American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: http://www.sciepub.com/journal/ajbr Editor-in-chief: Hari K. Koul
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American Journal of Biomedical Research. 2017, 5(3), 46-56
DOI: 10.12691/ajbr-5-3-2
Open AccessArticle

Dietary Ginger Extracts Enhanced Glucose Uptake by Muscle and Adipose of Normal and Diabetic Rats via Mimicry of Insulin Action

Adeniyi P.O.1, 2, , Sanusi R.A.1 and Obatolu V.A.2

1University of Ibadan, Ibadan, Nigeria

2Institute of Agricultural Research and Training, Ibadan, Obafemi Awolowo University, Ile-Ife, Nigeria

Pub. Date: August 13, 2017

Cite this paper:
Adeniyi P.O., Sanusi R.A. and Obatolu V.A.. Dietary Ginger Extracts Enhanced Glucose Uptake by Muscle and Adipose of Normal and Diabetic Rats via Mimicry of Insulin Action. American Journal of Biomedical Research. 2017; 5(3):46-56. doi: 10.12691/ajbr-5-3-2

Abstract

The mechanism for the blood glucose lowering effect of different ginger extracts is yet to be clearly understood, that of raw ginger extracts has been sparsely explored while the mechanism for the cooked ginger extract, the form in which it is mostly consumed, has not been delved into. This study was therefore designed to determine the effect of raw and cooked ginger (Zingiber officinale Roscoe) extracts on glucose uptake by the muscle and adipose of normal, streptozocin-induced and high-fat diet-induced diabetic rats. Fresh ginger rhizomes were washed, peeled, milled and sieved without adding water to obtain the raw extract. A portion of this was boiled for 1 hour to give the cooked extract. Matured male albino rats (140) were divided into two major groups with 70 rats in each group. Group A was further divided into 7 sub-groups (n=10) 40 of which were rendered diabetic with intraperitoneal injection of streptozocin (60mg/kg body weight) to mimic Type 1 diabetes while in Group B Type 2 diabetes was induced with a 12 week consumption of High-Fat Diet (HFD). The remaining 30 rats in each group were left to remain non-diabetic/ normal. Ginger extracts were administered as a daily oral dose for 4 weeks after diabetes induction. The animals were the sacrificed and glucose uptake by the muscle and adipose tissue was determined using standard analytical procedure. Mean data were compared using Least Significant Difference at p¡Ü 0.05. It was clearly evidenced that both raw and cooked ginger extracts significantly enhanced and increased glucose uptake by the muscle and adipose tissues even in the absence of insulin, hence, mimicking insulin action. Ginger in both raw and cooked forms may therefore be beneficial in the prevention and management of diabetes mellitus. Human trial is hereby recommended.

Keywords:
raw ginger extract cooked ginger extract glucose uptake diabetes mellitus

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References:

[1]  Mozaffari-Khosravi H., Talaei B., Jalali B-A., Najarzadeh A. and Mozayan M.R. (2014). The effect of ginger powder supplementation on insulin resistance and glycemic indices in patients with Type 2 diabetes: A randomized double-blind placebo-controlled trial. Complementary Therapies in Medicine; 22(1): 9-16.
 
[2]  Mahluji S., Attari V.E., Mabassori M., Payahoo L., Ostadrahimi A. and Golzari S.E.J. (2013). Effect of ginger (Zingiber officinale) on plasma glucose level, HbA1c and insulin sensitivity in type 2 diabetic patients. International Journal of Food Sciences and Nutrition; 64(6): 682-686.
 
[3]  Son M.J., Miura Y. and Kazum Y. (2014). Mechanism of anti diabetic effect of gingerol in cultured cells and obese diabetic model mice. Cytotechnology.
 
[4]  Sukalingam K., Ganesan K. and Gani S.B. (2013). Hypoglycemic effect of 6-gingerol, an active principle of ginger in streptozotocin-induced diabetic rats. Journal of Pharmacology and Toxicological Studies; 1(2): 23-30.
 
[5]  Abdulrazaq N.B., Cho M.M., Win N.N., Zaman R. and Rahman M.T. (2012). Beneficial effects of ginger (Zingiber officinale) on carbohydrate metabolism in streptozotocin-induced diabetic rats. British Journal of Nutrition; 108(7): 1194-1201.
 
