American Journal of Clinical Medicine Research
ISSN (Print): 2328-4005 ISSN (Online): 2328-403X Website: http://www.sciepub.com/journal/ajcmr Editor-in-chief: Dario Galante
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American Journal of Clinical Medicine Research. 2015, 3(3), 45-49
DOI: 10.12691/ajcmr-3-3-3
Open AccessArticle

Performance Analysis of Blood Culture by an Automated Blood Culture System at a Tertiary Care Teaching Hospital in South India

K V Ramana1, , Padmawali palange1, Sanjeev D Rao1, Ritu Vaish1 and B Mohan Rao1

1Department of Microbiology, Prathima Institute of Medical Sciences, Karimnagar, Telangana, India

Pub. Date: August 07, 2015

Cite this paper:
K V Ramana, Padmawali palange, Sanjeev D Rao, Ritu Vaish and B Mohan Rao. Performance Analysis of Blood Culture by an Automated Blood Culture System at a Tertiary Care Teaching Hospital in South India. American Journal of Clinical Medicine Research. 2015; 3(3):45-49. doi: 10.12691/ajcmr-3-3-3

Abstract

This Prospective study analyses culture result of 134 blood culture samples received in microbiology laboratory during a five months period between October 2014 and February 2015. We have documented time required for the culture to become positive, time at which culture could be considered negative and the spectrum of isolated organismsms including their antimicrobial susceptibility patterns. The specimens were processed by using automated system BacT/Alert 3D/60. Microorganism’s identification was performed by routine conventional and automated identification system and antibiotic susceptibility testing was done by Kirby bauer disk diffusion method. The mean detection time for isolates was 14.5 +/- 5.7 hours. Among organisms isolated included were Gram positive bacteria, Gram negative bacteria and yeasts with 22 (16.4%), 14 (10.4%) and 19 (14.2%) respectively. All our cultures were positive within 34 hours.

Keywords:
automated culture system BacT/Alert 3D turnaround time blood culture

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References:

[1]  J. M. Loonen, A. R. Jansz, J. Stalpers, P. F. G. Wolffs, A. J. C. van den Brule. An evaluation of three processing methods and the effect of reduced culture times for faster direct identification of pathogens from BacT/ALERT blood cultures by MALDI-TOF MSEur J Clin Microbiol Infect Dis (2012) 31:1575-1583.
 
[2]  Smith, J. A., E. A. Bryce, J. Ngui-Yen, and F. J. Roberts. Comparison of BACTEC 9240 and BacT/Alert blood culture systems in an adult hospital. J. Clin. Microbiol. 1995; 33:1905-1908.
 
[3]  Thorpe, T. C., M. L. Wilson, J. E. Turner, J. L. DiGuiseppi, M. Willert, S. Mirrett, and L. B. Reller. BacT/Alert: an automated colorimetric microbial detection system. J. Clin. Microbiol. 1990; 28:1608-1612.
 
[4]  Wilson, M. L., M. P. Weinstein, S. Mirrett, L. G. Reimer, R. J. Feldman, C. R. Chuard, and L. B. Reller. Controlled evaluation of BacT/Alert standard anaerobic and FAN anaerobic blood culture bottles for the detection of bacteremia and fungemia. J. Clin. Microbiol. 1995; 33:2265-2270.
 
[5]  James H. Jorgensen, Stanley Mirrett, L. C. Mcdonald, Patrick R. Murray, Melvin P. Weinstein, J. Fune, Christa W. Trippy, Marianne Masterson, and L. Barth Reller. Controlled Clinical Laboratory Comparison of BACTEC Plus Aerobic/F Resin Medium with BacT/Alert Aerobic FAN Medium for Detection of Bacteremia and Fungemia. J Clin Microbiol 1997; 35: 53-58.
 
[6]  Rohner P, Pepey B, and Auckenthaler R. Comparison of BacT/Alert with Signal Blood Culture System. J. Clin. Microbiol, Feb. 1995; 33:313-317.
 
[7]  Wilson, M. L., M. P. Weinstein, L. G. Reimer, S. Mirrett, and L. B. Reller. Controlled comparison of the BacT/Alert and BACTEC 660/730 nonradiometric blood culture systems. J. Clin. Microbiol. 1992; 30:323-329.
 
