American Journal of Clinical Medicine Research
ISSN (Print): 2328-4005 ISSN (Online): 2328-403X Website: http://www.sciepub.com/journal/ajcmr Editor-in-chief: Dario Galante
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American Journal of Clinical Medicine Research. 2018, 6(3), 58-64
DOI: 10.12691/ajcmr-6-3-2
Open AccessArticle

Comparing Urinary Effect Size Related to Behavioral Symptoms between Total Antioxidant Capacity and Hexanoyl-lysine in Individuals with Autism Spectrum Disorders

Kunio Yui1, , Hitomi Sasaki1, Ryoichi Shiroki1 and Yohei Kawasaki2

1Department of Urology, Fujita health University Graduate School of Medicine, Toyoake, 470-1192, Aichi, Japan

2The Chiba University Clinical Research Center, Chiba, Japan

Pub. Date: September 28, 2018

Cite this paper:
Kunio Yui, Hitomi Sasaki, Ryoichi Shiroki and Yohei Kawasaki. Comparing Urinary Effect Size Related to Behavioral Symptoms between Total Antioxidant Capacity and Hexanoyl-lysine in Individuals with Autism Spectrum Disorders. American Journal of Clinical Medicine Research. 2018; 6(3):58-64. doi: 10.12691/ajcmr-6-3-2

Abstract

The imbalance between oxidative stress such as increased free radicals and decreased antioxidant capacity has been implicated in the etiology of autism spectrum disorders (ASD). However. which of these has a greater effects on ASD behavioral symptoms is still unclear. We measured urinary levels of the oxidative stress biomarker hexanoyl-lysine (HEL) and the total antioxidant capacity (TAC) and the plasma levels of the oxidative stress biomarker superoxide dismutase (SOD) and of the anti-inflammatory fatty acid eicosapentaenoic acid (EPA). We examined the relationships between these biomarkers and behavioral symptoms in 19 individuals with ASD (mean age 10.9 ± 5.3 years) and 11 healthy controls (mean age 14.3 ± 6.3 years). Ages were not no significant difference between the two groups. Behavioral symptoms were assessed using the Aberrant Behavior Checklist (ABC). In the ASD group, urinary TAC levels were significantly lower and urinary HEL levels were significantly higher than in the control group without plasma SOD and EPA levels. ABC scores were significantly higher in the ASD group than in the control group. Stepwise regression analysis and the standardized regression coefficient revealed that urinary TAC levels provided greater impact for distinguishing the two groups, and that that TAOC levels had a larger effect size than HEL levels in urine. Plasma SOD levels were significantly correlated with the ABC irritability and stereotypy score. Thus, urinary TAP levels may be important factor in the pathophysiology of ASD, and altered plasma SOD levels may contribute to the autistic behaviors.

Keywords:
urinary effect size behavioral symptoms total antioxidant power hexanoyl-lysine superoxide dismutase eicosapentaenoic acid autism spectrum disorder

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References:

[1]  Karimi P, Kamali E, Mousavi SM, Karahmadi M. Environmental factors influencing the risk of autism. J Res Med Sci. 2017; 22:27.
 
[2]  Ozonoff S, Heung K, Byrd R, Hansen R, Hertz-Picciotto I. The onset of autism: patterns of symptom emergence in the first years of life. Autism Res. 2008; 1: 320-28.
 
[3]  James SJ, Melnyk S, Jernigan S, Cleves MA, Halsted CH, et al. 2006. Metabolic endophenotype and related genotypes are associated with oxidative stress in children with autism. Am J Med Genet. B Neuropsychiatr. Genet. 2006; 141B: 947-56.
 
[4]  Alabdali A, Al-Ayadhi L, El-Ansary A. 2024. A key role for an impaired detoxification mechanism in the etiology and severity of autism spectrum disorders. Behav. Brain Funct. 2014; 28: 10: 14.
 
[5]  Ghezzo A, Visconti P, Abruzzo PM, Bolotta A, Ferreri C, Gobbi G, et al. erythrocyte membrane alterations in children with autism: correlation with clinical features. PLoS One. 2013; 8: e66418.
 
[6]  Melnyk S, Fuchs GJ, Schulz E, Lopez M, Kahler SG, Fussell JJ, et al. Mtabolic imbalance associated with methylation dysregulation and oxidative damage in children with autism. J Autism Dev Disord. 2010; 42: 367-77.
 
[7]  Ranjbar A, Rashedi V, Rezaei M. Comparison of urinary oxidative biomarkers in Iranian children with autism. Dev Disabil. 2014; 35: 2751-5.
 
[8]  Damodaran LP, Arumugam G. Urinary oxidative stress markers in children with autism. Redox Rep. 2011;16: 216-22.
 
[9]  Tokuda F, Y. Sando Y, H. Matsui H, Yokoyama T. N epsilon-(hexanoyl) lysine, a new oxidative stress marker, is increased in metabolic syndrome, but not in obstructive sleep apnea. Am J Med Sci. 2009; 338: 127-33.
 
