American Journal of Biomedical Research
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American Journal of Biomedical Research. 2014, 2(1), 7-10
DOI: 10.12691/ajbr-2-1-2
Open AccessArticle

Effect of Alcohol Consumption and Oxidative Stress and Its Role in DNA Damage

Neelesh Deshpande1, Sabitha Kandi2, Manohar Muddeshwar1 and K V Ramana3,

1Department of Biochemistry, Government Medical College, Nagpur, India

2Department of Biochemistry, Chalmeda Anandarao Institute of Medical Sciences,karimnagar, India

3Department of Microbiology, Prathima Institute of Medical Sciences, Karimnagar, India

Pub. Date: January 17, 2014

Cite this paper:
Neelesh Deshpande, Sabitha Kandi, Manohar Muddeshwar and K V Ramana. Effect of Alcohol Consumption and Oxidative Stress and Its Role in DNA Damage. American Journal of Biomedical Research. 2014; 2(1):7-10. doi: 10.12691/ajbr-2-1-2


Oxidative stress has been increasingly implicated in different stages of liver cirrhosis and has been found responsible for DNA damage. Alcohol consumption and oxidative stress have been linked with DNA damage and progression of disease, leading to the hypothesis that chronic alcoholism causes DNA damage. The study was aimed at evaluating the relation between alcohol consumption and relative oxidative damage in different stages of liver cirrhosis. The study included two groups based on severity of cirrhosis of liver; categorized as compensated and decompensated liver cirrhotic patients based on child Pugh criteria. All decompensated cirrhotic patients in the study group had significantly higher MDA levels (P < 0.001) associated with DNA Damage (P > 0.01) than those with compensated cirrhotic patients and control group who were not suffering from liver cirrhosis. These results highlighted a significant higher degree of DNA damage in decompensated cirrhotic patients associated with oxidative stress as shown from greater average DNA migration in decompensated cirrhotic patients than in the compensated cirrhotic patients with low level of oxidative stress. Thus these results suggest that increase in MDA levels may be associated with pathogenesis and progression of liver cirrhosis.

liver cirrhosis genotoxicity DNA damage oxidative stress

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[1]  Abou-Seif, M., Rabia, A, and Nasr, M. Anti oxidant status. Erythrocyte membrane lipid peroxidation and osmotic fragility in malignant lymphoma patients. Clin Chem Lab Med 2000; 38: 737-42.
[2]  Dias-Da-Motta, p., Arruda, V.R., Muscara, M.n., Saad, T.O., De Nucci, G and Costa, F. The release of nitric oxide and superoxide anion by neutrophils and mononuclear cells from patients with sickle cell anemia. Br J Hem 1996; 93: 333-40.
[3]  Ljubuncic, T., Tane, Z. and Bomzon, A. Evidence of a systemic phenomenon for oxidative stress in cholestatic liver disease. Gut 2000; 47: 710-6.
[4]  Nalini,G., Hariprasad C. and Narayanan VA.Oxidative stress in alcoholic liver disease. Indian J Med Res 1999; 110: 200-3.
[5]  Togashi, H., Shinzowa, H., Wakabayashi, H., Nakamura, T., Yamada, V., Takahashi, T. and Ishikawa, M. Activities of free oxygen radical scavenger enzymes in human liver. J Hepatol 1999; 11: 200-5.
[6]  Brooks, P.J. DNA Damage, DNA repair, and alcohol toxicity a review. Alcoholism, Clinical & Experimental Research. 1997; 21, 1073-1082.
[7]  Friedman, SL. The cellular basis of hepatic fibrosis. Mechanism and treatment strategies. N Engl J Med 1999; 328: 1828-35.
[8]  Svegliati-Baroni, G., Di Sario, A., Casini, A., Ferretti, G., D” Ambrosio, L., Ridolfi, F., Bolognini, L., Salzano, R., Orlandi, F. and Benedetti, A. The Na+/H+ Exchanger modulates the fibrogenic effect of oxidative stress in rat hapatitic stellate cells. J Hepatol 1999; 30: 868-75.
[9]  Sukamato, HC., Kim, W., Louz, Z., Horn, W and Sul, C. Role of Lipid peroxidation in vitro and in vivo models of liver fibrogenesis. Gastroenterology 1993; 104: 1012.
[10]  Neelesh Deshpande, Sabitha Kandi, P Venkata Bharath Kumar, K V Ramana, and Manohar Muddeshwar, “Effect of Alcohol Consumption on Oxidative Stress Markers and its Role in the Pathogenesis and Progression of Liver Cirrhosis.” American Journal of Medical and Biological Research 1, no. 4 (2013): 99-102. doi: 10. 12691/ajmbr-1-4-3.
[11]  H. Sies. Strategies of antioxidant defence. Eur. J. Biochem. 1993; 215, 213-219.
[12]  DIN Sherman R Williams, Liver damage: mechanisms and management, British Medical Bulletin (1994) Vol. 50, No.1; 124-138.
[13]  Poli, G. Liver damage due to free radicals. British Medical bulletin 1993; 49: 604-620.
[14]  Ghany M, Hoofnagle JH. Liver and Biliary tract disease. In: Fauci AS, Kasper DL editors. Harrison’s Principles of Internal Medicine. 17th ed. US: McGraw Hill; 2008; (2) P-1923.
[15]  Gerard-Monnier D, Erdehneier I, Chandiere J, Yadan JC. Method of Colorimetric Analysis of Malondialdehyde and 4 Hydroxy-2-Enaldehydes as indices of lipid peroxidation. 1998; patent Number: 5, 726, 063.
[16]  Singh, N.P., McCoy, M.T., Tice, (1988) A simple technique for quantitation of low levels of DNA damage in individual Cells. Experimental Cell Research 175, 184-191.
[17]  Tsukamoto, H., Lu S. C. Current concepts in the pathogenesis of alcoholic liver injury. FASEB J. 2001; 15, 1335-1349.
[18]  Wang, Y., Millonig, G., Nair, J., Patsenker, E., Stickel, F., Mueller S., Bartsch H., Seitz, HK. Ethanol-induced cytochrome P 4502 E 1 causes etheno-DNA lesions in alcoholic liver disease. Hepathology 2009; 50: 453-461.
[19]  Hodges, NJ, Green RM., chipman JK., Graham M. Induction of DNA Strand Breaks and oxidative stress in Hela cells by ethanol is dependent on CYP 2 E 1 expression. Mutagenesis 2007; 22 (3): 189-194.
[20]  Varga, M. How can free radicals cause damage to hepatic cells. A multidisciplinary approach. Drug and alcohol dependence 1991; 27: 117-119.
[21]  Raquel Retana-Ugalde, Mario Altamirano-Lozano and Victor Manuel Mendoza-Nunez. Is there a similarity between DNA damage in adults with chronic alcoholism and community-dwelling healthy older adults Alcohol and alcoholism (2007), 42 (2): 64-69.
[22]  Singh, N.P. and Khan, A.Acetaldehyde: genotoxicity and cytotoxicity in human lymphocytes. Mutation Research 1995; 337: 9-17.
[23]  Roma´n, J., Colell, A., Blasco, C., Caballeria, J., Pare´s, A., Rode´s, J., and Ferna´ndez-Checa, JC. Differiancial role of ethanol and acetaldehyde in the induction of oxidative stress in HEP G 2 cells: Effect on Transcription factors AP-1 and NF-kB. Hepatology 1999; 30: 1473-1480.