[6]  Jafri S.A., Abass S. and Qasim M. (2011). Hypoglycemic effect of Ginger (Zingiber officinale) in alloxan-induced diabetic rats (Rattus norvagicus). Pakistan Veterinary Journal; 31(2): 160-162.
 
[7]  Anfernan M.L.K. (2014). Evaluation of nutritional and anti diabetic activity of different forms of ginger in rats. Middle East Journal of Scientific Research; 21(1): 56-62.
 
[8]  Akhani S.P., Vishwakarma S.L and Goyal R.K. (2005). Antidiabetic activity of Zingiber officinale Roscoe in streptozotocin-induced non-insulin dependent diabetic rats. Indian Journal of Pharmaceutical Science; 65(5): 553-557.
 
[9]  Kalejaiye O.F., Iwalewa E.O., Omobuwajo O.R. and Oyedapo O.O. (2002). Hypoglycemic effects of Nigerian Zingiber officinale rhizome on experimental diabetic rats. Nigerian Journal of Natural Products and Medicine; 6: 33-35.
 
[10]  Kadnur S.V. and Goyal R.K. (2005). Beneficial effect of Zingiber officinale Roscoe on fructose-induced hyperlipidemia and hyperinsulinemia in rats. Indian Journal of Experimental Biology; 43: 1161-1164.
 
[11]  Islam S. and Choi H. (2008). Comparative effect of dietary ginger (Zingiber officinale) and garlic (Allium sativum) investigated in a type 2 diabetes model of rats. Journal of Medicinal Food; 11(1): 152-159.
 
[12]  Nammi S., Sreemantha S. and Roufogalis B.D. (2009). Protective effects of ethanolic extract of Zingiber officinale rhizome on the development of metabolic syndrome in high-fat diet-fed rats. Basic and Clinical Pharmacology and Toxicology; 104(5): 366-373.
 
[13]  Al-Qattan K., Thomson M. and Ali M. (2008). Garlic (Allium sativum) and ginger (Zingiber officinale) attenuate structural nephropathy progression in streptozotocin-induced diabetic rats. European e-Journal of Clinical Nutrition and Metabolism; 3(2): e62-e71.
 
[14]  Ugwuja E.I., Nwibo A.N., Ugwu N.C. and Aloke C. (2010). Effects of aqueous extract of spices mixture containing curry, garlic and ginger on plasma glucose and lipids in alloxan-induced diabetic rats. Pakistan Journal of Nutrition; 9(12): 1131-1135.
 
[15]  AL-Moramadhi S.A.H. (2010). The effect of Zingiber officinale roots infusion on some physiological parameters in broiler chickens. Kufa Journal of Veterinary MedicalSciences; 1(2) www.uokufa.edu.iq(03/03/2013).
 
[16]  Somani R.S. and Singhai A.K. (2008). Hypoglecemic and hypolipidemic effect of Zingiber officinale in normal and diabetic rats. In Phytopharmacology and Therapeutic Values; eds. Govil J.N. and Singh V.K.; Vol IV: 171-173. Cabdirect, U.K.
 
[17]  Singh A.B., Singh N., Maurya R. and Srivastava A.K. (2009). Anti-hyperglycemic, lipid-lowering and antioxidant properties of [6]-gingerol in db/db mice.. International Journal of Medicine and Medical Sciences; 1(12): 536-544.
 
[18]  Al-Amin Z.M., Thomson M., Al-Qattan K.K., Peltonen-Shalaby R. and Ali M. (2006). Antidiabetic and hypolipidemic properties of ginger (Zingiber officinale) in streptozotocin-induced diabetic rats. British Journal of Nutrition; 96(4): 660-666.
 
[19]  Ojewole J.A.O (2006). Analgesic, Antiinflammatory and Hypoglycemic effects of ethanol extract of Zingiber officinale (Roscoe) rhizomes in mice and rats. Phytotherapy Research: 20: 764-772.
 
[20]  Elshater A.A., Salman M.M.A. and Moussa M.M.A. (2009). Effect of Ginger extract consumption on levels of blood glucose, lipid profile and kidney functions in alloxan-induced diabetic rats. Egyptian Academic Journal of Biological Sciences; 2(1): 153-162.
 
[21]  Akhani S.P., Vishwakarma S.L. and Goyal R.K. (2004). Anti diabetic activity of Zingiber officinale in streptozotocin-induced Type 1 diabetic rats. Journal of Pharmacy and Pharmacology; 56: 101-105.
 
