American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: Editor-in-chief: Hari K. Koul
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American Journal of Biomedical Research. 2015, 3(2), 29-34
DOI: 10.12691/ajbr-3-2-3
Open AccessArticle

Hepatoprotective, DNA Damage Prevention and Antioxidant Potential of Spirulina platensis on CCl4-Induced Hepatotoxicity in Mice

AshganA. AbouGabal1, Haiam M. Aboul-Ela2, , EmanM Ali3, Ahemd E.M khaled1 and Ola Kh. Shalaby2

1Genetics Branch, Botany Department, Faculty of Agriculture, Saba Basha, Alexandria University, Egypt

2Marine Biotechnology and Natural products lab, The National Institute of Oceanography and fisheries, Egypt

3Biophysics and Laser Science Unit, Basic Science Department, Research Institute of Ophthalmology

Pub. Date: June 15, 2015

Cite this paper:
AshganA. AbouGabal, Haiam M. Aboul-Ela, EmanM Ali, Ahemd E.M khaled and Ola Kh. Shalaby. Hepatoprotective, DNA Damage Prevention and Antioxidant Potential of Spirulina platensis on CCl4-Induced Hepatotoxicity in Mice. American Journal of Biomedical Research. 2015; 3(2):29-34. doi: 10.12691/ajbr-3-2-3


In the present study, we have evaluated the hepatoprotective and antioxidant effects of Spirulina platensis against CCl4-induced hepatotoxicity in mice. Activities of liver marker enzymes; Alanine transaminase and Aspartate transaminasewere estimated, as well as lipid peroxidation and antioxidant status (glutathione peroxidase) were determined in liver homogenate. DNA damage in liver was also evaluated by means of Comet assay. CCl4 induction (1 mg/kg b.wt) significantly increases the levels of liver marker enzymesand lipid peroxidation, and caused the depletion of antioxidant status. Treatment of Spirulinaplatensis (800mg/kg/b.wt) to CCl4 challenged mice resulted in decreased liver marker enzymes activity, DNA damage and lipid peroxidation levels with increase in antioxidant status. Our study clearly demonstrates that Spirulina platensis shows hepatoprotective effect through its antioxidant activity on CCl4-induced hepatotoxicity in mice.

