American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: http://www.sciepub.com/journal/ajbr Editor-in-chief: Hari K. Koul
Open Access
Journal Browser
Go
American Journal of Biomedical Research. 2014, 2(3), 47-51
DOI: 10.12691/ajbr-2-3-2
Open AccessArticle

The Pattern of Parasite Density, Plasma Total Bile Acids and Lactate Dehydrogenase in Plasmodium Infected Patients in Rural Community

Mathew Folaranmi OLANIYAN1, and Elizabeth Moyinoluwa BABATUNDE1

1Department of Medical Laboratory Science, Achievers University, Owo, Ondo state –Nigeria

Pub. Date: July 09, 2014

Cite this paper:
Mathew Folaranmi OLANIYAN and Elizabeth Moyinoluwa BABATUNDE. The Pattern of Parasite Density, Plasma Total Bile Acids and Lactate Dehydrogenase in Plasmodium Infected Patients in Rural Community. American Journal of Biomedical Research. 2014; 2(3):47-51. doi: 10.12691/ajbr-2-3-2

Abstract

Background to the study: Pathophysiology of Plasmodium (vivax, ovale, falciparum and malariae) infection involves liver. Liver dysfunction and destruction of tissues could be indicated by the plasma level of Total Bile Acid (TBA) and Lactate Dehydrogenase (LDH). Aim and Objective: This work was designed to evaluate the pattern of parasite density, plasma total bile acids, and Lactate dehydrogenase in Plasmodium infected patients in rural community. Materials and Methods: The study was carried out in Kishi, the Headquarter of Irepo Local government area of Oyo state - Nigeria. Seven hundred and nine (709) subjects (Female: n=403: male: n=306) aged 5 to 68 years were tested for Plasmodium infection using Giemsa- thick film technique. The overall prevalence of Plasmodium infection among the seven hundred and nine subjects screened was found to be 29.1% (206) including 12.97% (92) HIV, HBsAg and anti-HCV seronagative patients and 16.1% (114) HIV, HBsAg or anti-HCV seropositive patients. Ninety two (92 (12.97%)) that were HIV, HBsAg and anti-HCV seronagative(female-58 (63.0%); male-34 (37%)) were recruited out of 206 (29.1%) that were found to be infected with plasmodium spp for the study. None of the subject was jaundiced as at the time of sample collection. Hepatitis B surface antigen (HBsAg) and anti-HCV tests were carried out by Enzyme Linked Immunozorbent Assay (ELIZA). HIV screening and confirmation were carried out by immuno-chromatographic and Immunobloting (Western blot) assays respectively. Fasting Plasma Total Bile Acids (TBA) and Lactate Dehydrogenase (LDH) were analyzed in the patients biochemically by spectrophotometry. Result: The result obtained showed an overall prevalence of Plasmodium infection as 29.1% (206) (female: 107 (52%); male: 99 (48%)) including 12.97% (92) (Female: 58 (63.0%) Male: 34 (37%)) that were HIV, HBsAg and anti-HCV seronagative and 16.1% (114) (Female: 61 (53.5%); Male: 53 (46.5%)) were co-infected with at least one of HIV, HBV or HCV. The plasmodium infected subjects were grouped into three based on the parasite density such as: patients with parasite density of 50-499; 500-999 and ≥1000. The mean value of the parasite density of each group was correlated with the plasma level of LDH and TBA. In all groups there was a strong positive correlation(R=1; R2 =1) between the plasma TBA, LDH and plasmodium parasite density. The pattern of parasite density obtained in the rural community studied include 45.7% had a mean parasite density of 282±12.0; 43.5% (853±31.0) and 10.9% (1130±61.0). There was also a statistical significant increase in the mean value of LDH and TBA with increase in parasite density with p<0.05. Conclusion: This work showed an overall prevalence of 29.1% (206) plasmodium infection including 16.1% (114) of the patients co-infected with at least one of HIV, HBV or HCV. The plasma level of LDH and TBA was also found to be positively correlated and directly proportional to the parasite density. Evaluation of these parameters is therefore recommended for effective control and management.