[22]  Sharma M. and Shukla S. (1977). Hypoglycemic effect of ginger.Journal of Research onIndian Yoga and Homeopathy. ; 12: 127-130.
 
[23]  Satiel A.R and Kahn C.R. (2001). Insulin signaling and the regulation of glucose and lipid metabolism. Nature; 404: 799-806.
 
[24]  Imamura T., Huang J., Usui I., Satoh H., Bever J. and Olefsky J.M. (2003). Insulin-induced GLUT4 translocation involves protein kinase C-¦Ë-mediated functional coupling between Rab4 and the motor protein kinesin. Molecular and Cellular Biology; 23(14): 4892-4900.
 
[25]  Huang S. and Czech M.P. (2007). The GLUT4 glucose transporter. Cell metabolism; 5(4): 237-252.
 
[26]  Rani M.P., Krishna M.S., Padmakumari P., Raghu K.G. and Sundaresan A. (2012). Zingiber officinale extract exhibits antidiabetic potential via modulating glucose uptake, protein glycation and inhibiting adipocyte differentiation: an in vitro study. Journal of the Science of Food and Agriculture; 92(9): 1948-1955.
 
[27]  Son M.J., Miura Y. and Kazum Y. (2014). Mechanism of anti diabetic effect of gingerol in cultured cells and obese diabetic model mice. Cytotechnology.
 
[28]  Noipha K. and Ratanachaiyavong S. (2008). Effect of ginger (Zingiber officinale Roscoe) on glucose transport in cultured skeletal muscle cells. Journal of Thai Traditional and Alternative Medicine; 6(2): 82-87.
 
[29]  Martinello F., Soares S.M. and Franco J.J. (2006). Hypolipidemic and antioxidant activates from Tamarindus indica pulp fruit extract in hypercholesterolemic hamsters. Food and Chemical Toxicology; 44(6): 810-818.
 
[30]  Kergoat M. and Portha B. (1985). In vivo hepatic and peripheral insulin sensitivity in rats with non-insulin-dependent diabetes induced by streptozocin. Assessment with the insulin-glucose clamp technique. Diabetes; 34(11): 1120-6.
 
[31]  Gupta R.K., Kesari A.N., Watal G., Murthy P.S., Chandra R., Mathal K and Tandon V. (2005). Hypoglycemic and antidiabetic effect of aqueous extract of leaves of Annona squamosa (L.) in experimental animal. Current Science; 88(8): 1244-1254.
 
[32]  Broadhurst C.L., Polansky M.M.and Anderson R.A. (2000). Insulin-like biological activity of culinary and medicinal plant aqueous extracts in vitro. Journal of Agricultural and Food Chemistry; 48(3): 849-852.
 
[33]  Li Y., Tran V.H., Koolaji N., Duke C. and Roufogalis B.D. (2013). [S]-[6]-gingerol enhances glucose uptake in L6 myotubes by activation of AMPK in response to [Ca2+]. Journal of Pharmacy and Pharmaceutical Sciences; 16(2): 304-312.
 
[34]  Li Y., Tran V.H., Kota B.P., Nammi S., Duke C.C. and Roufogalis B.D. (2014). Preventive effect of Zingiber offinale on insulin resistance in a high-fat high-carbohydrate diet-fed rat model and its mechanism of action. Basic Clinical Pharmacology and Toxicology; 115(2): 209-215.
 
[35]  Sekiya K., Ontani P. and Kusano S. (2004). Enhancement of insulin sensitivity in adipocytes by ginger. Biofactors; 22(1-4): 152-156.
 
[36]  Gannon N.P., Conn C.A., and Vaughan R.A. (2014). Dietary stimulators of GLUT4 expression and translocation in skeletal muscle: A mini-review. Molecular Nutrition and Food Research; 59(1): 48-64.
 
[37]  Adeniyi P.O. and Sanusi R.A. (2014). Effect of ginger (Zingiber officinale) extracts on blood glucose in normal and STZ-induced diabetic rats. International Journal of Clinical Nutrition; 2(2): 32-35.
 
[38]  Adeniyi P.O. and Sanusi R.A. (2014). Efficacy of ginger (Zingiber officinale) extracts in lowering blood glucose in normal and High Fat diet-induced diabetic rats. American Journal of Food and Nutrition; 2(4): 55-58.