blue green algae lipid peroxidation liver marker enzymes mice comet assay

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[1]  Abd El-Baky HH, El Baz FK, El-Baroty GS (2007) Enhancement of Antioxidant Production in Spirulina platensis under Oxidative Stress. ActaPhysiologiaePlantarum 31: 623-631.
[2]  Abdel-Daim MM, Abuzead SMM, Halawa SM (2013) Protective role of Spirulina platensis against acute deltamethrin-induced toxicity in rats. Plos one 8: e72991..
[3]  Abraham P (2005) Oxidative stress in paracetamol induced pathogenesis: (I). Renal damage. Indian Journal of Biochemistry and Biophysics 42:59-62.
[4]  Alam MA, Haider N, Ahmed S, Alam MT, Aziz A, Perveen A (2013) Tahlab (Spirulina) and few other medicinal plants having anti-oxidant &immunomodulatory properties described in Unani medicine- A review. International Journal of Pharmaceutical Sciences and Research 4: 4158-4164.
[5]  Ames BN, Shigenage MK, Hagen TM. Oxidants, antioxidants and the degenerative diseases of aging. Proceed Nat Acad Sci USA. 1993; 90(17):7915-7922.
[6]  Ayehunie S, Belay A, Baba TW, Ruprecht RM (1998) Inhibation of HIV-1 replication by an aqueous extract of Spirulina platensis (Arthrospira platensis). Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology 18: 7-12.
[7]  Bhat VB, Madyastha M (2001) Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: protection against oxidative damage to DNA. Biochemical and Biophysical Research Communications 285: 262-266.
[8]  Bhat, V.B. and Madyastha, K.M. 2000. C-phycocyanin: a potent peroxyl radical scavenger in vivo and in vitro. Biochem. Biophys. Res. Commun, (275): 20-25.
[9]  Chaung, S. S., Lin C. C., Lin, J., Yu, K. H., Hsu, Y. F., and Yen, M. H. (2003) “The hepatoprotective effects of Limoniumsinense against carbon tetrachloride and beta-D-galactosamine intoxication in rats,”Phytotherapy Research, vol. 17, pp. 784-791.
[10]  Deepti G, Shabad P, Dua KK (2011) Prevention of cadmium bioaccumulation by herbal adaptogen: Spirulina platensis. Journal of Chemical and Pharmaceutical Research 3: 603-608.
[11]  Duncan, D. B. (1955). Multiple range and multiple F test. Biometrics.,11: 1-42.
[12]  Ferrira-Hermosillo A,Torres-Duràn PV, Shamosh-Halabe S, Juàrez-Oropeza MA (2011) Biological effects of Spirulina and current research on its antioxidant activity. RevistaInternacional de CienciayTecnologiaBiomédica. Toctli RICTB, 2:1.
[13]  Fridovich I, (1983) Superoxide radical: An endogenous toxicant. Annual Review of Pharmacology and Toxicology 23:239-257.
[14]  Galal RM, Zaki HF, Seif El-Nasr MM, Agha AM (2012) Potential protective effect of honey against paracetamol-induced hepatotoxicity. Archives of Iranian Medicine 15:674-680.
[15]  Gini C. Kuriakose and Muraleedhara G. Kurup, 2010. Hepatoprotective effect of Spirulina lonar on paracetamol induced liver damage in rats. Asian journal biology science. 1(3), 614-623.
[16]  Halliwell B. Why and how should we measure oxidative DNA damage in nutritional studies? How far have we come? Am J ClinNutr. 2000;72(5):1082-1087.
[17]  Hemalatha K, Pugazhendy K, Jayachandran K, Jayanthi C, Meenambal M (2012) Studies on the Protective Efficacy of Spirulina against Lead Acetate Induced Hepatotoxicity in Rattusnorvegicus. International Journal of Chemical and Analytical Science 3: 1509-1512.
[18]  Hurkadale PJ, Shelar PA Palled SG, Mandavkar YD Khedkar, AS (2012) Hepatoprotective activity of Amorphophalluspaeoniifolius tubers against paracetamol-induced liver damage in rats. Asian Pacific Journal of Tropical Biomedicine 2: S238-S242.
[19]  Ibrahim M, Khaja ZU, Narasu ML (2011) Hepatoprotective activity of Boswelliaserrata extracts: in vitro and in vivo studies. International Journal of Pharmaceutical Applications 2:89-98.
[20]  Jackson, A.L. and Loeb, L.A. (2001) The contribution of endogenous sources of DNA damage to the multiple mutations in cancer. Mutation Research 477, 7-21.
