American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: http://www.sciepub.com/journal/ajbr Editor-in-chief: Hari K. Koul
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American Journal of Biomedical Research. 2015, 3(2), 29-34
DOI: 10.12691/ajbr-3-2-3
Open AccessArticle

Hepatoprotective, DNA Damage Prevention and Antioxidant Potential of Spirulina platensis on CCl4-Induced Hepatotoxicity in Mice

AshganA. AbouGabal1, Haiam M. Aboul-Ela2, , EmanM Ali3, Ahemd E.M khaled1 and Ola Kh. Shalaby2

1Genetics Branch, Botany Department, Faculty of Agriculture, Saba Basha, Alexandria University, Egypt

2Marine Biotechnology and Natural products lab, The National Institute of Oceanography and fisheries, Egypt

3Biophysics and Laser Science Unit, Basic Science Department, Research Institute of Ophthalmology

Pub. Date: June 15, 2015

Cite this paper:
AshganA. AbouGabal, Haiam M. Aboul-Ela, EmanM Ali, Ahemd E.M khaled and Ola Kh. Shalaby. Hepatoprotective, DNA Damage Prevention and Antioxidant Potential of Spirulina platensis on CCl4-Induced Hepatotoxicity in Mice. American Journal of Biomedical Research. 2015; 3(2):29-34. doi: 10.12691/ajbr-3-2-3

Abstract

In the present study, we have evaluated the hepatoprotective and antioxidant effects of Spirulina platensis against CCl4-induced hepatotoxicity in mice. Activities of liver marker enzymes; Alanine transaminase and Aspartate transaminasewere estimated, as well as lipid peroxidation and antioxidant status (glutathione peroxidase) were determined in liver homogenate. DNA damage in liver was also evaluated by means of Comet assay. CCl4 induction (1 mg/kg b.wt) significantly increases the levels of liver marker enzymesand lipid peroxidation, and caused the depletion of antioxidant status. Treatment of Spirulinaplatensis (800mg/kg/b.wt) to CCl4 challenged mice resulted in decreased liver marker enzymes activity, DNA damage and lipid peroxidation levels with increase in antioxidant status. Our study clearly demonstrates that Spirulina platensis shows hepatoprotective effect through its antioxidant activity on CCl4-induced hepatotoxicity in mice.

Keywords:
blue green algae lipid peroxidation liver marker enzymes mice comet assay

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