American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: http://www.sciepub.com/journal/ajbr Editor-in-chief: Hari K. Koul
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American Journal of Biomedical Research. 2013, 1(4), 108-111
DOI: 10.12691/ajbr-1-4-6
Open AccessCommentary

A New Overview on the Old Topic: The Theoretical Analysis of “Combinatorial Strategy” for DNA Recombination

Gang Zhang1, 2,

1Department of Cell & Systems Biology, University of Toronto, Toronto, Canada

2Department of Medicine, Centre for Research in Neurodegenerative Diseases, University of Toronto, Toronto, Canada;Division of Nephrology, Massachusetts General Hospital, Harvard Medical School, Harvard University, Charlestown, United States

Pub. Date: November 15, 2013

Cite this paper:
Gang Zhang. A New Overview on the Old Topic: The Theoretical Analysis of “Combinatorial Strategy” for DNA Recombination. American Journal of Biomedical Research. 2013; 1(4):108-111. doi: 10.12691/ajbr-1-4-6

Abstract

To clone genes of interest into suitable vectors is the first step to investigate their functions in vitro and in vivo. At the genome era, the sequences of more and more genes were decoded and available gradually. Therefore, it is critical to develop high efficient strategies for cloning genes of interest into different vectors to facilitate the functional analyses of them. In our previous studies, we created “Combinatorial strategy” for DNA recombination. Here, I theoretically analyzed the procedure of DNA recombination, the mechanism of this strategy, and further gave suggestions and predictions for various ligation-dependent molecular cloning experiments.

Keywords:
combinatorial strategy DNA recombination clone sites CIP T4 DNA ligase transformation Top10

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References:

[1]  Jackson, D.A., Symons, R.H. and Berg, P., Biochemical method for inserting new genetic information into DNA of Simian Virus 40: Circular SV40 DNA molecules containing Lambda phage genes and the galactose operon of Escherichia Coli, Proc Nat Acad Sci USA, 69: 2904-2909, 1972.
 
[2]  Cohen, S.N., Chang, A.C.Y., Boyer, H.W. and Helling, R.B., Construction of biologically functional bacteria plasmids in vitro, Proc Nat Acad Sci USA, 70: 3240-3244, 1973.
 
[3]  Hutchison, C.A., Phillips, S., Edgell, M.H., Gillam, S., Jahnke, P. and Smith, M., Mutagenesis at a specific position in a DNA sequence, J Biol Chem, 253: 6551-6560, 1978.
 
[4]  Adams, M.D., Celniker, S.E., Holt, R.A., et al., The geneome sequence of Drosophila Melanogaster, Science, 287: 2181-2195, 2000.
 
[5]  Mardis, E.R., Next-generation DNA sequencing methods. Annu Rev Genomics Hum Genet, 9: 387-402, 2008.
 
[6]  Mayer, A.N., Dimmock, D.P., Arca, M.J., Bick, D.P., Verbsky, J.W., Worthey, E.A., Jacob, H.J. and Margolis, D.A., A timely arrival for genomic medicine, Genet Med, 13: 195-196, 2011.
 
[7]  Zhang, G. and Tandon, A., Quantitative assessment on the cloning efficiencies of lentiviral transfer vectors with a unique clone site, Sci Rep, 2: 415, 2012.
 
[8]  Zhang, G. and Tandon, A., Combinatorial strategy: A highly efficient method for cloning different vectors with various clone sites, In submission, 2013.
 
[9]  Jung, V., Pestka, S.B. and Pestka, S., Efficient cloning of PCR generated DNA containing terminal restriction endonuclease recognition sites, Nucl Acids Res, 18: 6156, 1990.
 
[10]  Testori, A., Listowsky, I. and Sollitti, P., Direct cloning of unmodified PCR products by exploiting an engineered restriction site, Gene, 143: 151-152, 1994.
 
[11]  Sambrook, J., Fritsch, E.F. and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press: Plainview, NY, 1989.
 
[12]  Norrander, J., Kempe, T. and Messing, J., Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis, Gene, 26: 101-106, 1983.
 
[13]  Lehnman, I.R., DNA ligase: structure, mechanism and function. Science, 186: 790-797, 1974.
 
[14]  Wu, D.Y. and Wallace, R.B., Specificity of the nick-closing activity of bacteriophage T4 DNA ligase, Gene, 76: 245-254, 1989.
 
[15]  Harada, K. and Orgel, L., Unexpected substrate specificity of T4 DNA ligase revealed by in vitro selection, Nucl Acids Res, 21: 2287-2291, 1993.
 
[16]  Landegren, U., Kaiser, R., Sanders, J. and Hood, L., A ligase-mediated gene detection technique, Science, 241: 1077-1080, 1988.
 
[17]  Sambrook, J. and Russell, D.W., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press: Plainview, NY, 2001.