[10]  Miyata R, Tanuma N, Sakuma H, Hayashi H. Circadian rhythms oxative stress markers and melatonin metabolite in patients with xeroderma Ppgmentosum goup A. Oxid Med Cell Longev. 2016; 5741517.
 
[11]  Wang L, Jia J, Zhang J, Li K. 2016. Serum levels of SOD and risk of autism spectrum disorder: A case-control study. Int J Dev Neurosci. 2016; 51: 12-6.
 
[12]  Kondolot M, Ozmert EN, Ascı A, Erkekoglu P, Oztop DB, Gumus H, et al. Plasma phthalate and bisphenol a levels and oxidant-antioxidant status in autistic children. Environ Toxicol Pharmacol. 2016; 43, 149-58.
 
[13]  Yorbik O, Sayal A, Akay C, Akbiyik DI, Sohmen T. 2002. Investigation of antioxidant enzymes in children with autistic disorder. Prostaglands. Leukot. Essent. Fatty Acids. 2002; 67: 341-3.
 
[14]  Sögüt S, Zoroğlu SS, Özyurt H, Yilmaz HR, Ozuğurlu F, Sivasli E, et al. Changes in nitric oxide levels and antioxidant enzyme activities may hay a role in the pathophysiolgical mechanisms involved in autism. Clin Chim Acta 2003; 331, 111-7.
 
[15]  Ghanizadeh A, Akhondzadeh S, Hormozi M, Makarem A, Abotorabi-Zarchi M, Firoozabadi A. 2012. Glutathione-related factors and oxidative stress in autism, a review. Curr Med Chem. 2012; 19: 4000-5.
 
[16]  Zoroglu SS, Armutcu F, Ozen S, Gurel A, Sivasli EO, YetkinMeram L. Increased oxidative stress and altered activities of erythrocyte free radical scavenging enzymes in autism.Eur. Arch Psychiatry Clin Neurosci. 2004; 254: 143-7.
 
[17]  Al-Gadani A, El-Ansary A, Attas O, Al-Ayadhi L. Metabolic biomarkers related to oxidative stress and antioxidant status in Saudi autistic children. Clin. Biochem. 2009; 42: 1032-140.
 
[18]  Anderson EJ, Thayne KA, Harris M, Shaikh SR, Darden TM, Lark DS, et al., 2014. Do fish oil omega-3 fatty acids enhance antioxidant capacity and mitochondrial fatty acid oxidation in human atrial myocardium via PPARγ activation? Antioxid Redox Signal. 2014; 21: 1156-63.
 
[19]  Morin C, Blier PU, Fortin S. Eicosapentaenoic acid and docosapentaenoic acid monoglycerides are more potent than docosahexaenoic acid monoglyceride to resolve inflammation in a rheumatoid arthritis model. Arthritis Res Ther. 2015; 17: 142.
 
[20]  Yui K, Tanuma N, Yamada H, Kawasaki Y. Reduced endogenous urinary total antioxidant power and its relation of plasma antioxidant activity of superoxide dismutase in individuals with autism spectrum disorder. Int J Dev Neurosci. 2016; 60: 70-7.
 
[21]  Yui K, Tanuma N, Imataka G, Kawasaki Y. Imbalance between urinary hexanoyl-lysine and total antioxidant capacity levels and plasma superoxide dismutase levels in individuals with autism spectrum disorder. 2017; 3: 1-10.
 
[22]  Yui K, Tanuma N, Yamada H, Kawasaki Y. Decreased total antioxidant capacity has a larger effect size than increased oxidant levels in urine in individuals with autism spectrum disorder. Environ Sci Pollut Res Int. 2017;24: 9635-44
 
[23]  Kim RS. Standardised regression coefficients as indices of effect sizes in meta-analysis. 2011. Florida State University libraries, The Florida State University College of Education, Florida.
 
[24]  American Psychiatric Association. 2013. Diagnostic and statistical manual of mental disorders. 5th ed. American Psychiatric association, Washington, DC.
 
[25]  Rutter M, Le Couteur A, Lord C. ADI-R Autism Diagnostic Interview Revised. 2003; Manual. Western Psychological Services, Los Angeles.
 
[26]  Whehsler D (1974) Wechsler Intelligence Scale for Children-Revised Manual. The Psychological Corporation, New York.
 
[27]  Wechsler D (1981) Wechsler Intelligence Scale for Children-Revised Manual. The Psychological Corporation: San Antonio, TX.
 
[28]  Koyama T, Kamio Y, Inada N, Kurita H. Sex differences in WISC-III profiles of children with high-functioning pervasive developmental disorders J. Autism. Dev. Disord. 2009; 39: 135-41.
 
