American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: http://www.sciepub.com/journal/ajbr Editor-in-chief: Hari K. Koul
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American Journal of Biomedical Research. 2015, 3(3), 45-52
DOI: 10.12691/ajbr-3-3-3
Open AccessArticle

Effect of Variations in Post-set Temperature and Monomer Concentration on Self-cure Acrylic Surface Candidal Growth: An In-vitro Study

Nimra Tahir1, Faisal Moeen1, and Muhammad Humza Bin Saeed2

1Department of Dental Materials, Islamic International Dental College, Riphah University, Islamabad

2Department of Community Dentistry, Riphah International University

Pub. Date: July 29, 2015

Cite this paper:
Nimra Tahir, Faisal Moeen and Muhammad Humza Bin Saeed. Effect of Variations in Post-set Temperature and Monomer Concentration on Self-cure Acrylic Surface Candidal Growth: An In-vitro Study. American Journal of Biomedical Research. 2015; 3(3):45-52. doi: 10.12691/ajbr-3-3-3

Abstract

Objectives: To identify the effect of variations in power-liquid ratio and post-set temperature on water absorption and residual monomer concentration on growth of Candida albicans on self-cure denture base resins. Methodology: 60 self-cured acrylic discs (39 x 4mm) were made and divided into 3 groups each having 20 specimens. Group 1 consisted of discs fabricated at a powder-liquid ratio of 5.1:2.8 as recommended by the manufacturer. Groups 2 and 3 constituted specimens with variations in post-set temperatures and powder-liquid ratios respectively. Specimens from group 2 were soaked in four water baths at temperatures of 37°C, 47°C, 57°C and 67°C for 24 hours. Specimens from the temperature-controlled group 3 were fabricated at four different powder-liquid ratios by increasing monomer liquid volume by 10%, 15%, 20% and 25%. The acrylic discs from control groups 1 and 3 were soaked at a constant temperature of 37°C for 24 hours. Residual monomer leeched out from disc into water was analyzed using Ultraviolet spectrophotometer. The variations in water absorption and Candidal growth were recorded. Results: Linear regression analysis was used to analyze the results. A moderately positive correlation was calculated for the association between powder-liquid ratio and Candidal growth (r = 0.67, p < 0.001) suggesting Candidal growth is higher at increased powder-liquid ratios having low monomer content. The correlation between the Candidal growth and the post-set temperatures was found to be moderately negative (r = -0.41 p < 0.001), indicating a decrease in Candidal cells on increasing post-set soaking temperatures. Conclusions: Candidal growth follows a positive linear relation with a decreasing powder-liquid ratio having cells increasing with an increase in liquid monomer content. Candidal growth follows a negative linear relation with post-set temperature soaking with cells decreasing with an increase in temperature.

Keywords:
polymethyl-methacrylate (PMMA) self-cure acrylic resin residual monomer candida albicans water absorption

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