American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: http://www.sciepub.com/journal/ajbr Editor-in-chief: Hari K. Koul
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American Journal of Biomedical Research. 2015, 3(1), 9-12
DOI: 10.12691/ajbr-3-1-3
Open AccessArticle

Identification and Characterization of Antigenic 36 Kda Outer Membrane Protein (OMP) of Salmonella entericaserovar Typhi (S.typhi) from Makassar, South Sulawesi, Indonesia

Cut Muthiadin1, Rosdiana Natsir2, Rosana Agus3, Muhammad Nasrum4, Ressy Dwiyanti5, Muhammad Sabir5, Yadi Yasir6 and Mochammad Hatta4,

1Department of Biology, Islam Government University, Makassar, Indonesia

2Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

3Department of Microbiology, Faculty of Life Sciences, Hasanuddin University, Makassar, Indonesia

4Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

5Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia

6Department of Microbiology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia

Pub. Date: March 17, 2015

Cite this paper:
Cut Muthiadin, Rosdiana Natsir, Rosana Agus, Muhammad Nasrum, Ressy Dwiyanti, Muhammad Sabir, Yadi Yasir and Mochammad Hatta. Identification and Characterization of Antigenic 36 Kda Outer Membrane Protein (OMP) of Salmonella entericaserovar Typhi (S.typhi) from Makassar, South Sulawesi, Indonesia. American Journal of Biomedical Research. 2015; 3(1):9-12. doi: 10.12691/ajbr-3-1-3

Abstract

Typhoid fever caused by Salmonella entericaserovar Typhi (S. typhi), still remains a public health concern. A simple, rapid and early diagnostic test has been a long felt need for clinicians. Adh036 protein, discovered from Malang, Indonesia, has a candidate vaccine potential for Typhoid Fever. This study aims towards identification and characterization of 36 kda Outer Membrane Protein (OMP) from Makassar, South Sulawesi, Indonesia as a generalization protein test. Isolated bacteria were cultured in MacConkey medium and Brain Heart Infusion Broth (BHIB), and then harvested by using sarcosyl and sonication methods. Then, crude protein was purified by dialysis method using fractional ammonium sulfate precipitation and the protein contents were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophores (SDS-PAGE) for identification it’s molecular weight. The results, from five purified proteins, only three fractions show band at 36 kda, were 0-20%, 20-40%, and 40-60%.

Keywords:
Outer Membrane Protein (OMP) Salmonella entericaserovar Typhi (S. typhi) Purification SDS-PAGE

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