[29]  Matsui Y, Satoh K, Miyazaki T, Shirabe S, Atarashi R, Mutsukura K, et al. High sensitivity of an ELISA kit for detection of the gamma-isoform of 14-3-3 proteins: usefulness in laboratory diagnosis of human prion disease. BMC Neurol. 2011; 11: 120.
 
[30]  Boonla C, Wunsuwan R, Tungsanga K, Tosukhowong P, Urinary 8-hydroxydeoxyguanosine is elevated in patients with nephrolithiasis. Urol Res. 2007; 35: 185-91.
 
[31]  Rojahn J, Aman MG, Matson JL, Mayville E. The Aberrant Behavior Checklist and the Behavior Problems Inventory: convergent and divergent validity. Res. Dev. Disabil. 2003; 24: 391-404.
 
[32]  Hollander E, Chaplin W, Soorya L, Wasserman S, Novotny S, Rusoff J. Divalproex sodium vs placebo for the treatment of irritability in children and adolescents with autism spectrum disorders. Neuropsychopharmacology. 2010; 35: 990-8.
 
[33]  Karabekiroglu K, Aman MG. Validity of the aberrant behavior checklist in a clinical sample of toddlers. Child Psychiatry Hum Dev. 2009; 40: 99-110.
 
[34]  Singh K, Connors SL, Macklin EA, Smith KD, Fahey JW, Talalay P. Sulforaphane treatment of autism spectrum disorder (ASD). Proc Natl Acad Sci U S A. 2014; 111: 15550-5.
 
[35]  Balcerczyk A, Bartosz G. Thiols are main determinants of total antioxidant capacity of cellular homogenates. Free Radic Res. 2003; 37: 537-41.
 
[36]  Ming X, Stein TP, Brimacombe M, Johnson WG, Lambert GH, Wagner GC. Increased excretion of a lipid peroxidation biomarker in autism. Prostaglandins Leukot. Essent. Fatty Acids. 2005; 73: 379-84.
 
[37]  James SJ, Melnyk S, Jernigan S, Cleves MA, Halsted CH, Wong DH, et al, Metabolic endophenotype and related genotypes are associated with oxidative stress in children with autism. Am J Med Genet B Neuropsychiatr Genet. 2006; 141B: 947-56.
 
[38]  Nardone S, Sams DS, Reuveni E, Getselter D, Oron O, Karpuj M, et al. DNA methylation analysis of the autistic brain reveals multiple dysregulated biological pathways. Transl Psychiatry. 2014; 4, e433.
 
[39]  Ayadhi AL, Elamin NE. Camel Milk as a Potential Therapy as an Antioxidant in Autism Spectrum Disorder (ASD). Evid Based Complement Alternat Med. 2013; 602834.
 
[40]  Esposito L, Raber J, Kekonius L, Yan F, Yu GQ, Bien-L, N, et al. Reduction in mitochondrial superoxide dismutase modulates Alzheimer's disease-like pathology and accelerates the onset of behavioral changes in human amyloid precursor protein transgenic mice. J. Neurosci. 2006; 26: 5167-79.
 
[41]  Sagduyu K, Dokucu ME, Eddy BA, Craigen G, Baldassano CF, Yildiz A. Omega-3 fatty acids decreased irritability of patients with bipolar disorder in an add-on, open label study. Nutr J. 2005; 4: 6.
 
[42]  Lotrich FE, Sears B, McNamara RK. Anger induced by interferon-alpha is moderated by ratio of arachidonic acid to omega-3 fatty acids. J Psychosom Res 2013; 75: 475-83.
 
[43]  Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe SE. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014; 94: 329-54.
 
[44]  Miglio C, Peluso I, Raguzzini A, Villaño DV, Cesqui E, Catasta G, et al. Fruit juice drinks prevent endogenous antioxidant response to high-fat meal ingestion. Br J Nutr. 2014; 111, 294-300.
 
[45]  Papadia S, Soriano FX, Léveillé F, Martel MA, Dakin KA, Hansen HH, et al. Synaptic NMDA receptor activity boosts intrinsic antioxidant defenses. Nat Neurosci. 2008; 11: 476-87.
 
[46]  Liu S, Li X, Wu S, He J, Pang C, Deng Y, Dong R. Fungal pretreatment by phanerochaete chrysosporium for enhancement of biogas production from corn stover silage. Appl Biochem Biotechnol. 2014; 174: 1907-18.
 
[47]  Matayatsuk C, Lee CY, Kalpravidh RW, Sirankapracha P, Wilairat P, Fucharoen S, et al. 2007. Elevated F2-isoprostanes in thalassemic patients. Free Radic Biol Med. 2007; 43: 1649-55.
 
[48]  Baron-Cohen S, Lombardo MV, Auyeung B, Ashwin E, Chakrabarti B, Knickmeyer R. Why are autism spectrum conditions more prevalent in males? PLoS Biol. 2012; 9; e1001081.