[21]  Jarouliya U, Zacharia JA, Kumar P, Bisen PS, Prasad GB (2012) Alleviation of metabolic abnormalities induced by excessive fructose administration in wistar rats by Spirulina maxima. Indian Journal of Medical Research 135: 422-428.
[22]  Kaji, T., Fujiwara, Y. and Inomata, Y. 2002. Repair of wounded monolayers of cultured bovine aortic endothelial cells is inhibited by calcium spirulina, a novel sulfated polysaccharide isolated from Spirulina platensis. Life Sci., 70:1841-1848.
[23]  Khan M, Shobha JC, Mohan IK, Naidu MU, Sundaram C, Singh S, Kuppusamy P, Kutala VK (2005) Protective effect of Spirulina against doxorubicin-induced cardiotoxicity. Phytotherapy Research 19: 1030-1037.
[24]  Lalitsingh Ranawat, Jigar Bhatt, Jagruti Patel. (2010). Hepatoprotective activity of ethanolic extracts of bark of Zanthoxylumarmatum DC in CCl induced hepatic damage in rats. Journal of Ethnopharmacology 127: 777-780.
[25]  Mayuren C, Reddy VV, Priya SV, Devi VA (2010) Protective effect of Livactine against CCL4 and paracetamol induced hepatotoxicity in adult Wister rats. North American Journal of Medical Sciences 2:492-495.
[26]  Murugesh KS, Yeligar VC, Maiti BC and Maity TK (2005). Hepatoprotective and Antioxidant Role of BerberistinctoriaLesch leaves on Paracetamol induced hepatic damage in rats. Iranian Journal of Pharmacology and Therapeutics 4(1) 64-69.
[27]  Nan, P., Xiao X., Yan D.U., Jian C. and Zhong, C. 2013. Genotoxic effects of 8-hydroxylquinoline in loach (Misgurnusanguillicaudatus) assessed by the micronucleus test, comet assay and RAPD analysis. Environmental toxicology and pharmacology, (35): 434-443.
[28]  Nandhakumar S, Parasuraman S, Shanmugam M M, RamachandraRao K, Chand P, Bhat B V. (2011). Evaluation of DNA damage using single-cell gel electrophoresis (Comet Assay). J PharmacolPharmacother; 2:107-11
[29]  Pari L, Suresh A (2008) Effect of grape (Vitisvinifera L.) leaf extract on alcohol induced oxidative stress in rats. Food and Chemical Toxicology 46: 1627-1634.
[30]  Parikh P, Mani U, Iyer U (2001) Role of Spirulina in the control of glycemia and lipidemia in type 2 diabetes Mellitus. Journal of Medicinal Food 4: 193-199.
[31]  Ponce-Canchihuamán JC, Pérez-Méndez O, Hernández-Muñoz R, Torres-Durán PV, Juárez-Oropeza MA (2010) Protective effects of Spirulina maxima on hyperlipidemia and oxidative-stress induced by lead acetate in the liver and kidney. Lipids in Health and Disease 9: 35.
[32]  Premkumar K, Abraham SK, Santhiya ST, Ramesh A (2004) Protective effect of Spirulina fusiformis on chemical induced genotoxicity in mice. Fitoterapia 75: 24-31.
[33]  Price, C.P. and Alberti, K.G.M.M. (1985). Biochemical Assessment of Liver Functions. In:Wright R. Millword-Sadler GH, Alberti KGMM, Karran S. Eds. Liver and Biliary Diseases. ed. London. Pp.455-494.
[34]  Recknagel, R.O., E.A. Jr. Glende, J.A. Dolak and R.L. Waller. (1989). Mechanism of carbon tetrachloride toxicity. Pharmacol. Ther., 43 :139-154.
[35]  Rehab M, Ibrahim M (2012) Evaluation of the effect of Spirulina against gamma irradiation induced oxidative stress and tissue injury in rats. International Journal of Applied Science and Engineering Research 1: 152-164.
[36]  Sabina EP, Samuel J, RajappaRamya S, Patel S, Mandal N, Pranatharthiiharan P, Mishra PP, Rasool M (2009) Hepatoprotective and antioxidant potential of Spirulina fusiformis on acetaminophen-induced hepatotoxicity in mice. International Journal of Integrative Biology 6: 1-5.
[37]  Sallie, R., Tredger, J.M., Willam, R., 1991. Drugs and the liver. Biopharm. Drug Dispos. 12, 251-259.
[38]  SAS (2001). SAS User’s Guide Statistic. SAS Version 8.2. Inc. Cary. NC. USA.
[39]  Saxena PS, Kumar M (2004) Modulatory potential of Spirulina fusiformis on testicular phosphatases in Swiss albino mice against mercury intoxication. Indian Journal of Experimental Biology 42: 998-1002.
[40]  Sharma, M.K., Sharma, A., Kumar, A. and Kumar, M. 2007. Evaluation of protective efficacy of Spirulina against mercury induced nephrotoxicity in Swiss albino mice. Food and Chemical Toxicology, (45): 879-887.