American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: Editor-in-chief: Hari K. Koul
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American Journal of Biomedical Research. 2015, 3(3), 53-57
DOI: 10.12691/ajbr-3-3-4
Open AccessArticle

Serum Estrogen and Estrogen Receptor Beta Levels in Female and Male Patients with Vitiligo

Nagwan A. Sabek1, , Moustafa M Eyada2, Shiymaa M Abdel Aziz2, Shimaa M Demerdash2, Amal H. Goma2 and Shereen Fikry2

1Medical Biochemistry Department, Suez Canal University, Ismailia, Egypt

2Dermatology & Venereology Department, Suez Canal University, Ismailia, Egypt

Pub. Date: September 06, 2015

Cite this paper:
Nagwan A. Sabek, Moustafa M Eyada, Shiymaa M Abdel Aziz, Shimaa M Demerdash, Amal H. Goma and Shereen Fikry. Serum Estrogen and Estrogen Receptor Beta Levels in Female and Male Patients with Vitiligo. American Journal of Biomedical Research. 2015; 3(3):53-57. doi: 10.12691/ajbr-3-3-4


Background: Vitiligo is a common skin disease characterized by cutaneous white macules. The exact pathogenesis of vitiligo is not yet known. Estrogen receptor β (ERβ) was expressed in the melanocytes and has a possible role in pathogenesis of autoimmune diseases. Objective: This study aimed to assess serum estrogen and ERβ levels in female and male vitiligo patients and studied their association with the disease activity and severity; to our knowledge this study is the first one that assessed the serum level of estrogen and estrogen receptor β in patients with vitiligo. Subjects and Method: the study was conducted on 30 female and 30 male patients with vitiligo and 30 female and 30 male healthy controls. All patients were subjected to full history taking and clinical assessment; serum estrogen and ERβ were measured using enzyme linked immune-assay (ELISA) kits. Results: Our results showed that serum estrogen level was statistically significant higher in female vitiligo patients compared to controls (46.3± 42.0 vs 30.0±28.9, P=0.028 respectively). Serum ERβ level was statistically lower in vitiligo patients compared to controls (96.5±52.2 vs 118.2 ± 76.0, P=0.035, respectively), also there was significantly difference in serum ERβ levels among different pattern of vitiligo (P=0.049), also we found serum estrogen levels were significantly higher in male vitiligo patients compared to controls (30.3 ±17.9 pg/ml, 21.3±11pg/ml, p=0.022). There was statistically significant difference in serum estrogen level in patients with different disease pattern (P=0.003). Conclusions: In our study population, there was a statistically significant difference in serum estrogen and ERβ in vitiligo female and male patients compared to their controls, these results might be highlight their possible role in the pathogenesis of vitiligo.

Vitiligo Estrogen Estrogen receptor β (ERβ) melanocytes vitiligo area severity index (VASI) vitiligo disease activity score (VIDA) enzyme linked immune-assay (ELISA)

