American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: Editor-in-chief: Hari K. Koul
Open Access
Journal Browser
American Journal of Biomedical Research. 2015, 3(1), 9-12
DOI: 10.12691/ajbr-3-1-3
Open AccessArticle

Identification and Characterization of Antigenic 36 Kda Outer Membrane Protein (OMP) of Salmonella entericaserovar Typhi (S.typhi) from Makassar, South Sulawesi, Indonesia

Cut Muthiadin1, Rosdiana Natsir2, Rosana Agus3, Muhammad Nasrum4, Ressy Dwiyanti5, Muhammad Sabir5, Yadi Yasir6 and Mochammad Hatta4,

1Department of Biology, Islam Government University, Makassar, Indonesia

2Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

3Department of Microbiology, Faculty of Life Sciences, Hasanuddin University, Makassar, Indonesia

4Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

5Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia

6Department of Microbiology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia

Pub. Date: March 17, 2015

Cite this paper:
Cut Muthiadin, Rosdiana Natsir, Rosana Agus, Muhammad Nasrum, Ressy Dwiyanti, Muhammad Sabir, Yadi Yasir and Mochammad Hatta. Identification and Characterization of Antigenic 36 Kda Outer Membrane Protein (OMP) of Salmonella entericaserovar Typhi (S.typhi) from Makassar, South Sulawesi, Indonesia. American Journal of Biomedical Research. 2015; 3(1):9-12. doi: 10.12691/ajbr-3-1-3


Typhoid fever caused by Salmonella entericaserovar Typhi (S. typhi), still remains a public health concern. A simple, rapid and early diagnostic test has been a long felt need for clinicians. Adh036 protein, discovered from Malang, Indonesia, has a candidate vaccine potential for Typhoid Fever. This study aims towards identification and characterization of 36 kda Outer Membrane Protein (OMP) from Makassar, South Sulawesi, Indonesia as a generalization protein test. Isolated bacteria were cultured in MacConkey medium and Brain Heart Infusion Broth (BHIB), and then harvested by using sarcosyl and sonication methods. Then, crude protein was purified by dialysis method using fractional ammonium sulfate precipitation and the protein contents were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophores (SDS-PAGE) for identification it’s molecular weight. The results, from five purified proteins, only three fractions show band at 36 kda, were 0-20%, 20-40%, and 40-60%.

Outer Membrane Protein (OMP) Salmonella entericaserovar Typhi (S. typhi) Purification SDS-PAGE

