American Journal of Clinical Medicine Research
ISSN (Print): 2328-4005 ISSN (Online): 2328-403X Website: http://www.sciepub.com/journal/ajcmr Editor-in-chief: Dario Galante
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American Journal of Clinical Medicine Research. 2017, 5(4), 55-58
DOI: 10.12691/ajcmr-5-4-4
Open AccessArticle

Impact of Reactive Dengue NS1 Antigen with Time for Hospital Admission in Dengue Viral Infection Patient

Riyadi Adrizain1, and Djatnika Setiabudi1

1Department of Child Health, Padjadjaran University/Hasan Sadikin General Hospital, Bandung, Indonesia

Pub. Date: July 18, 2017

Cite this paper:
Riyadi Adrizain and Djatnika Setiabudi. Impact of Reactive Dengue NS1 Antigen with Time for Hospital Admission in Dengue Viral Infection Patient. American Journal of Clinical Medicine Research. 2017; 5(4):55-58. doi: 10.12691/ajcmr-5-4-4

Abstract

Dengue is the most rapidly spreading mosquito-borne viral disease worldwide with estimated 30-fold increase in incidence over the last five decades. The recent study had found an increase in the number of admissions of cases of dengue fever with reactive NS1 antigen. The aim of this study was to evaluate the utility of NS1 antigen as a diagnostic marker and to determine whether there is a higher risk of severe dengue infection for patient with positive NS1 antigen. This was a retrospective analysis study conducted in 7 referral Hospitals in Bandung, West Java, Indonesia. The medical records of all the individuals admitted to the Hospital from 1 January to 31 December 2015 were evaluated. We used a questionnaire as a tool for data collection consisted of the epidemiological, clinical manifestation, laboratory results and final diagnosis data that were collected during the period of hospitalizing. In 2015 there were 4.096 cases of dengue viral infection hospitalized. Most of the patients with positive NS1 antigen were admitted at early phase (0-2 days) and regular phase (3-5 days). Platelet count >100.000/mm3 was significantly associated with positive NS1Ag assay, while lower thrombocytopenia (platelet count <50.000/mm3) was significantly associated with positive IgM dengue antibody. Severe cases of dengue viral infection of DHF significantly more often happen on patient with IgM antibody dengue positive (P<0.005). NS1 Ag assay is useful, sensitive and specific for the diagnosis of dengue detection, with good sensitivity and specificity during acute phase when detectable antibodies still negative.

Keywords:
dengue NS1 hospital admission surveillance

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References:

[1]  Pothapregada, S., Kamalakannan, B., Thulasingam, M., and Sampath, S., “Is Reactive Dengue NS1Antigen Test a Warning Call for Hospital Admissions?,” Journal of Clinical and Diagnostic Research, 10 (4). 4-7. Apr.2016.
 
[2]  Ahmed, N.H., and Broor, S., “Comparison of NS1 antigen detection ELISA, real time RT-PCR and virus isolation for rapid diagnosis of dengue infection in acute phase,” Journal of Vector Borne Disease, 51. 194-199. Sep.2014.
 
[3]  Peeling, R.W., et al., “Evaluation of diagnostic tests: dengue,” Nature Reviews, s30-s38. Dec.2010.
 
[4]  Schilling, S., Ludolfs, D., Van An, L., and Schmitz, H., “Laboratory diagnosis of primary and secondary dengue infection,” Journal of Clinical Virology, 31 (3). 179-184. 2004.
 
[5]  Aryati, A., et al., “Performance of commercial dengue NS1 ELISA and molecular analysis of NS1 gene of dengue viruses obtained during surveillance in Indonesia,” BMC Infectious Diseases, 13 (611). 2013.
 
[6]  Simmons, C.P., Farrar, J. J., Van VC N., and Wills, B., “Dengue,” New England Journal of Medicine, 366. 1423-1432. 2012.
 
[7]  World Health Organization, Dengue hemorrhagic fever: diagnosis, treatment, prevention and control, World Health Organization, Geneva, 1997.
 
[8]  World Health Organization-SEARO, Comprehensive guidelines for prevention and control of Dengue and Dengue Haemorrhagic Fever, World Health Organization-SEARO, New Delhi, 2011.
 
[9]  Datta, W., and Wattal, C., “Dengue NS1 antigen detection test: A useful tool in early diagnosis of dengue virus infection,” Indian Journal of Medical Microbiology 28. 107-110. 2006.
 
[10]  Henchal, E. A., McCown, J. M., Sequin, M. C., Gentry M. K., and Brandt, W. E., “Rapid identification of dengue virus isolates by using monoclonal antibodies in an indirect immunofluroescent assay,” American Journal of Tropical Medicine Hygiene, 32. 164-169. 1983.
 
[11]  Abello, J. E., Cuesta, J. G., Cerro, B. R., and Guha-Sapir, D., “Factors Associated with the Time of Admission among Notified Dengue Fever Cases in Region VIII Philippines from 2008 to 2014,” PLOS Neglected Tropical Diseases. Oct.2016.
 
[12]  Chairulfatah, A., Setiabudi, A., Agoes R., vonSprundel, M., and Colebunders, R., “Hospital-based clinical surveillance for dengue heamorhagic fever in Bandung, Indonesia” Acta Tropica, 80. 111-115. 2001.
 
[13]  De Souza, L. J., et al., “Comparison of clinical and laboratory characteristics between children and adults with dengue,” The Brazillian Journal of Infectious Diseases, 17 (1). 27-31. Jan.2013.
 
[14]  Wang, C., et al., “Differences in clinical and laboratory characteristics and disease severity between children and adults with dengue virus infection in Taiwan,” Transactions of the Royal Society of Tropical Medicine and Hygiene, 103. 871-877. 2009.
 
[15]  Libraty D.H., et al., “High Circulating Levels of the Dengue Virus Nonstructural Protein NS1 Early in Dengue Illness Correlate with the Development of Dengue Hemorrhagic Fever,” The Journal of Infectious Diseases, 2002.