[8]  D J. Hardy, Barbara B. Hulbert, and Paula C. Migneault. Time to Detection of Positive BacT/Alert Blood Cultures and Lack of Need for Routine Subculture of 5- to 7-Day Negative Cultures. J. Clin. Microbiol. 1992; 30:2743-2745
 
[9]  Andries W Dreyer, Nazir A Ismail, Deliwe Nkosi, Kathy Lindeque, Marliza Matthews, Danie G van Zyl, Anwar A Hoosen. Comparison of the VersaTREK blood culture system against the Bactec9240 system in patients with suspected bloodstream infections. Annals of Clinical Microbiology and Antimicrobials 2011,10:4.
 
[10]  Nicasio Mancini, Silvia Carletti, Nadia Ghidoli, Paola Cichero, Roberto Burioni, and Massimo Clementi, The Era of Molecular and Other Non-Culture-Based Methods in Diagnosis of Sepsis. CLINICAL MICROBIOLOGY REVIEWS. 2010, 23: 235-251.
 
[11]  Chukwuemeka II, Samuel Y. Quality assurance in blood culture: A retrospective study of blood culture contamination rate in a tertiary hospital in Nigeria. Niger Med J 2014;55:201-3.
 
[12]  Saleh AA, SattarANI, Ahmed S and Miah MRA. Antibiotic sensitivity pattern of Salmonell species isolated by blood culture in Bangabandhu Sheikh Mujib Medical University, Dhaka. BJMM 2008 Jul; 2(2); 22-36.
 
[13]  ABMA Wadud, Ml Khalil, AKM Shamsuzzaman, Kms Islam, BB Mondal, MZ Banda, MSK Shahid Ullah. Bacteriological profiles of Blood culture isolates by BacT/ALERT 3D automated system Journal of Shaheed Suhrawardy Medical College 2009;1:21-26.
 
[14]  Saha SK, Baqui AH, Hanif M, Darmstadt GL, Ruhulamin M, Nagatake T, et al. Typhoid fever in Bangladesh: implications for vaccination policy. Pediatric Infectious Disease Journal 2001 May; 20(5):521-524.
 
[15]  Shrestha P, Das BK, Bhatta NK, Jha DK, Das B, Setia A, et al. Clinical and Bacteriological Profiles of Blood Culture Positive Sepsis in Newborns. J. Nepal Paediatr.Soc 2008; 27(2): 64-67.
 
[16]  Than Nu Shwe, Mar Mar Nyein, Wut Yi and Aung Mon. Blood culture isolates from children admitted to Medical Unit III, Yangon Children's Hospital. 1998. Southeast Asian J Trap Med Public Health 2002 Dec; 33(4): 764-771.
 
[17]  Ghanshyam D. Kumhar, V.G. Ramachandran and Piyush Gupta. Bacteriological Analysis of Blood Culture Isolates from Neonates in a Tertiary Care Hospital in India. J Health Popul Nutr 2002 Dec: 20(4): 343-347.
 
[18]  Niimi Yoshihiro, Saito Takako and Izumo Taeko. Isolation of microorganisms from blood cultures during 8 years at Sakai Municipal Hospital Frequency of isolation, number of samples per Bacteriological profiles of Blood culture isolates by BacT/ALERT 3D automated system case, and CVC-related bacteremla Sakai Municipal Hospital Medical Journal 2004; 7: 64-71.
 
[19]  Doern GV, Brueggemann AB, Dunne WM, Jenkins SG, Halstead DC.McLaughlin JC. Four-day incubation period for blood culture bottles processed with the Difco ESP blood culture system. J Clin Microbiol 1997; 35:1290-2.
 
[20]  Paul P. Bourbeau and Michael Foltzer. Routine Incubation of BacT/ALERT FA and FN Blood Culture Bottles for More than 3 Days May Not Be Necessary. J Clin Microbiol 2005;43: 2506-2509.
 
[21]  Lynn L. Horvath, Benjamin J. George, Clinton K. Murray, Linda S. Harrison, and Duane R. Hospenthal. Direct Comparison of the BACTEC 9240 and BacT/ALERT 3D Automated Blood Culture Systems for Candida Growth Detection. J Clin Microbiol 2004;42:115-118.