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Crump, J. A., S. P. Luby, and E.D. Mintz., 2004. The Global burden of Typhoid fever. Bull. W. H. O. 82: 346-353.
[2]  Pui, C. F., Wong, W. C., Chai, L. C., Tunung, R., Jeyaletchumi, P., Noor Hidayah, M. S., Ubong, A., Farinazleen, M. G., Cheah, Y. K. and Son, R. (2011). Review Article; Salmonella: a food borne pathogen. International Food Research Journal. 18: 465.
[3]  Sagi, S. S. K., Paliwal, P., A., Chittaranjan, M., Khan, N., Mustoorn, S. R., Ilavazhagan, G., Sawhney, R.C. and Banerjee, P. K. (2006). Studies on immunogenicity and protective efficacy of DnaJ of Salmonella Typhi against lethal infection by Salmonella Typhimurium in mice. Vaccine, 24: 7135-7141.
[4]  Hamid, N. and Jain, S. K (2008). Characterization of an outer membrane protein of Salmonella entericaserovar Typhimurium that confers protection against Typhoid. Clinical and Vaccine Immunology, 15: 1461-1471.
[5]  Aslam MS, Akhter M, Rasheed R, Samra ZQ, Gull I, Athar MA, 2012. Identification and purification of antigenic 34 kDa outer membrane protein of Salmonella typhi. Pubmed. Clin Lab.; 58 (9-10): 1071-7
[6]  Kaur, J, and Jain, S.K. 2013. High Level Expression of 49kDa Outer Membrane Protein of Salmonella entericaserovar Typhi. Annals of Biological Research, 4 (1): 107-117.
[7]  Charles, S.D., Hussain, I., Choci, C.U., Nagaraja, K.V. and Sivanandan, V. (1994) A djuvanted subunit vaccines for the control of Salmonella Enteritidis infection in turkeys. Avian Dis 37, 477-484.
[8]  WHO. Initiative for Vaccine Research. Typhoid Fever. Accessed 2014- 12 January, html.
[9]  Winarsih, Sri. Production of Interleukin-12 in Induction By adhesin protein of Salmonella TyphiAdh O36. JurnalImuHayati. Vol. 12 no. 2 December 2005,: 155-161.
[10]  Winarsih, Sri. 2007. Cloning and expression gene Adhesin Adho36 of S.Typhi as candidate vaccines of Typhoid fever. Report of Research. RISTEK
[11]  MacConkey A.T. 1900. Note on a new medium for the growth and differentiation of the bacillus coli communis and the bacillus typhiabdominalis. Vol. 156. No. 4010. hal 1-72.
[12]  C.D.C., W. H. O. (2003). Manual for the Laboratory Identification and Antimicrobial Susceptibility Testing of Bacterial Pathogens of Public Health Importance in the Developing World. 103-110.
[13]  Kim, S., Kim, H., Reuhs, B.L and Mauer L,J. (2006). Differentiation of Outer Membrane Proteins from Salmonella entericaserotypes using Fourier transform infrared spectroscopy and chemometrics. Journal compilation Microbiology. 42: 229-234.
[14]  Malik, M., Butchaiah, G., Bansal, M. P., Siddiqui, M.Z. and Bakshi, C.S (1999) Sonicated extracts and outer membrane protein of Salmonella enteritidis strains and other Salmonella serovars. Indian J AnimSci 69, 788-789.
[15]  Davidson, L., Brear, D., Wingard, P., Hawkins, J., and Kito, G., 1977, Purification and Properties of an L-Glutaminase L-Asparaginase from Pseudomonas acidovorans. Journal of Bacteriology, Vol. 129.
[16]  Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall.1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
[17]  Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
[18]  Smith, et al. 2011. Application of a point-of-care test for the serodiagnosis of typhoid fever in Nigeria and the need for improved diagnostics. Journal Infect. Rev. 5 (7). 520-526.
[19]  Puspa W, Phihatini&Prohoesodo. 2005. Effectiveness of Widal Tube Test Using Import Antigen and local antigen. Indonesian Journal of clinical pathology and medical laboratory. Vol 12. No 1. 31-37.
[20]  Hamid, T.2001. Biological characterization of the outer membrane proteins of S. typhiand S. typhimurium and studies on their role in protection against typhoid. Ph.D. thesis. JamiaHamdard University, New Delhi, India.
[21]  Hobbs, et al. 2009. Evaluation of procedures for outer membrane isolation from Campylobacter jejuni. Microbiology. (155), 979-988.
[22]  Dubray G and G Bezard, 1980. Isolation of three protective cell wall antigens of Brucellaabortus in experimental brucellosis in mice. Ann Vet Res, 11: 367-373.
[23]  Santos JM, DR Verstreate, VY Perera and AJ Winter, 1984. Outer membrane proteins from rough strains of four Brucella species. Infect Immun, 46: 188-194.
[24]  Friedman, M., 2003, Chemistry, Biochemistry and Safety of Acrylamide, A Review, Journal Agric. Food. Chem, Vol. 51.
[25]  Lehninger, J. L., 1990, Biochemistry, Erlangga, Jakarta.
[26]  Hancock, R. E. W. &Nikaido, H. (1978). Outer membranes of gramnegative bacteria. XIX. Isolation from Pseudomonas auruginosa PAO1 and use in reconstitution and definition of the permeability barrier.JBacteriol 136, 381-390.
[27]  Osborn MJ, Gander JE, Parisi E, Carson J.Mechanism of assembly of the outer membrane of Salmonellatyphimurium. Isolation and characterization of cytoplasmic and outer membrane. J BiolChem 1972; 247: 3962-72.
[28]  Agustina, Dini. 2010. Identification of Outer Membrane Protein 36 kDa of S. Typhi from Malang and Various region. Journal Medika. Medical Faculty, Brawijaya University.