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[1]  Kyriakis KP, Palamaras I, Tsele E, Case detection rates of vitiligo by gender and age. Int J Dermatol, 2009; 48: 328-329.
[2]  Tamer E, M.N. Ilhan, M. Polat, N. Lenk, N. Alli: Prevalence of skin diseases among pediatric patients in Turkey .J Dermatol, 2008, 35: 413-418.
[3]  Moscher, DB. Vitiligo etiology, pathogenesis, diagnosis and treatment. In Fitzpatrick TB, Eisen AZ, Wolff K, eds. Dermatology in General Medicine. 6th ed. New York: Mc Graw Hill. 2003; 839-47.
[4]  Taieb A and Picardo M: Clinical practice. Vitiligo. N Engl J Med, (2009); 360:160-169.
[5]  Alkhateeb, A., Fain, PR., Thody, T., Bennett, D.C., & Spritz, R.A: Vitiligo and associated autoimmune diseases in Caucasian probands and their families. Pigment Cell Res. (2003) 16:208-14.
[6]  Cunningham, M., & Gilkeson, G. Estrogen receptors in immunity and autoimmunity. Clinical reviews in allergy & immunology, (2011), 401, 66-73.
[7]  Taylor Ah, Al- Azzawi F. Immunolocalisation of oestrogen receptor beta in human tissues. J Mol Endocrinol 2000: 24: 145-155.
[8]  Taylor A H. Action of sex steroid hormones. In: Al-Azzawi F, Wahab M, eds. Hormone replacement therapy and the endometrium. New York: Parthenon Publishing, 2001: 49-70.
[9]  Enmark E, Pelto-Huikko M, Grandien K et al. Human estrogen receptor beta-gene structure, chromosomal localization, and expression pattern. J Clin Endocrinol Metab 1997: 82: 4258-4265.
[10]  Hasselquist M B, Goldberg N, Schroeter A, Spelsberg T C. Isolation and characterisation of the estrogen receptor in human skin. J Clin Endocrinol Metab 1980: 50: 76-82.
[11]  Phiel KL, Henderson RA, Adelman SI, Elloso MM, different estrogen receptor gene expression in human peripheral blood mononuclear cell population-immunol lett (2005): 97:107-113.
[12]  Jimbow k, Quevedo WC, Fitzpatrick TB,Szabo G 1999 in dermatology in general medicine 4 edition (MCGraw Hill Inc, New York, 261.
[13]  Hearing VJ: The regulation of melanin production, in the pigmentary system: physiology and pathology 1999 (Oxford University Press. New York).
[14]  Salzer BA, Schallreuter KU Investigation of the personality structure in patients with vitiligo and a possible association with impaired catecholamine metabolism. Dermatology (1995); 190: 109-15.
[15]  Pernis AB. Estrogen and CD4+ T cells. Curr Opin Rheumatol. 2007; 19: 414-20.
[16]  Anderson D, Schmid TE, Baumgartner A, Cemeli-Carratala E, Brinkworth MH,Wood JM Oestrogenic compounds and oxidative stress (in human sperm and lymphocytes in the Comet assay). Mutat Res.(2003) 544:173-8.
[17]  Ullrich, R. and Hofrichter, M. Enzymatic hydroxylation of aromatic compounds. Cellular and Molecular Life Sciences, 2007 64: 271-293.
[18]  Hamzavi, H. Jain, D. McLean, J. Shapiro, H. Zeng, and H. Lui, “Parametric modeling of narrowband UV-B phototherapy for vitiligo, using a novel quantitative tool: the Vitiligo Area Scoring Index,” Archives of Dermatology, vol. 140, no. 6:677-683, 2004.
[19]  Njoo, M. D. Das, P. K. Bos, J. D. and Westerhof, W. “Association of the Kobner phenomenon with disease activity and therapeutic responsiveness in vitiligo vulgaris,” Archives of Dermatology, 1999. 135, 4: 407-413.
[20]  Human Estrogen Receptor ER ELISA kit. Catalog No: E1050h. Retrieved from 2011.
[21]  Oxis International (2007) Inc 323 Vintage Park Dr. Foster City, CA 94404. ESTRADIOL (E2) ENZYME IMMUNOASSAY TEST KIT. (2007) Catalog Number: 11110.
[22]  Stephen, O. Vitiligo. the American Academy of Dermatology 1998:38. Issue 5, 647-668.
[23]  Whitton ME, Ashcroft DM, Barrett CW, Gonzalez U. Interventions for vitiligo. American Academy of Dermatology, 2008. 59, Issue 4, 713-717.
[24]  Jee, S. H., Lee, S. Y., Chiu, H. C., Chang, C. C., and Chen, T. J.: Effects of estrogen and estrogen receptor in normal human melanocytes. Biochem. Biophys. Res. Commun. (1994). 199, 1407-1412.
[25]  McLeod SD, Ranson M, Mason RS Effects of estrogens on human melanocytes in vitro. J Steroid Biochem Mol Biol 1994. 49: 9.
[26]  Yakimchuk K, Jondal M ,Okret S. Estrogen receptor α and β in the normal immune system and in lymphoid malignancies Mol Cell Endocrinol (2013); 15;375(1-2):121-9.
[27]  Doukas C, Saltiki K, Mantzou A, Cimponeriu A, Terzidis K, Sarika L, Mavrikakis M, Sfikakis P, Alevizaki M Hormonal parameters and sex hormone receptor gene polymorphisms in men with autoimmune diseases Rheumatol Int. (2012); 33(3):575-82.
[28]  Cutolo M , Sulli A, Straub RH. Estrogen metabolism and autoimmunity Autoimmun Rev; 11 (2012); (6-7):A460-4.
[29]  Cutolo M, Capellino S, Straub RH. Oestrogens in rheumatic diseases: friend or foe? Rheumatology (Oxford) (2008): 47 Suppl3:iii2.
[30]  Akihiro I, Hitoshi O, Toshio N , Iwao S ,Yoshinari T, Estrogen receptor expression by peripheral blood mononuclear cells of patients with systemic lupus erythematosus Clinical Rheumatology. 2007, Volume 26, Issue 10, pp 1675-1678.
[31]  Jin SY, Park HH, Li GZ, Lee HJ, Hong MS, Park HJ et al. Association of estrogen receptor 1 intron 1 C/T polymorphism in Korean vitiligo patients. J Dermatol Sci. 2004; 35:181.
[32]  Naresh C, Laddha , Mitesh Dwivedi , Mohmmad S. Mansuri , Amina R. Gani , Md Ansarullah ,A. V. Ramachandran , Sarat Dalai and Rasheedunnisa Begum Vitiligo: interplay between oxidative stress and immune system. Exp Dermatol. (2013); 22 (4):245-50.
[33]  Schallreuter KU, Chiuchiarelli G, Cemeli E, Elwary SM, et al., Estrogens can contribute to hydrogen peroxide generation and quinone-mediated DNA damage in peripheral blood lymphocytes from patients with vitiligo. J Invest Dermatol. 2006; 126 (5): 1036-42.
[34]  McMurray RW, May W Sex hormones and systemic lupus erythematosus: review and meta-analysis. Arthritis Rheum 2003; 48(8): 2100-2110.