Intrinsic Pathway. American Journal of Cancer Prevention. 2019; 7(1):1-9. doi: 10.12691/ajcp-7-1-1

Abstract

Anticancer drug development is now an emerging field of research all over the world. In this study we attempt to synthesize a novel, simple, inexpensive and safe chemical agents Benzoin thiosemicarbazone (BTSC) and studied against Ehrlich Ascites Carcinoma (EAC) cells bearing Swiss Albino mice by monitoring in vivo tumor cell growth inhibition, survival time of tumor bearing swiss albino mice. MTT colorimetric assay was done to assess the in vitro effect of the test compound. The intrinsic apoptotic pathway induced by BTSC was evidenced by p53 or tumor protein, B-cell lymphoma 2 (BCL-2), B-cell lymphoma extra-large (BCL-xL), BCL-2 associated X protein (BAX), cleavage of caspase-9 and caspase-3 and poly-ADP ribose polymerase (PARP-1). Reactive oxygen species (ROS) generation after BTSC treatment was determined by 2´, 7´- dicholorodihydrofluorescein diacetate (DCFH-DA) staining. The compound was found to possess pronounced anticancer effect. Maximum cell growth inhibition, enhancement of life span was found 73.53% and 52.17% at the dose of 8 mg/kg (i.p) respectively. The induction of apoptosis by BTSC occurred through an ROS-dependent mitochondria-mediated intrinsic pathway rather than an extrinsic pathway, and was regulated by the BCL-2 protein family. The compound exerted a potent antitumor effect toward Ehrlich ascites carcinoma cells through the induction of apoptosis via an intrinsic pathway. Thus, this study provides evidence to carry out further researches in a way to formulate novel anticancer drugs.

Keywords:
EAC cells benzoin thiosemicarbazone intrinsic pathway ROS caspase inhibitor

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