Applied Ecology and Environmental Sciences
ISSN (Print): 2328-3912 ISSN (Online): 2328-3920 Website: Editor-in-chief: Alejandro González Medina
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Applied Ecology and Environmental Sciences. 2015, 3(5), 136-139
DOI: 10.12691/aees-3-5-2
Open AccessArticle

Influence of Prolonged Food Deprivation on Ascorbic Acid Levels of the Brain and Liver in Clarias batrachus (LINN.)

Nayan Kumar Prasad1,

1Tribhuvan University, Nepal

Pub. Date: October 12, 2015

Cite this paper:
Nayan Kumar Prasad. Influence of Prolonged Food Deprivation on Ascorbic Acid Levels of the Brain and Liver in Clarias batrachus (LINN.). Applied Ecology and Environmental Sciences. 2015; 3(5):136-139. doi: 10.12691/aees-3-5-2


The present paper deals with the effect of starvation on ascorbic acid contents of brain and hepatic tissues in both the sexes of Clarias batrachus. The level of ascorbic acid content was found relatively higher in males than in females. The brain showed higher value of ascorbic acid in normal condition (43.24mg/100gm in male and 40.65mg/100gm in female) in comparison to the liver (17.59mg/100gm in male and 13.32mg/100gm in female). During prolonged food deprivation, the gradual decrease in ascorbic acid level has been noticed in both the types of tissues which can be related to enhanced gluconeogenesis. The higher depletion has been observed in liver (73% in male & 78% in female) and lower depletion in the brain (57% in male &48% in female) after 40 days of starvation.

ascorbic acid brain Clarias batrachus liver starvation

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[1]  Bal, P.A. and Kalyani, M., “Studies on the ascorbic acid content of fish tissues,” J. Anim. Morph. Physiol., 7. 162-166. 1960 a.
[2]  Bal, P.A. and Kalyani, M., “Studies on the ascorbic acid content of fish tissues,” J. Zool. Soc. India. 12. 216-219. 1960 b.
[3]  Borah, S. and Yadav, R.N.S., “Bio-chemical and haematological response to starvation in an air breathing fresh water teleost, Heteropneustes fossilis, (Bloch),” Indian J. Fish, 43. 307-310. 1996.
[4]  Briggs, M.H., “Some aspects of the metabolism of ascorbic acid in insects,” Comp. Biochem. Physiol. 5. 241-252. 1962.
[5]  Chin, P. and Shin, Y.K., “Effect of starvation on the total metabolism of Crangon affinis,” Bull. Korean Fish Soc. 25. 371-382. 1992.
[6]  Deng, H., Wang, J. and Zhang, X., “The changes in biochemical constituents in serum liver and muscular tissue of German mirror carp under different period of starvation,” J. Dalian Fish Coll. Dolian Shulchan Xneyuan Xnebao, 8. 57-61. 1993.
[7]  Dvorak, N., “Effect of corticotrophin, starvation and glucose on ascorbic acid levels in the blood plasma of piglets,” Nutr.Catabolism. 16. 215-222. 1974.
[8]  Fomin, S.V. and Romanjuk, N.M., “The ascorbic acid contents of the tissues of various fish,” Ukr. Biokhem. Zh., 10. 365-378. 1937.
[9]  Kanungo, M.S. and Patnaik, B.K., “Ascorbic acid and ageing in rat,” Biochem. J., 90. 637. 1964.
[10]  Kiran, S. and Talesara, C. L., “Prolonged starvation effects on myotomal muscle of two freshwater teleost fishes murrel (Channa punctatus) and catfish (Clarias batrachus),” Indian J. Exp. Biol., 23. 17-22. 1985.
[11]  Leblond, C.P., Giroud, A., Ratsimamange, R. and Gero, E., “The normal level of ascorbic acid,” Bull. Soc. Chim. Biol., 20. 1079-1087. 1938.
[12]  Letcher, B.H., Rice, J.A., Corowder, L.B. and Binkowski, F.P., “Size dependent effects of continuous and intermittent feeding on starvation time and mass loss in starving yellow perch larvae and juveniles,” Trans. An. Fish Soc. 125. 14-26. 1996.
[13]  Mueller, M.J., Paschan, U. and Seitz, H.J., “Role of thyroid hormones in the metabolic adaptation to starvation in the miniature pig,” Acta.Endocrinol. Suppl., 99(246). 76-77. 1982.
[14]  Mukhopadhyaya, P.K., Mohanty, S.N., Das, K.M., Sarkar, S. and Batra, B.C., “Growth and changes in carcass composition in young of Labeorohita and Cirrhinusmrigala during feeding and starvation,” in Fish Nutrition Research in Asia. Proc. of the 4th Asian Fish, Spec. Publ. Asian Fish. Soc., 5, 87-91. 1991.
[15]  Mustafa, S., “Changes in Biochemical composition in Starving Catfish, Heteropneustes fossilis,” Japanese Journal of Ichthyology, 29. 4. 1983.
[16]  Paratheswararao, V., “Some mechanisms underlying thermal acclimation in a fresh water fish, Etroalus maculates (Teleosti),” Comp. Biochem. Physio. 21. 619-625. 1967.
[17]  Prosser, C.L., Comparative Animal Physiology, 3rd Edition. Satish Book Enterprise Booksellers and Publishers, Agra. 1984.
[18]  Rajyasree, M., and Naidu, K.R.P., “Starvation induced changes in biochemical aspects of hepatic tissue of fish, Labeo rohita,” Indian J. Fish. 36. 339-341. 1989.
[19]  Roe, J.H., “Chemical determination of ascorbic and dehydroascorbic and diketogulonic acid,” In Methods of Biochemical Analysis, vol. i (ed. D. Glick.) New York, Interscience Publishers, 1954.
[20]  Shanta, N. and Motelica, I., “Influence of starvation on brain and liver cholesterol levels of the catfish Heteropneustes fossilis,” Proc. Indian Academy of Science, Sect. B. 881(1 part 3). 205-208. 1962.
[21]  Siddiqui, M.A., “Seasonal variation in the ascorbic acid content and calcium content in different tissues of Ophiocephalus punctatus (Bloch),” I. J. Exp. Biol., 5: 54-55. 1967.
[22]  Sinha, R.C., Effect of starvation on the concentration of ascorbic acid in the pedipalp muscle of scorpion, Palamnasus bengalensis,” Experiments, 22. 221. 1966.
[23]  Srivastava, G.J., Fishes of UP and Bihar, 11th ed. Vishwavidyalaya Prakashan, Varanasi, India. 2006.
[24]  Tripathi, G. and Verma, P., “Starvation-Induced Impairment of Metabolism in a Freshwater Catfish,” Z. Naturforsch. 58c. 446-451. 2003.
[25]  Wright, S., Applied physiology, Oxford Univ. Press, London. 12th edition, 204-466. 1976.