Keywords:
prevalence LDH TBA parasite density plasmodium correlation

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Brian M. Greenwood, David A. Fidock, Dennis E. Kyle, Stefan H.I. Kappe, Pedro L. Alonso, Frank H. Collins, Patrick E. Duffy. Malaria: progress, perils, and prospects for eradication. J. Clin. Invest.; 118: 1266-1276. 2008. Full Text at http://www.jci.org/articles/view/33996/files/pdf
 
[2]  Azuma M, Shi M, Danenberg KD, Gardner H, Barrett C, Jacques CJ et al. "Serum lactate dehydrogenase levels and glycolysis significantly correlate with tumor VEGFA and VEGFR expression in metastatic CRC patients.". Pharmacogenomics: 8 (12): 1705-13. 2007.
 
[3]  Russell DW. "The enzymes, regulation, and genetics of bile acid synthesis". Annu. Rev. Biochem.; 72: 13774, 2003.
 
[4]  Shima T1, Tada H, Morimoto M, Nakagawa Y, Obata H, Sasaki T, Park H, Nakajo S, Nakashima T, Okanoue T, Kashima K.Serum total bile acid level as a sensitive indicator of hepatic histological improvement in chronic hepatitis C patients responding to interferon treatment. J Gastroenterol Hepatol. Mar; 15 (3): 294-9, 2000.
 
[5]  Chiang JY. "Bile acids: regulation of synthesis". J. Lipid Res.: 50 (10): 1955-66. 2009. PMC 2739756. PMID 19346330.
 
[6]  Hofmann AF. "The continuing importance of bile acids in liver and intestinal disease". Arch. Intern. Med.: 159 (22): 2647-58, 1999.
 
[7]  Greenwood BM, Armstrong JRM. Comparison of two simple methods for determining malaria parasite density. Transactions of the Royal Society of Tropical Medicine and Hygiene.; 85: 186-188, 1991.
 
[8]  Cheesbrough, Monica. District Laboratory practice in tropical Countries part1. 2002: Cambridge low-price edition. Cambridge University Press.
 
[9]  UF Ekpo, AM Omotayo, MA Dipeolu. Sendentarization and the prevalence of malaria and anaemia among settled Fulani pastoralists in south-western Nigeria. Nigerian Journal of Parasitology Vol. 29 (2): pp. 125-130, 2008.
 
[10]  Jain. C, N.C. Mogra, Jhaman Mehta, Rishi Diwan, Gaurav Dalela.Comparison Of Seropositivity Of Hiv, Hbv, Hcv And Syphilis And Malaria In Replacement And Voluntary Blood Donors In Western India. IJCRR.; 5 (3): 43-46, 2013.
 
[11]  Allauddin Niazi, Mohammad Tahir, Khalid Farooq Danish, Muhammad Abid, Shazia. Low Seroprevalence of HBV, HCV, HIV and Malaria in Voluntary Non Remunerated Blood Donors. Ann. Pak. Inst. Med. Sci.; 4 (3): 162-164, 2008.
 
[12]  Oduola. T, Bello. I and Avwioro. G. Hepatotoxicity and nephrotoxicity evaluation in Wistar albino rats exposed to Morinda lucida leaf extract. N Am J Med Sci. 2 (5): 230-233. 2010
 
[13]  Uzuegbu U.E. and Emeka C.B. Changes in Liver Function Biomarkers among Malaria Infected Patients in Ikeja Lagos State, Nigeria. Current Research Journal of Biological Sciences: 3 (3): 172-174, 2010. 2011 ISSN: 2041-0778.
 
[14]  Bhalla A, Suri V, Singh V. "Malarial hepatopathy". Journal of Postgraduate Medicine: 52 (4): 315-20. 2006 PMID 17102560.